Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii
Abstract Background Behavioural shifts in the canonical location and timing of biting have been reported in natural populations of anopheline malaria vectors following the implementation of insecticide-based indoor vector control interventions. These modifications increase the likelihood of human-ve...
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doaj-53d1f4cfeddd4f0e844bd56128c6283f2021-09-12T11:27:35ZengBMCParasites & Vectors1756-33052021-09-0114111610.1186/s13071-021-04967-0Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzziiAmadou S. Traoré0Angélique Porciani1Nicolas Moiroux2Roch K. Dabiré3Frédéric Simard4Carlo Costantini5Karine Mouline6MIVEGEC, Univ Montpellier, CNRS, IRDMIVEGEC, Univ Montpellier, CNRS, IRDMIVEGEC, Univ Montpellier, CNRS, IRDInstitut de Recherche en Sciences de La Santé (IRSS)MIVEGEC, Univ Montpellier, CNRS, IRDMIVEGEC, Univ Montpellier, CNRS, IRDMIVEGEC, Univ Montpellier, CNRS, IRDAbstract Background Behavioural shifts in the canonical location and timing of biting have been reported in natural populations of anopheline malaria vectors following the implementation of insecticide-based indoor vector control interventions. These modifications increase the likelihood of human-vector contact and allow mosquitoes to avoid insecticides, both conditions being favourable to residual transmission of the malarial parasites. The biting behaviour of mosquitoes follows rhythms that are under the control of biological clocks and environmental conditions, modulated by physiological states. In this work we explore modifications of spontaneous locomotor activity expressed by mosquitoes in different physiological states to highlight phenotypic variability associated to circadian control that may contribute to explain residual transmission in the field. Methods The F10 generation progeny of field-collected Anopheles coluzzii from southwestern Burkina Faso was tested using an automated recording apparatus (Locomotor Activity Monitor, TriKinetics Inc.) under LD 12:12 or DD light regimens in laboratory-controlled conditions. Activity recordings of each test were carried out for a week with 6-day-old females belonging to four experimental treatments, representing factorial combinations of two physiological variables: insemination status (virgin vs inseminated) and gonotrophic status (glucose fed vs blood fed). Chronobiological features of rhythmicity in locomotor activity were explored using periodograms, diversity indices, and generalized linear mixed modelling. Results The average strength of activity, onset of activity, and acrophase were modulated by both nutritional and insemination status as well as by the light regimen. Inseminated females showed a significant excess of arrhythmic activity under DD. When rhythmicity was observed in DD, females displayed sustained activity also during the subjective day. Conclusions Insemination and gonotrophic status influence the underlying light and circadian control of chronobiological features of locomotor activity. Overrepresentation of arrhythmic chronotypes as well as the sustained activity of inseminated females during the subjective day under DD conditions suggests potential activity of natural populations of A. coluzzii during daytime under dim conditions, with implications for residual transmission of malarial parasites. Graphical abstracthttps://doi.org/10.1186/s13071-021-04967-0Anopheles coluzziiFieldLocomotor activityDaily rhythmsInseminationBlood and glucose intakes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amadou S. Traoré Angélique Porciani Nicolas Moiroux Roch K. Dabiré Frédéric Simard Carlo Costantini Karine Mouline |
spellingShingle |
Amadou S. Traoré Angélique Porciani Nicolas Moiroux Roch K. Dabiré Frédéric Simard Carlo Costantini Karine Mouline Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii Parasites & Vectors Anopheles coluzzii Field Locomotor activity Daily rhythms Insemination Blood and glucose intakes |
author_facet |
Amadou S. Traoré Angélique Porciani Nicolas Moiroux Roch K. Dabiré Frédéric Simard Carlo Costantini Karine Mouline |
author_sort |
Amadou S. Traoré |
title |
Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii |
title_short |
Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii |
title_full |
Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii |
title_fullStr |
Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii |
title_full_unstemmed |
Effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito Anopheles coluzzii |
title_sort |
effects of insemination and blood-feeding on locomotor activity of wild-derived females of the malaria mosquito anopheles coluzzii |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2021-09-01 |
description |
Abstract Background Behavioural shifts in the canonical location and timing of biting have been reported in natural populations of anopheline malaria vectors following the implementation of insecticide-based indoor vector control interventions. These modifications increase the likelihood of human-vector contact and allow mosquitoes to avoid insecticides, both conditions being favourable to residual transmission of the malarial parasites. The biting behaviour of mosquitoes follows rhythms that are under the control of biological clocks and environmental conditions, modulated by physiological states. In this work we explore modifications of spontaneous locomotor activity expressed by mosquitoes in different physiological states to highlight phenotypic variability associated to circadian control that may contribute to explain residual transmission in the field. Methods The F10 generation progeny of field-collected Anopheles coluzzii from southwestern Burkina Faso was tested using an automated recording apparatus (Locomotor Activity Monitor, TriKinetics Inc.) under LD 12:12 or DD light regimens in laboratory-controlled conditions. Activity recordings of each test were carried out for a week with 6-day-old females belonging to four experimental treatments, representing factorial combinations of two physiological variables: insemination status (virgin vs inseminated) and gonotrophic status (glucose fed vs blood fed). Chronobiological features of rhythmicity in locomotor activity were explored using periodograms, diversity indices, and generalized linear mixed modelling. Results The average strength of activity, onset of activity, and acrophase were modulated by both nutritional and insemination status as well as by the light regimen. Inseminated females showed a significant excess of arrhythmic activity under DD. When rhythmicity was observed in DD, females displayed sustained activity also during the subjective day. Conclusions Insemination and gonotrophic status influence the underlying light and circadian control of chronobiological features of locomotor activity. Overrepresentation of arrhythmic chronotypes as well as the sustained activity of inseminated females during the subjective day under DD conditions suggests potential activity of natural populations of A. coluzzii during daytime under dim conditions, with implications for residual transmission of malarial parasites. Graphical abstract |
topic |
Anopheles coluzzii Field Locomotor activity Daily rhythms Insemination Blood and glucose intakes |
url |
https://doi.org/10.1186/s13071-021-04967-0 |
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