Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study
Autism is a severe neurodevelopmental disorder. Many susceptible causative genes have been identified. Most of the previous reports showed the relationship between the Human Leukocyte Antigen (HLA) gene and etiology of autism. In order to identify HLA-B alleles associated with autism in Thai populat...
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doaj-53dbd51f7ed2484d94acf8eb78ce18cc2020-11-24T20:41:21ZengHindawi LimitedDisease Markers0278-02401875-86302015-01-01201510.1155/2015/724935724935Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control StudyApichaya Puangpetch0Pongwut Suwannarat1Montri Chamnanphol2Napatrupron Koomdee3Nattawat Ngamsamut4Penkhae Limsila5Chonlaphat Sukasem6Division of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDivision of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandYuwaprasart Waithayopathum Child and Adolescent Psychiatric Hospital, Department of Mental Health Services, Ministry of Public Health, Samut Prakarn 10270, ThailandYuwaprasart Waithayopathum Child and Adolescent Psychiatric Hospital, Department of Mental Health Services, Ministry of Public Health, Samut Prakarn 10270, ThailandDivision of Pharmacogenomics and Personalized Medicine, Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandAutism is a severe neurodevelopmental disorder. Many susceptible causative genes have been identified. Most of the previous reports showed the relationship between the Human Leukocyte Antigen (HLA) gene and etiology of autism. In order to identify HLA-B alleles associated with autism in Thai population, we compared the frequency of HLA-B allele in 364 autistic subjects with 952 normal subjects by using a two-stage sequence-specific oligonucleotide probe system (PCR-SSOP) method based on flow-cytometry technology. HLA-B⁎13:02 (P=0.019, OR = 2.229), HLA-B⁎38:02 (P=0.049, OR = 1.628), HLA-B⁎44:03 (P=0.016, OR = 1.645), and HLA-B⁎56:01 (P = 1.78 × 10−4, OR = 4.927) alleles were significantly increased in autistic subjects compared with normal subjects. Moreover, we found that the HLA-B⁎18:02 (P=0.016, OR = 0.375) and HLA-B⁎46:12 (P=0.008, OR = 0.147) alleles were negatively associated with autism when compared to normal controls. Both alleles might have a protective role in disease development. In addition, four HLA-B genotypes of autistic patients had statistically significant relationship with control groups, consisting of HLA-B⁎3905/⁎5801 (P=0.032, OR = 24.697), HLA-B⁎2704/⁎5801 (P=0.022, OR = 6.872), HLA-B⁎3501/⁎4403 (P=0.021, OR = 30.269), and HLA-B⁎1801/⁎4402 (P = 0.017, OR = 13.757). This is the first report on HLA-B associated with Thai autism and may serve as a marker for genetic susceptibility to autism in Thai population.http://dx.doi.org/10.1155/2015/724935 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Apichaya Puangpetch Pongwut Suwannarat Montri Chamnanphol Napatrupron Koomdee Nattawat Ngamsamut Penkhae Limsila Chonlaphat Sukasem |
spellingShingle |
Apichaya Puangpetch Pongwut Suwannarat Montri Chamnanphol Napatrupron Koomdee Nattawat Ngamsamut Penkhae Limsila Chonlaphat Sukasem Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study Disease Markers |
author_facet |
Apichaya Puangpetch Pongwut Suwannarat Montri Chamnanphol Napatrupron Koomdee Nattawat Ngamsamut Penkhae Limsila Chonlaphat Sukasem |
author_sort |
Apichaya Puangpetch |
title |
Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study |
title_short |
Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study |
title_full |
Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study |
title_fullStr |
Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study |
title_full_unstemmed |
Significant Association of HLA-B Alleles and Genotypes in Thai Children with Autism Spectrum Disorders: A Case-Control Study |
title_sort |
significant association of hla-b alleles and genotypes in thai children with autism spectrum disorders: a case-control study |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2015-01-01 |
description |
Autism is a severe neurodevelopmental disorder. Many susceptible causative genes have been identified. Most of the previous reports showed the relationship between the Human Leukocyte Antigen (HLA) gene and etiology of autism. In order to identify HLA-B alleles associated with autism in Thai population, we compared the frequency of HLA-B allele in 364 autistic subjects with 952 normal subjects by using a two-stage sequence-specific oligonucleotide probe system (PCR-SSOP) method based on flow-cytometry technology. HLA-B⁎13:02 (P=0.019, OR = 2.229), HLA-B⁎38:02 (P=0.049, OR = 1.628), HLA-B⁎44:03 (P=0.016, OR = 1.645), and HLA-B⁎56:01 (P = 1.78 × 10−4, OR = 4.927) alleles were significantly increased in autistic subjects compared with normal subjects. Moreover, we found that the HLA-B⁎18:02 (P=0.016, OR = 0.375) and HLA-B⁎46:12 (P=0.008, OR = 0.147) alleles were negatively associated with autism when compared to normal controls. Both alleles might have a protective role in disease development. In addition, four HLA-B genotypes of autistic patients had statistically significant relationship with control groups, consisting of HLA-B⁎3905/⁎5801 (P=0.032, OR = 24.697), HLA-B⁎2704/⁎5801 (P=0.022, OR = 6.872), HLA-B⁎3501/⁎4403 (P=0.021, OR = 30.269), and HLA-B⁎1801/⁎4402 (P = 0.017, OR = 13.757). This is the first report on HLA-B associated with Thai autism and may serve as a marker for genetic susceptibility to autism in Thai population. |
url |
http://dx.doi.org/10.1155/2015/724935 |
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