Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods
A spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artif...
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Iranian Chemical Society
2015-06-01
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doaj-53e2b6b6753a4cdf9940243d1817ebe42020-11-24T21:42:16ZengIranian Chemical SocietyAnalytical and Bioanalytical Chemistry Research2383-093X2383-093X2015-06-0121425110.22036/abcr.2015.92039203Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics MethodsTayyebeh Madrakian0Masoumeh Madadi-Shad1Mohammad Soleimani2Bu- Ali Sina University, Hamedan, 6517838683, IranDepartment of Chemistry, Saveh Branch, Islamic Azad University, Saveh , IranBu- Ali Sina University, Hamedan,6517838683, IranA spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. The method is based on the complexation reaction of MPM and MPA with Fe(III) ion in the solution. A nonionic surfactant, Triton X-100, was used for dissolving the complexes and intensifying the signals. The linear determination ranges for the determination of MPA and MPM were 5.0-215.0 mg l-1, and 10.0-1000.0 mg l-1, respectively. The detection limit for MPA and MPM was obtained as 0.3 mg l-1 and 1.1 mg l-1, respectively. Satisfactory results were obtained by the combination of spectrophotometric method and chemometrics techniques. The method was successfully applied to the simultaneous determination of MPM and MPA in serum sample and the results were comparable with HPLC method.http://www.analchemres.org/article_9203_3bcac61326a10247b928d1afc40e5722.pdfMycophenolate mofetilMycophenolic acidSimultaneous determinationChemometric methodsSpectrophotometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tayyebeh Madrakian Masoumeh Madadi-Shad Mohammad Soleimani |
spellingShingle |
Tayyebeh Madrakian Masoumeh Madadi-Shad Mohammad Soleimani Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods Analytical and Bioanalytical Chemistry Research Mycophenolate mofetil Mycophenolic acid Simultaneous determination Chemometric methods Spectrophotometry |
author_facet |
Tayyebeh Madrakian Masoumeh Madadi-Shad Mohammad Soleimani |
author_sort |
Tayyebeh Madrakian |
title |
Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods |
title_short |
Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods |
title_full |
Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods |
title_fullStr |
Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods |
title_full_unstemmed |
Simultaneous Spectrophotometric Determination of Mycophenolate Mofetil and Its Active Metabolite in Human Plasma Using Chemometrics Methods |
title_sort |
simultaneous spectrophotometric determination of mycophenolate mofetil and its active metabolite in human plasma using chemometrics methods |
publisher |
Iranian Chemical Society |
series |
Analytical and Bioanalytical Chemistry Research |
issn |
2383-093X 2383-093X |
publishDate |
2015-06-01 |
description |
A spectrophotometric method for selective complexation reaction and simultaneous determination of mycophenolate mofetil (MPM), and mycophenolic acid (MPA) using three multivariate chemometric methods, i.e. partial least squares regression, principal component regression and principal component artificial neural networks, is proposed. The method is based on the complexation reaction of MPM and MPA with Fe(III) ion in the solution. A nonionic surfactant, Triton X-100, was used for dissolving the complexes and intensifying the signals. The linear determination ranges for the determination of MPA and MPM were 5.0-215.0 mg l-1, and 10.0-1000.0 mg l-1, respectively. The detection limit for MPA and MPM was obtained as 0.3 mg l-1 and 1.1 mg l-1, respectively. Satisfactory results were obtained by the combination of spectrophotometric method and chemometrics techniques. The method was successfully applied to the simultaneous determination of MPM and MPA in serum sample and the results were comparable with HPLC method. |
topic |
Mycophenolate mofetil Mycophenolic acid Simultaneous determination Chemometric methods Spectrophotometry |
url |
http://www.analchemres.org/article_9203_3bcac61326a10247b928d1afc40e5722.pdf |
work_keys_str_mv |
AT tayyebehmadrakian simultaneousspectrophotometricdeterminationofmycophenolatemofetilanditsactivemetaboliteinhumanplasmausingchemometricsmethods AT masoumehmadadishad simultaneousspectrophotometricdeterminationofmycophenolatemofetilanditsactivemetaboliteinhumanplasmausingchemometricsmethods AT mohammadsoleimani simultaneousspectrophotometricdeterminationofmycophenolatemofetilanditsactivemetaboliteinhumanplasmausingchemometricsmethods |
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