Production of Resveratrol by Piceid Deglycosylation Using Cellulase

• Main Authors: Chia-Hung Kuo, Bao-Yuan Chen, Yung-Chuan Liu, Jiann-Hwa Chen, Chwen-Jen Shieh
• Format: Article
• Language: English
• Published: MDPI AG 2016-02-01
• Series: Catalysts
• Subjects: cellulase; piceid; resveratrol; Polygonum cuspidatum; enzymatic transformation
• Online Access: http://www.mdpi.com/2073-4344/6/3/32

Resveratrol is a dietary polyphenolic compound widely used in medicine, food, and cosmetic products. The glycoside form of resveratrol, piceid, is also present in several plant materials but is less bioavailable. In this study, enzymatic transformation of piceid into resveratrol using inexpensive cellulase was investigated. Response surface methodology was used to evaluate the effect of reaction parameters, including reaction temperature, reaction time, enzyme amount and pH. The optimal conditions for biotransformation of piceid to resveratrol are: a reaction temperature of 50 °C, reaction time of 4.75 h, enzyme amount of 2.5 fungal β-glucanase (FBG) units and pH of 4.3. In addition, the extracts from Polygonum cuspidatum root contained high amounts of piceid were treated with cellulase in order to deglycosylation that increased resveratrol yield. After treatment, the resveratrol yield significantly increased from 2.72 to 9.49 mg/g, while the piceid contents decreased from 8.60 to 0 mg/g. The result provides an efficient method to convert piceid in the extracts of P. cuspidatum root into resveratrol by cellulase.