Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach
Objective: Breast cancer (BC) still remains an imperative clinical issue, despite advances in the diagnosis, prognosis and treatment modalities of this malignancy. Hence, progress has been made to identify non-invasive, high sensitive and specific biomarkers. Since immune system affects development...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Royan Institute (ACECR), Tehran
2021-09-01
|
Series: | Cell Journal |
Subjects: | |
Online Access: | https://celljournal.org/journal/article/fulltext/identification-of-ccnb2-as-a-potential-non-invasive-breast-cancer-biomarker-in-peripheral-blood-mononuclear-cells-using-the-systems-biology-approach.pdf |
id |
doaj-53e710d46a554a5dbb1361bc846cb26f |
---|---|
record_format |
Article |
spelling |
doaj-53e710d46a554a5dbb1361bc846cb26f2021-09-12T07:34:15ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142021-09-0123440641310.22074/cellj.2021.7053Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology ApproachRaheleh Moradpoor0Hakimeh Zali1Ahmad Gharebaghian2Mohammad Esmaeil Akbari3Soheila Ajdari4Mona Salimi5Department of Basic Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranSchool of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences,Tehran, IranDepartment of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranCancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Immunology, Pasteur Institute of Iran, Tehran, IranDepartment of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, IranObjective: Breast cancer (BC) still remains an imperative clinical issue, despite advances in the diagnosis, prognosis and treatment modalities of this malignancy. Hence, progress has been made to identify non-invasive, high sensitive and specific biomarkers. Since immune system affects development of breast cancer, peripheral blood mononuclear cells (PBMCs) -a subpopulation of immune cells- can be considered as a promising tool in the field of BC biomarker research. In the current study, we initially attempted to use concept of the present shared biomarkers in solid tumors and systemic immune profile and then evaluate correlation of these biomarkers to clinical use in cancer research. Materials and Methods: In this experimental study, available microarray gene expression datasets of BC as well as the related PBMCs were retrieved and downloaded from the Gene Expression Omnibus (GEO) database, followed by analysis using GEO2R along with affylmGUI, a R-based package, to obtain differentially expressed genes (DEGs). Signature genes from 20 types of cancer were also applied to validate DEGs. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was carried out to assess mRNA level of CCNB2 in PBMC of the BC patients and healthy subjects. Results: DEGs analysis for the transcription profile of BC cells and PBMCs showed two shared targets, CCNB2 and PGK1. Validation with systems biology using reweighted 20 types of cancer signature genes revealed that CCNB2 is the only common target in BC and its related PBMCs, which was further validated by qRT-PCR implying a significant increase in the level of CCNB2 in the BC patients. Conclusion: Results of this study demonstrated that PBMCs are affected by BC cells and CCNB2 may be of value as a diagnostic biomarker for breast cancer. However, verification would require future detailed experimental plans.https://celljournal.org/journal/article/fulltext/identification-of-ccnb2-as-a-potential-non-invasive-breast-cancer-biomarker-in-peripheral-blood-mononuclear-cells-using-the-systems-biology-approach.pdfbiomarkerbreast cancerperipheral blood mononuclear cellsystems biology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raheleh Moradpoor Hakimeh Zali Ahmad Gharebaghian Mohammad Esmaeil Akbari Soheila Ajdari Mona Salimi |
spellingShingle |
Raheleh Moradpoor Hakimeh Zali Ahmad Gharebaghian Mohammad Esmaeil Akbari Soheila Ajdari Mona Salimi Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach Cell Journal biomarker breast cancer peripheral blood mononuclear cell systems biology |
author_facet |
Raheleh Moradpoor Hakimeh Zali Ahmad Gharebaghian Mohammad Esmaeil Akbari Soheila Ajdari Mona Salimi |
author_sort |
Raheleh Moradpoor |
title |
Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach |
title_short |
Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach |
title_full |
Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach |
title_fullStr |
Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach |
title_full_unstemmed |
Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach |
title_sort |
identification of ccnb2 as a potential non-invasive breast cancer biomarker in peripheral blood mononuclear cells using the systems biology approach |
publisher |
Royan Institute (ACECR), Tehran |
series |
Cell Journal |
issn |
2228-5806 2228-5814 |
publishDate |
2021-09-01 |
description |
Objective: Breast cancer (BC) still remains an imperative clinical issue, despite advances in the diagnosis, prognosis and treatment modalities of this malignancy. Hence, progress has been made to identify non-invasive, high sensitive and specific biomarkers. Since immune system affects development of breast cancer, peripheral blood mononuclear cells (PBMCs) -a subpopulation of immune cells- can be considered as a promising tool in the field of BC biomarker research. In the current study, we initially attempted to use concept of the present shared biomarkers in solid tumors and systemic immune profile and then evaluate correlation of these biomarkers to clinical use in cancer research. Materials and Methods: In this experimental study, available microarray gene expression datasets of BC as well as
the related PBMCs were retrieved and downloaded from the Gene Expression Omnibus (GEO) database, followed by analysis using GEO2R along with affylmGUI, a R-based package, to obtain differentially expressed genes (DEGs). Signature genes from 20 types of cancer were also applied to validate DEGs. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was carried out to assess mRNA level of CCNB2 in PBMC of the BC patients
and healthy subjects. Results: DEGs analysis for the transcription profile of BC cells and PBMCs showed two shared targets, CCNB2 and
PGK1. Validation with systems biology using reweighted 20 types of cancer signature genes revealed that CCNB2 is the only common target in BC and its related PBMCs, which was further validated by qRT-PCR implying a significant increase in the level of CCNB2
in the BC patients. Conclusion: Results of this study demonstrated that PBMCs are affected by BC cells and CCNB2 may be of value as a diagnostic biomarker for breast cancer. However, verification would require future detailed experimental plans. |
topic |
biomarker breast cancer peripheral blood mononuclear cell systems biology |
url |
https://celljournal.org/journal/article/fulltext/identification-of-ccnb2-as-a-potential-non-invasive-breast-cancer-biomarker-in-peripheral-blood-mononuclear-cells-using-the-systems-biology-approach.pdf |
work_keys_str_mv |
AT rahelehmoradpoor identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach AT hakimehzali identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach AT ahmadgharebaghian identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach AT mohammadesmaeilakbari identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach AT soheilaajdari identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach AT monasalimi identificationofccnb2asapotentialnoninvasivebreastcancerbiomarkerinperipheralbloodmononuclearcellsusingthesystemsbiologyapproach |
_version_ |
1717756052864237568 |