Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core

Functionalization of the catalytic loop of 10–23 DNAzyme with an amino group was performed by incorporation of 7-(3-aminopropyl)-8-aza-7-deaza-2′-deoxyadenosine in different single positions. Among the nine modified positions in the catalytic loop, A9 is the unique position with positive contributio...

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Main Authors:	Qi Wang, Di Zhang, Maosheng Cheng, Junlin He, Keliang Liu
Format:	Article
Language:	English
Published:	Elsevier 2012-02-01
Series:	Acta Pharmaceutica Sinica B
Subjects:	10–23 DNAzyme 8-Aza-7-deaza-2′-deoxyadenosine Amino group Chemical modification
Online Access:	http://www.sciencedirect.com/science/article/pii/S221138351100102X

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spelling	doaj-53ec8020c20f482dadc935952ee251f92020-11-24T23:30:48ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432012-02-0121283110.1016/j.apsb.2011.11.002Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic coreQi Wang0Di Zhang1Maosheng Cheng2Junlin He3Keliang Liu4Beijing Institute of Pharmacology and Toxicology, Beijing 100850, ChinaSchool of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, ChinaBeijing Institute of Pharmacology and Toxicology, Beijing 100850, ChinaBeijing Institute of Pharmacology and Toxicology, Beijing 100850, ChinaFunctionalization of the catalytic loop of 10–23 DNAzyme with an amino group was performed by incorporation of 7-(3-aminopropyl)-8-aza-7-deaza-2′-deoxyadenosine in different single positions. Among the nine modified positions in the catalytic loop, A9 is the unique position with positive contribution by such modification. These results indicated that more efficient deoxyribozymes remain to be explored by introduction of exogenous functional groups in an appropriate position in the catalytic loop of 10–23 DNAzyme, such as the combination of 7-functional group substituted 8-aza-7-deaza-2′-deoxyadenosine analogs and A9 position.http://www.sciencedirect.com/science/article/pii/S221138351100102X10–23 DNAzyme8-Aza-7-deaza-2′-deoxyadenosineAmino groupChemical modification
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Qi Wang Di Zhang Maosheng Cheng Junlin He Keliang Liu
spellingShingle	Qi Wang Di Zhang Maosheng Cheng Junlin He Keliang Liu Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core Acta Pharmaceutica Sinica B 10–23 DNAzyme 8-Aza-7-deaza-2′-deoxyadenosine Amino group Chemical modification
author_facet	Qi Wang Di Zhang Maosheng Cheng Junlin He Keliang Liu
author_sort	Qi Wang
title	Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core
title_short	Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core
title_full	Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core
title_fullStr	Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core
title_full_unstemmed	Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core
title_sort	catalytic cleavage activities of 10–23 dnazyme analogs functionalized with an amino group in its catalytic core
publisher	Elsevier
series	Acta Pharmaceutica Sinica B
issn	2211-3835 2211-3843
publishDate	2012-02-01
description	Functionalization of the catalytic loop of 10–23 DNAzyme with an amino group was performed by incorporation of 7-(3-aminopropyl)-8-aza-7-deaza-2′-deoxyadenosine in different single positions. Among the nine modified positions in the catalytic loop, A9 is the unique position with positive contribution by such modification. These results indicated that more efficient deoxyribozymes remain to be explored by introduction of exogenous functional groups in an appropriate position in the catalytic loop of 10–23 DNAzyme, such as the combination of 7-functional group substituted 8-aza-7-deaza-2′-deoxyadenosine analogs and A9 position.
topic	10–23 DNAzyme 8-Aza-7-deaza-2′-deoxyadenosine Amino group Chemical modification
url	http://www.sciencedirect.com/science/article/pii/S221138351100102X
work_keys_str_mv	AT qiwang catalyticcleavageactivitiesof1023dnazymeanalogsfunctionalizedwithanaminogroupinitscatalyticcore AT dizhang catalyticcleavageactivitiesof1023dnazymeanalogsfunctionalizedwithanaminogroupinitscatalyticcore AT maoshengcheng catalyticcleavageactivitiesof1023dnazymeanalogsfunctionalizedwithanaminogroupinitscatalyticcore AT junlinhe catalyticcleavageactivitiesof1023dnazymeanalogsfunctionalizedwithanaminogroupinitscatalyticcore AT keliangliu catalyticcleavageactivitiesof1023dnazymeanalogsfunctionalizedwithanaminogroupinitscatalyticcore
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Catalytic cleavage activities of 10–23 DNAzyme analogs functionalized with an amino group in its catalytic core

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