Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies
The rise in antibiotic-resistant bacteria and contamination of water bodies is a serious issue that demands immense attention of scientific acumen. Here, we examined the pervasiveness of ESBL producing bacteria in Dal Lake and Wular Lake of Kashmir valley, India. Isolates were screened for antibioti...
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doaj-53fffec75e0543bd97c845b28dbd64152020-11-25T03:27:15ZengElsevierSaudi Journal of Biological Sciences1319-562X2020-10-01271026602668Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodiesInsha Sultan0Asghar Ali1Firdoos A. Gogry2Irfan A. Rather3Jamal S.M. Sabir4Qazi M.R. Haq5Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, IndiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Center of Excellence in Bionanoscience Research, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi Arabia; Center of Excellence in Bionanoscience Research, King Abdulaziz University (KAU), P.O. Box 80141, Jeddah 21589, Saudi ArabiaDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, India; Corresponding author.The rise in antibiotic-resistant bacteria and contamination of water bodies is a serious issue that demands immense attention of scientific acumen. Here, we examined the pervasiveness of ESBL producing bacteria in Dal Lake and Wular Lake of Kashmir valley, India. Isolates were screened for antibiotic, heavy metal resistant elements, and their coexistence with mobile genetic elements. Out of two hundred one isolates screened, thirty-eight were found positive for ESBL production. Antibiotic profiling of ESBL positive isolates with 16 different drugs representing β-lactam or -non-β-lactam, exhibited multidrug resistance phenotype among 55% isolates. Molecular characterization revealed the occurrence of drug resistance determinants blaTEM, AmpC, qnrS, and heavy metal resistance genes (MRGs) merB, merP, merT, silE, silP, silS, and arsC. Furthermore, mobile genetic elements IntI, SulI, ISecp1, TN3, TN21 were also detected. Conjugation assay confirmed the transfer of different ARGs, HMRGs, and mobile elements in recipient Escherichia coli J53 AZR strain. Plasmid incompatibility studies showed blaTEM to be associated with Inc groups B/O, HI1, HI2, I1, N, FIA, and FIB. Co-occurrence of blaTEM, HMRGs, and mobile elements from the aquatic milieu of Kashmir, India has not been reported so far. From this study, the detection of the blaTEM gene in the bacteria Bacillus simplex and Brevibacterium frigoritolerans are found for the first time. Considering all the facts it becomes crucial to conduct studies in natural aquatic environments that could help depict the epidemiological situations in which the resistance mechanism might have clinical relevance.http://www.sciencedirect.com/science/article/pii/S1319562X20302424Antibiotic resistanceblaTEMMobile elementsHMRGsKashmir valleyAquatic environment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Insha Sultan Asghar Ali Firdoos A. Gogry Irfan A. Rather Jamal S.M. Sabir Qazi M.R. Haq |
spellingShingle |
Insha Sultan Asghar Ali Firdoos A. Gogry Irfan A. Rather Jamal S.M. Sabir Qazi M.R. Haq Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies Saudi Journal of Biological Sciences Antibiotic resistance blaTEM Mobile elements HMRGs Kashmir valley Aquatic environment |
author_facet |
Insha Sultan Asghar Ali Firdoos A. Gogry Irfan A. Rather Jamal S.M. Sabir Qazi M.R. Haq |
author_sort |
Insha Sultan |
title |
Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
title_short |
Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
title_full |
Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
title_fullStr |
Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
title_full_unstemmed |
Bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
title_sort |
bacterial isolates harboring antibiotics and heavy-metal resistance genes co-existing with mobile genetic elements in natural aquatic water bodies |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2020-10-01 |
description |
The rise in antibiotic-resistant bacteria and contamination of water bodies is a serious issue that demands immense attention of scientific acumen. Here, we examined the pervasiveness of ESBL producing bacteria in Dal Lake and Wular Lake of Kashmir valley, India. Isolates were screened for antibiotic, heavy metal resistant elements, and their coexistence with mobile genetic elements. Out of two hundred one isolates screened, thirty-eight were found positive for ESBL production. Antibiotic profiling of ESBL positive isolates with 16 different drugs representing β-lactam or -non-β-lactam, exhibited multidrug resistance phenotype among 55% isolates. Molecular characterization revealed the occurrence of drug resistance determinants blaTEM, AmpC, qnrS, and heavy metal resistance genes (MRGs) merB, merP, merT, silE, silP, silS, and arsC. Furthermore, mobile genetic elements IntI, SulI, ISecp1, TN3, TN21 were also detected. Conjugation assay confirmed the transfer of different ARGs, HMRGs, and mobile elements in recipient Escherichia coli J53 AZR strain. Plasmid incompatibility studies showed blaTEM to be associated with Inc groups B/O, HI1, HI2, I1, N, FIA, and FIB. Co-occurrence of blaTEM, HMRGs, and mobile elements from the aquatic milieu of Kashmir, India has not been reported so far. From this study, the detection of the blaTEM gene in the bacteria Bacillus simplex and Brevibacterium frigoritolerans are found for the first time. Considering all the facts it becomes crucial to conduct studies in natural aquatic environments that could help depict the epidemiological situations in which the resistance mechanism might have clinical relevance. |
topic |
Antibiotic resistance blaTEM Mobile elements HMRGs Kashmir valley Aquatic environment |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X20302424 |
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