Fecal Serine Protease Profiling in Inflammatory Bowel Diseases

Serine proteases are extensively known to play key roles in many physiological processes. However, their dysregulation is often associated to several diseases including inflammatory bowel diseases (IBD). Here, we used specific substrates to monitor fecal protease activities in a large cohort of heal...

Full description

Bibliographic Details
Main Authors:	Amin Jablaoui, Aicha Kriaa, Héla Mkaouar, Nizar Akermi, Souha Soussou, Magdalena Wysocka, Dominika Wołoszyn, Ali Amouri, Ali Gargouri, Emmanuelle Maguin, Adam Lesner, Moez Rhimi
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2020-02-01
Series:	Frontiers in Cellular and Infection Microbiology
Subjects:	fecal proteases inflammatory bowel diseases gut microbiota protease profiling serine protease inhibitors Holobiont
Online Access:	https://www.frontiersin.org/article/10.3389/fcimb.2020.00021/full

id	doaj-54277be4bb834245af1a46e4943448a6
record_format	Article
spelling	doaj-54277be4bb834245af1a46e4943448a62020-11-25T00:45:03ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-02-011010.3389/fcimb.2020.00021488664Fecal Serine Protease Profiling in Inflammatory Bowel DiseasesAmin Jablaoui0Aicha Kriaa1Héla Mkaouar2Nizar Akermi3Souha Soussou4Magdalena Wysocka5Dominika Wołoszyn6Ali Amouri7Ali Gargouri8Emmanuelle Maguin9Adam Lesner10Moez Rhimi11Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceFaculty of Chemistry, University of Gdansk, Gdansk, PolandFaculty of Chemistry, University of Gdansk, Gdansk, PolandDepartment of Gastroenterology, Hedi Chaker University Hospital, Sfax, TunisiaLaboratory of Molecular Biology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sfax, TunisiaUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceFaculty of Chemistry, University of Gdansk, Gdansk, PolandUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, Microbiota Interaction with Human and Animal Team (MIHA), Jouy-en-Josas, FranceSerine proteases are extensively known to play key roles in many physiological processes. However, their dysregulation is often associated to several diseases including inflammatory bowel diseases (IBD). Here, we used specific substrates to monitor fecal protease activities in a large cohort of healthy and IBD patients. Of interest, serine protease activity was 10-fold higher in IBD fecal samples compared to healthy controls. Moreover, functional analysis of these fecal proteolytic activities revealed that the most increased activities are trypsin-like, elastase-like and cathepsin G-like. We also show for the first time, an increase of proteinase 3-like activity in these samples compared to controls. Results presented here will guide further investigations to better understand the relevance of these peptidases in IBD.https://www.frontiersin.org/article/10.3389/fcimb.2020.00021/fullfecal proteasesinflammatory bowel diseasesgut microbiotaprotease profilingserine protease inhibitorsHolobiont
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Amin Jablaoui Aicha Kriaa Héla Mkaouar Nizar Akermi Souha Soussou Magdalena Wysocka Dominika Wołoszyn Ali Amouri Ali Gargouri Emmanuelle Maguin Adam Lesner Moez Rhimi
spellingShingle	Amin Jablaoui Aicha Kriaa Héla Mkaouar Nizar Akermi Souha Soussou Magdalena Wysocka Dominika Wołoszyn Ali Amouri Ali Gargouri Emmanuelle Maguin Adam Lesner Moez Rhimi Fecal Serine Protease Profiling in Inflammatory Bowel Diseases Frontiers in Cellular and Infection Microbiology fecal proteases inflammatory bowel diseases gut microbiota protease profiling serine protease inhibitors Holobiont
author_facet	Amin Jablaoui Aicha Kriaa Héla Mkaouar Nizar Akermi Souha Soussou Magdalena Wysocka Dominika Wołoszyn Ali Amouri Ali Gargouri Emmanuelle Maguin Adam Lesner Moez Rhimi
author_sort	Amin Jablaoui
title	Fecal Serine Protease Profiling in Inflammatory Bowel Diseases
title_short	Fecal Serine Protease Profiling in Inflammatory Bowel Diseases
title_full	Fecal Serine Protease Profiling in Inflammatory Bowel Diseases
title_fullStr	Fecal Serine Protease Profiling in Inflammatory Bowel Diseases
title_full_unstemmed	Fecal Serine Protease Profiling in Inflammatory Bowel Diseases
title_sort	fecal serine protease profiling in inflammatory bowel diseases
publisher	Frontiers Media S.A.
series	Frontiers in Cellular and Infection Microbiology
issn	2235-2988
publishDate	2020-02-01
description	Serine proteases are extensively known to play key roles in many physiological processes. However, their dysregulation is often associated to several diseases including inflammatory bowel diseases (IBD). Here, we used specific substrates to monitor fecal protease activities in a large cohort of healthy and IBD patients. Of interest, serine protease activity was 10-fold higher in IBD fecal samples compared to healthy controls. Moreover, functional analysis of these fecal proteolytic activities revealed that the most increased activities are trypsin-like, elastase-like and cathepsin G-like. We also show for the first time, an increase of proteinase 3-like activity in these samples compared to controls. Results presented here will guide further investigations to better understand the relevance of these peptidases in IBD.
topic	fecal proteases inflammatory bowel diseases gut microbiota protease profiling serine protease inhibitors Holobiont
url	https://www.frontiersin.org/article/10.3389/fcimb.2020.00021/full
work_keys_str_mv	AT aminjablaoui fecalserineproteaseprofilingininflammatoryboweldiseases AT aichakriaa fecalserineproteaseprofilingininflammatoryboweldiseases AT helamkaouar fecalserineproteaseprofilingininflammatoryboweldiseases AT nizarakermi fecalserineproteaseprofilingininflammatoryboweldiseases AT souhasoussou fecalserineproteaseprofilingininflammatoryboweldiseases AT magdalenawysocka fecalserineproteaseprofilingininflammatoryboweldiseases AT dominikawołoszyn fecalserineproteaseprofilingininflammatoryboweldiseases AT aliamouri fecalserineproteaseprofilingininflammatoryboweldiseases AT aligargouri fecalserineproteaseprofilingininflammatoryboweldiseases AT emmanuellemaguin fecalserineproteaseprofilingininflammatoryboweldiseases AT adamlesner fecalserineproteaseprofilingininflammatoryboweldiseases AT moezrhimi fecalserineproteaseprofilingininflammatoryboweldiseases
_version_	1725271538952830976

Fecal Serine Protease Profiling in Inflammatory Bowel Diseases

Similar Items