Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2013-01-01
|
Series: | Revista da Sociedade Brasileira de Medicina Tropical |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097&lng=en&tlng=en |
id |
doaj-542bd328f32c426db381339235ea6cd8 |
---|---|
record_format |
Article |
spelling |
doaj-542bd328f32c426db381339235ea6cd82020-11-24T21:07:13ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492013-01-01461979910.1590/0037-868214142013S0037-86822013000100097Production and diagnostic application of recombinant domain III of West Nile envelope protein in BrazilJuliana Helena ChávezVinicius Pinho dos ReisJaqueline Raymondi SilvaHelen Julie LaureJosé Cesar RosaBenedito Antônio Lopes da FonsecaLuiz Tadeu Moraes FigueiredoINTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097&lng=en&tlng=enDiagnosisWNV antibodiesELISA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juliana Helena Chávez Vinicius Pinho dos Reis Jaqueline Raymondi Silva Helen Julie Laure José Cesar Rosa Benedito Antônio Lopes da Fonseca Luiz Tadeu Moraes Figueiredo |
spellingShingle |
Juliana Helena Chávez Vinicius Pinho dos Reis Jaqueline Raymondi Silva Helen Julie Laure José Cesar Rosa Benedito Antônio Lopes da Fonseca Luiz Tadeu Moraes Figueiredo Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil Revista da Sociedade Brasileira de Medicina Tropical Diagnosis WNV antibodies ELISA |
author_facet |
Juliana Helena Chávez Vinicius Pinho dos Reis Jaqueline Raymondi Silva Helen Julie Laure José Cesar Rosa Benedito Antônio Lopes da Fonseca Luiz Tadeu Moraes Figueiredo |
author_sort |
Juliana Helena Chávez |
title |
Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil |
title_short |
Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil |
title_full |
Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil |
title_fullStr |
Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil |
title_full_unstemmed |
Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil |
title_sort |
production and diagnostic application of recombinant domain iii of west nile envelope protein in brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
series |
Revista da Sociedade Brasileira de Medicina Tropical |
issn |
1678-9849 |
publishDate |
2013-01-01 |
description |
INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil. |
topic |
Diagnosis WNV antibodies ELISA |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097&lng=en&tlng=en |
work_keys_str_mv |
AT julianahelenachavez productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT viniciuspinhodosreis productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT jaquelineraymondisilva productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT helenjulielaure productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT josecesarrosa productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT beneditoantoniolopesdafonseca productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil AT luiztadeumoraesfigueiredo productionanddiagnosticapplicationofrecombinantdomainiiiofwestnileenvelopeproteininbrazil |
_version_ |
1716763681166983168 |