Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio)
Background: Cadmium (Cd) intoxication can cause oxidative stress and involve the antioxi-dant defense system in hepatocytes. Administration of vitamin C, chitosan, or a combination of both may prevent Cd-induced oxidative damage. Methods:Cyprinus carpio were distributed into six groups. The contro...
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Arak University of Medical Sciences
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doaj-5439f5be320d4b3bbbb67ab3b33410b12020-11-24T21:35:40ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592015-09-0193013601367Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio)Mahdi Banaee0Behzad Nematdoost Haghi 1Ahmad Noori 2Department of Aquaculture, Behbahan Khatam Alanbia University of Technology, Iran.*Corresponding Author: E-mail: banaee@bkatu.ac.irDepartment of Aquaculture, Behbahan Khatam Alanbia University of Technology, Iran.Department of Aquaculture, Hormozgan University, Bandar Abbas, Iran.Background: Cadmium (Cd) intoxication can cause oxidative stress and involve the antioxi-dant defense system in hepatocytes. Administration of vitamin C, chitosan, or a combination of both may prevent Cd-induced oxidative damage. Methods:Cyprinus carpio were distributed into six groups. The control group received normal feed (Group I). Group II was exposed to 0.2 mg.L-1 cadmium chloride. Group III were fed 1000 mg chitosan per 1 kg feed. Group IV was exposed to 0.2 mg.L-1 cadmium chloride and fed with1000 mg chitosan per 1 kg feed while Group V was exposed to 0.2 mg.L-1 cadmium chlo-ride and was fed with 1000 mg vitamin C per 1 kg feed. Group VI, however, was exposed to 0.2 mg.L-1 cadmium chloride and was fed with 1000 mg vitamin C combined with 1000 mg chi-tosanper 1 kg feed. After 21 days of experiment, activities of hepatic enzymes and oxidative stress biomarkers were evaluated. Results:Exposure to CdCl2 caused a significant increase in malondialdehyde levels and al-tered alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase activities in liver tissues. Hepatic antioxidant capacity, catalase, and Glucose-6-phosphate dehydrogenase activities significantly decreased in fishes exposed to CdCL2 as compared to the control group. Treatment with vitamin C and vitamin C combined with chitosan significantly ameliorated these changes. Conclusion:The present findings suggest that the administration of chitosan failed to restore biochemical parameters to normal levels. However, these findings demonstrate that vitamin C and vitamin C combined with chitosan protect the fish against the toxic effects of CdCl2 on the examined biochemical parameters in liver tissues. http://ijt.arakmu.ac.ir/browse.php?a_id=455&sid=1&slc_lang=enCadmiumChitosanHepatoprotectiveOxidative StressVitamin C. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahdi Banaee Behzad Nematdoost Haghi Ahmad Noori |
spellingShingle |
Mahdi Banaee Behzad Nematdoost Haghi Ahmad Noori Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) Iranian Journal of Toxicology Cadmium Chitosan Hepatoprotective Oxidative Stress Vitamin C. |
author_facet |
Mahdi Banaee Behzad Nematdoost Haghi Ahmad Noori |
author_sort |
Mahdi Banaee |
title |
Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) |
title_short |
Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) |
title_full |
Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) |
title_fullStr |
Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) |
title_full_unstemmed |
Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinuscarpio) |
title_sort |
protective effects of vitamin c and chitosan against cadmium-induced oxidative stress in the liver of common carp (cyprinuscarpio) |
publisher |
Arak University of Medical Sciences |
series |
Iranian Journal of Toxicology |
issn |
2008-2967 2251-9459 |
publishDate |
2015-09-01 |
description |
Background: Cadmium (Cd) intoxication can cause oxidative stress and involve the antioxi-dant defense system in hepatocytes. Administration of vitamin C, chitosan, or a combination of both may prevent Cd-induced oxidative damage.
Methods:Cyprinus carpio were distributed into six groups. The control group received normal feed (Group I). Group II was exposed to 0.2 mg.L-1 cadmium chloride. Group III were fed 1000 mg chitosan per 1 kg feed. Group IV was exposed to 0.2 mg.L-1 cadmium chloride and fed with1000 mg chitosan per 1 kg feed while Group V was exposed to 0.2 mg.L-1 cadmium chlo-ride and was fed with 1000 mg vitamin C per 1 kg feed. Group VI, however, was exposed to 0.2 mg.L-1 cadmium chloride and was fed with 1000 mg vitamin C combined with 1000 mg chi-tosanper 1 kg feed. After 21 days of experiment, activities of hepatic enzymes and oxidative stress biomarkers were evaluated.
Results:Exposure to CdCl2 caused a significant increase in malondialdehyde levels and al-tered alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase activities in liver tissues. Hepatic antioxidant capacity, catalase, and Glucose-6-phosphate dehydrogenase activities significantly decreased in fishes exposed to CdCL2 as compared to the control group. Treatment with vitamin C and vitamin C combined with chitosan significantly ameliorated these changes.
Conclusion:The present findings suggest that the administration of chitosan failed to restore biochemical parameters to normal levels. However, these findings demonstrate that vitamin C and vitamin C combined with chitosan protect the fish against the toxic effects of CdCl2 on the examined biochemical parameters in liver tissues.
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topic |
Cadmium Chitosan Hepatoprotective Oxidative Stress Vitamin C. |
url |
http://ijt.arakmu.ac.ir/browse.php?a_id=455&sid=1&slc_lang=en |
work_keys_str_mv |
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