In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia
The incidence of Acanthamoeba keratitis has been increasing since the previous decades, especially among contact lens users. This infection is majorly caused by the use of ineffective contact lens disinfecting solution. Thus, this study was conducted to evaluate the in vitro effects of multi-purpose...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-04-01
|
Series: | Saudi Journal of Biological Sciences |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X21000309 |
id |
doaj-543b132f75f94750aa3a70353800704b |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rosnani Hanim Mohd Hussain Wan Nur Afiqah Mohamed Kamel Abdul Ghani Naveed Ahmed Khan Ruqaiyyah Siddiqui Tengku Shahrul Anuar |
spellingShingle |
Rosnani Hanim Mohd Hussain Wan Nur Afiqah Mohamed Kamel Abdul Ghani Naveed Ahmed Khan Ruqaiyyah Siddiqui Tengku Shahrul Anuar In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia Saudi Journal of Biological Sciences Acanthamoeba Contact lens Disinfecting solutions Genotype Malaysia Viability |
author_facet |
Rosnani Hanim Mohd Hussain Wan Nur Afiqah Mohamed Kamel Abdul Ghani Naveed Ahmed Khan Ruqaiyyah Siddiqui Tengku Shahrul Anuar |
author_sort |
Rosnani Hanim Mohd Hussain |
title |
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia |
title_short |
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia |
title_full |
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia |
title_fullStr |
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia |
title_full_unstemmed |
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia |
title_sort |
in vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of acanthamoeba genotype t4 in malaysia |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2021-04-01 |
description |
The incidence of Acanthamoeba keratitis has been increasing since the previous decades, especially among contact lens users. This infection is majorly caused by the use of ineffective contact lens disinfecting solution. Thus, this study was conducted to evaluate the in vitro effects of multi-purpose disinfecting solutions (MPDS) against Acanthamoeba trophozoites and cysts. Acanthamoeba genotype T4 isolated from contact lens paraphernalia and an environmental strains were propagated for trophozoite or cyst-containing culture and adjusted in final concentration of 1 × 105 cells/ml. Amoebicidal and cysticidal assays were conducted by incubating trophozoites and cysts with OPTI-FREE® Express®, ReNu® Fresh™, Complete® Multi-Purpose Solution and AVIZOR Unica® Sensitive according to the manufacturer’s minimum recommended disinfectant time (MMRDT) for up to 12 h at 30 ⁰C. Trypan blue hemocytometer-based microscopic counts determined amoebicidal and cysticidal effects. The viability of Acanthamoeba trophozoites and cysts was confirmed by re-inoculated them in the 1.5% non-nutrient agar plates. It was found that none of the MPDS showed amoebicidal and cysticidal effects during the MMRDT. However, OPTI-FREE® Express® demonstrated a significant differences in average cell reduction for both stages within MMRDT. When subjected to 12 h exposure, both OPTI-FREE® Express® and ReNu® Fresh™ led to significant reduction in the number of trophozoite and cyst cells. Notably, Complete® Multi-Purpose Solution and AVIZOR Unica® Sensitive did appreciably improve the solution effectiveness towards trophozoite cells when incubated for 12 h. All MPDS were largely ineffective, with 100% survival of all isolates at MMRDT, while OPTI-FREE® Express® showed limited amoebicidal activity against the contact lens paraphernalia isolate, however, it was more against the environmental strains after 12 h incubation time. The commercially available MPDS employed in this research offered minimal effectiveness against the protozoa despite the contact time. Improvement or development of new solution should consider the adjustment of the appropriate disinfectant concentration, adequate exposure time or the incorporation of novel chemical elements, which are effective against Acanthamoeba for accelerated disinfecting and more reduction of potential exposure of contact lens users to Acanthamoeba keratitis. |
topic |
Acanthamoeba Contact lens Disinfecting solutions Genotype Malaysia Viability |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X21000309 |
work_keys_str_mv |
AT rosnanihanimmohdhussain invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia AT wannurafiqah invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia AT mohamedkamelabdulghani invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia AT naveedahmedkhan invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia AT ruqaiyyahsiddiqui invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia AT tengkushahrulanuar invitroeffectsofmultipurposecontactlensdisinfectingsolutionstowardssurvivabilityofacanthamoebagenotypet4inmalaysia |
_version_ |
1721543317671378944 |
spelling |
doaj-543b132f75f94750aa3a70353800704b2021-04-04T04:18:10ZengElsevierSaudi Journal of Biological Sciences1319-562X2021-04-0128423522359In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in MalaysiaRosnani Hanim Mohd Hussain0Wan Nur Afiqah1Mohamed Kamel Abdul Ghani2Naveed Ahmed Khan3Ruqaiyyah Siddiqui4Tengku Shahrul Anuar5Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, MalaysiaCentre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, MalaysiaProgramme of Biomedical Sciences, School of Diagnostic and Applied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, MalaysiaDepartment of Clinical Sciences, College of Medicine, University of Sharjah, University City, Sharjah, United Arab EmiratesDepartment of Biology, Chemistry and Environmental Sciences, College of Arts and Sciences, American University of Sharjah, University City, Sharjah, United Arab EmiratesCentre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia; Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia; Corresponding author at: Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia. Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, Selangor, Malaysia.The incidence of Acanthamoeba keratitis has been increasing since the previous decades, especially among contact lens users. This infection is majorly caused by the use of ineffective contact lens disinfecting solution. Thus, this study was conducted to evaluate the in vitro effects of multi-purpose disinfecting solutions (MPDS) against Acanthamoeba trophozoites and cysts. Acanthamoeba genotype T4 isolated from contact lens paraphernalia and an environmental strains were propagated for trophozoite or cyst-containing culture and adjusted in final concentration of 1 × 105 cells/ml. Amoebicidal and cysticidal assays were conducted by incubating trophozoites and cysts with OPTI-FREE® Express®, ReNu® Fresh™, Complete® Multi-Purpose Solution and AVIZOR Unica® Sensitive according to the manufacturer’s minimum recommended disinfectant time (MMRDT) for up to 12 h at 30 ⁰C. Trypan blue hemocytometer-based microscopic counts determined amoebicidal and cysticidal effects. The viability of Acanthamoeba trophozoites and cysts was confirmed by re-inoculated them in the 1.5% non-nutrient agar plates. It was found that none of the MPDS showed amoebicidal and cysticidal effects during the MMRDT. However, OPTI-FREE® Express® demonstrated a significant differences in average cell reduction for both stages within MMRDT. When subjected to 12 h exposure, both OPTI-FREE® Express® and ReNu® Fresh™ led to significant reduction in the number of trophozoite and cyst cells. Notably, Complete® Multi-Purpose Solution and AVIZOR Unica® Sensitive did appreciably improve the solution effectiveness towards trophozoite cells when incubated for 12 h. All MPDS were largely ineffective, with 100% survival of all isolates at MMRDT, while OPTI-FREE® Express® showed limited amoebicidal activity against the contact lens paraphernalia isolate, however, it was more against the environmental strains after 12 h incubation time. The commercially available MPDS employed in this research offered minimal effectiveness against the protozoa despite the contact time. Improvement or development of new solution should consider the adjustment of the appropriate disinfectant concentration, adequate exposure time or the incorporation of novel chemical elements, which are effective against Acanthamoeba for accelerated disinfecting and more reduction of potential exposure of contact lens users to Acanthamoeba keratitis.http://www.sciencedirect.com/science/article/pii/S1319562X21000309AcanthamoebaContact lensDisinfecting solutionsGenotypeMalaysiaViability |