Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea
Bovine tuberculosis is a chronic disease impacting both public health and the livestock industry. The interferon (IFN)-γ assay has been introduced as an ancillary test for diagnosing bovine tuberculosis to overcome limitations of the skin test. The objective of this study was to assess the IFN-γ ass...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-04-01
|
Series: | Frontiers in Veterinary Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2020.00222/full |
id |
doaj-54536bd4ae80410689ae0957cb295cca |
---|---|
record_format |
Article |
spelling |
doaj-54536bd4ae80410689ae0957cb295cca2020-11-25T02:13:21ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-04-01710.3389/fvets.2020.00222510232Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South KoreaYun-Ho Jang0Tae-woon Kim1Min Kyu Jeong2Yoon Jeong Seo3Soyoon Ryoo4Chan Ho Park5Sin seok Kang6Young Ju Lee7Soon-Seek Yoon8Jae Myung Kim9Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaFoot and Mouth Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaGangwondo Livestock & Veterinary Service, Chuncheon-si, South KoreaChungcheongbukdo Livestock & Veterinary Service, Chungju-si, South KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, South KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si, South KoreaBovine tuberculosis is a chronic disease impacting both public health and the livestock industry. The interferon (IFN)-γ assay has been introduced as an ancillary test for diagnosing bovine tuberculosis to overcome limitations of the skin test. The objective of this study was to assess the IFN-γ assay in terms of diagnostics and as a nationwide surveillance program in South Korea. From 2012 to 2013, cattle (n = 120) with bovine tuberculosis and cattle (n = 426) from bovine tuberculosis free herds were subjected to the IFN-γ assay to evaluate the sensitivity and specificity of the assay, respectively, depending on various cut-offs (0–3.5). When optical density of the cut-off was 0.1, the sensitivity and specificity were found to be 81.7% (74.7–88.6) and 99.5% (98.9–100.0), respectively. After introducing the IFN-γ assay as part of the national control program, the IFN-γ assay and single caudal fold skin test data were collected from 47 regional veterinary services to compare the results of these two tests. Overall, the agreement between the IFN-γ assay and the single caudal fold skin test (n = 492,068) was 98.2%, and Cohen's kappa value for the two methods was 0.47. Serial and parallel use of the IFN-γ assay and skin test for the bovine tuberculosis control program were compared using samples (n = 91) from cattle confirmed as bovine tuberculosis positive in laboratories from 2014 to 2016. Parallel screening for bTB showed much higher sensitivity (86/91, 94.5%) than the following screening approaches: serial (47.2%, 43/91), single screening using CFT (63.7%, 58/91), or the IFN-γ assay (78.0%, 71/91). These results indicate that the IFN-γ assay and single caudal fold skin test are complementary to each other; therefore, parallel use of these two tests is considered a useful approach to reduce the prevalence of bovine tuberculosis in South Korea.https://www.frontiersin.org/article/10.3389/fvets.2020.00222/fullbovine tuberculosisMycobacterium bovisIFN-γCFTcattle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun-Ho Jang Tae-woon Kim Min Kyu Jeong Yoon Jeong Seo Soyoon Ryoo Chan Ho Park Sin seok Kang Young Ju Lee Soon-Seek Yoon Jae Myung Kim |
spellingShingle |
Yun-Ho Jang Tae-woon Kim Min Kyu Jeong Yoon Jeong Seo Soyoon Ryoo Chan Ho Park Sin seok Kang Young Ju Lee Soon-Seek Yoon Jae Myung Kim Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea Frontiers in Veterinary Science bovine tuberculosis Mycobacterium bovis IFN-γ CFT cattle |
author_facet |
Yun-Ho Jang Tae-woon Kim Min Kyu Jeong Yoon Jeong Seo Soyoon Ryoo Chan Ho Park Sin seok Kang Young Ju Lee Soon-Seek Yoon Jae Myung Kim |
author_sort |
Yun-Ho Jang |
title |
Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea |
title_short |
Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea |
title_full |
Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea |
title_fullStr |
Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea |
title_full_unstemmed |
Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea |
title_sort |
introduction and application of the interferon-γ assay in the national bovine tuberculosis control program in south korea |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-04-01 |
description |
Bovine tuberculosis is a chronic disease impacting both public health and the livestock industry. The interferon (IFN)-γ assay has been introduced as an ancillary test for diagnosing bovine tuberculosis to overcome limitations of the skin test. The objective of this study was to assess the IFN-γ assay in terms of diagnostics and as a nationwide surveillance program in South Korea. From 2012 to 2013, cattle (n = 120) with bovine tuberculosis and cattle (n = 426) from bovine tuberculosis free herds were subjected to the IFN-γ assay to evaluate the sensitivity and specificity of the assay, respectively, depending on various cut-offs (0–3.5). When optical density of the cut-off was 0.1, the sensitivity and specificity were found to be 81.7% (74.7–88.6) and 99.5% (98.9–100.0), respectively. After introducing the IFN-γ assay as part of the national control program, the IFN-γ assay and single caudal fold skin test data were collected from 47 regional veterinary services to compare the results of these two tests. Overall, the agreement between the IFN-γ assay and the single caudal fold skin test (n = 492,068) was 98.2%, and Cohen's kappa value for the two methods was 0.47. Serial and parallel use of the IFN-γ assay and skin test for the bovine tuberculosis control program were compared using samples (n = 91) from cattle confirmed as bovine tuberculosis positive in laboratories from 2014 to 2016. Parallel screening for bTB showed much higher sensitivity (86/91, 94.5%) than the following screening approaches: serial (47.2%, 43/91), single screening using CFT (63.7%, 58/91), or the IFN-γ assay (78.0%, 71/91). These results indicate that the IFN-γ assay and single caudal fold skin test are complementary to each other; therefore, parallel use of these two tests is considered a useful approach to reduce the prevalence of bovine tuberculosis in South Korea. |
topic |
bovine tuberculosis Mycobacterium bovis IFN-γ CFT cattle |
url |
https://www.frontiersin.org/article/10.3389/fvets.2020.00222/full |
work_keys_str_mv |
AT yunhojang introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT taewoonkim introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT minkyujeong introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT yoonjeongseo introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT soyoonryoo introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT chanhopark introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT sinseokkang introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT youngjulee introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT soonseekyoon introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea AT jaemyungkim introductionandapplicationoftheinterferongassayinthenationalbovinetuberculosiscontrolprograminsouthkorea |
_version_ |
1724905800698167296 |