Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC sat...
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2016-09-01
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doaj-5454c76d848e462d808b631ab0b25e162020-11-24T22:34:33ZengElsevierStem Cell Reports2213-67112016-09-017341142410.1016/j.stemcr.2016.08.002Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem CellsNeia Naldaiz-Gastesi0María Goicoechea1Sonia Alonso-Martín2Ana Aiastui3Macarena López-Mayorga4Paula García-Belda5Jaione Lacalle6Carlos San José7Marcos J. Araúzo-Bravo8Lidwine Trouilh9Véronique Anton-Leberre10Diego Herrero11Ander Matheu12Antonio Bernad13José Manuel García-Verdugo14Jaime J. Carvajal15Frédéric Relaix16Adolfo Lopez de Munain17Patricia García-Parra18Ander Izeta19Tissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, SpainNeuroscience Area, Instituto Biodonostia, San Sebastián 20014, SpainINSERM U955-E10, Université Paris Est, Faculté de Médicine, IMRB U955-E10, Creteil 94000, FranceNeuroscience Area, Instituto Biodonostia, San Sebastián 20014, SpainMolecular Embryology Team, Centro Andaluz de Biología del Desarrollo, Sevilla 41013, SpainCIBERNED, Instituto de Salud Carlos III, Madrid 28029, SpainTissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, SpainAnimal Facility and Experimental Surgery, Instituto Biodonostia, San Sebastián 20014, SpainComputational Biology and Systems Biomedicine, Instituto Biodonostia, San Sebastián 20014, SpainINSA, UPS, INP, LISBP, Université de Toulouse, 31077 Toulouse, FranceINSA, UPS, INP, LISBP, Université de Toulouse, 31077 Toulouse, FranceImmunology and Oncology Department, Spanish National Center for Biotechnology (CNB-CSIC), Madrid 28049, SpainIKERBASQUE, Basque Foundation for Science, Bilbao 48013, SpainImmunology and Oncology Department, Spanish National Center for Biotechnology (CNB-CSIC), Madrid 28049, SpainCIBERNED, Instituto de Salud Carlos III, Madrid 28029, SpainMolecular Embryology Team, Centro Andaluz de Biología del Desarrollo, Sevilla 41013, SpainINSERM U955-E10, Université Paris Est, Faculté de Médicine, IMRB U955-E10, Creteil 94000, FranceNeuroscience Area, Instituto Biodonostia, San Sebastián 20014, SpainTissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, SpainTissue Engineering Laboratory, Bioengineering Area, Instituto Biodonostia, San Sebastián 20014, SpainThe dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5+, Pax3/Pax7+ cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment.http://www.sciencedirect.com/science/article/pii/S2213671116301527 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neia Naldaiz-Gastesi María Goicoechea Sonia Alonso-Martín Ana Aiastui Macarena López-Mayorga Paula García-Belda Jaione Lacalle Carlos San José Marcos J. Araúzo-Bravo Lidwine Trouilh Véronique Anton-Leberre Diego Herrero Ander Matheu Antonio Bernad José Manuel García-Verdugo Jaime J. Carvajal Frédéric Relaix Adolfo Lopez de Munain Patricia García-Parra Ander Izeta |
spellingShingle |
Neia Naldaiz-Gastesi María Goicoechea Sonia Alonso-Martín Ana Aiastui Macarena López-Mayorga Paula García-Belda Jaione Lacalle Carlos San José Marcos J. Araúzo-Bravo Lidwine Trouilh Véronique Anton-Leberre Diego Herrero Ander Matheu Antonio Bernad José Manuel García-Verdugo Jaime J. Carvajal Frédéric Relaix Adolfo Lopez de Munain Patricia García-Parra Ander Izeta Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells Stem Cell Reports |
author_facet |
Neia Naldaiz-Gastesi María Goicoechea Sonia Alonso-Martín Ana Aiastui Macarena López-Mayorga Paula García-Belda Jaione Lacalle Carlos San José Marcos J. Araúzo-Bravo Lidwine Trouilh Véronique Anton-Leberre Diego Herrero Ander Matheu Antonio Bernad José Manuel García-Verdugo Jaime J. Carvajal Frédéric Relaix Adolfo Lopez de Munain Patricia García-Parra Ander Izeta |
author_sort |
Neia Naldaiz-Gastesi |
title |
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_short |
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_full |
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_fullStr |
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_full_unstemmed |
Identification and Characterization of the Dermal Panniculus Carnosus Muscle Stem Cells |
title_sort |
identification and characterization of the dermal panniculus carnosus muscle stem cells |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2016-09-01 |
description |
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with regard to developmental origin and purported function. Lineage tracing shows that they originate in Myf5+, Pax3/Pax7+ cell populations. Skin and muscle wounding increased PC myofiber turnover, with the satellite cell progeny being involved in muscle regeneration but with no detectable contribution to the wound-bed myofibroblasts. Since hematopoietic stem cells fuse to PC myofibers in the absence of injury, we also studied the contribution of bone marrow-derived cells to the PC satellite cell compartment, demonstrating that cells of donor origin are capable of repopulating the PC muscle stem cell niche after irradiation and bone marrow transplantation but may not fully acquire the relevant myogenic commitment. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671116301527 |
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