Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET
Abstract Objective Melatonin has been shown to increase brown adipose tissue (BAT) mass, which can lead to important metabolic effects, such as bodyweight reduction and glycemic improvement. However, BAT mass can only be measured invasively and. The gold standard for non-invasive measurement of BAT...
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doaj-54671d58f4824dc081306544aac1c36e2020-11-25T03:21:42ZengBMCDiabetology & Metabolic Syndrome1758-59962020-09-011211710.1186/s13098-020-00589-1Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PETBruno Halpern0Marcio C. Mancini1Caroline Mendes2Camila Maria Longo Machado3Silvana Prando4Marcelo Tatit Sapienza5Carlos Alberto Buchpiguel6Fernanda Gaspar do Amaral7José Cipolla-Neto8Department of Endocrinology and Metabolism, Hospital das Clinicas da Faculdade de Medicina de São PauloDepartment of Endocrinology and Metabolism, Hospital das Clinicas da Faculdade de Medicina de São PauloDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, Universidade de São PauloNuclear Medicine Institute, Hospital das Clinicas da Faculdade de Medicina de São PauloNuclear Medicine Institute, Hospital das Clinicas da Faculdade de Medicina de São PauloNuclear Medicine Institute, Hospital das Clinicas da Faculdade de Medicina de São PauloNuclear Medicine Institute, Hospital das Clinicas da Faculdade de Medicina de São PauloDepartment of Physiology, Federal University of São PauloDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, Universidade de São PauloAbstract Objective Melatonin has been shown to increase brown adipose tissue (BAT) mass, which can lead to important metabolic effects, such as bodyweight reduction and glycemic improvement. However, BAT mass can only be measured invasively and. The gold standard for non-invasive measurement of BAT activity is positron emission tomography with 2-deoxy-2-[fluorine-18] fluoro-d-glucose (18F-FDG PET). There is no study, to our knowledge, that has evaluated if melatonin influences BAT activity, measured by this imaging technique in animals. Methods Three experimental groups of Wistar rats (control, pinealectomy, and pinealectomy replaced with melatonin) had an 18F-FDG PET performed at room temperature and after acute cold exposure. The ratio of increased BAT activity after cold exposure/room temperature was called “acute thermogenic capacity” (ATC) We also measured UCP-1 mRNA expression to correlate with the 18F-FDG PET results. Results Pinealectomy led to reduced acute thermogenic capacity, compared with the other groups, as well as reduced UCP1 mRNA expression. Conclusion Melatonin deficiency impairs BAT response when exposed to acute cold exposure. These results can lead to future studies of the influence of melatonin on BAT, in animals and humans, without needing an invasive evaluation of BAT.http://link.springer.com/article/10.1186/s13098-020-00589-1Brown adipose tissueMelatoninObesityCircadian rhythmsFDG-PETThermogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bruno Halpern Marcio C. Mancini Caroline Mendes Camila Maria Longo Machado Silvana Prando Marcelo Tatit Sapienza Carlos Alberto Buchpiguel Fernanda Gaspar do Amaral José Cipolla-Neto |
spellingShingle |
Bruno Halpern Marcio C. Mancini Caroline Mendes Camila Maria Longo Machado Silvana Prando Marcelo Tatit Sapienza Carlos Alberto Buchpiguel Fernanda Gaspar do Amaral José Cipolla-Neto Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET Diabetology & Metabolic Syndrome Brown adipose tissue Melatonin Obesity Circadian rhythms FDG-PET Thermogenesis |
author_facet |
Bruno Halpern Marcio C. Mancini Caroline Mendes Camila Maria Longo Machado Silvana Prando Marcelo Tatit Sapienza Carlos Alberto Buchpiguel Fernanda Gaspar do Amaral José Cipolla-Neto |
author_sort |
Bruno Halpern |
title |
Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET |
title_short |
Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET |
title_full |
Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET |
title_fullStr |
Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET |
title_full_unstemmed |
Melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18F-FDG PET |
title_sort |
melatonin deficiency decreases brown adipose tissue acute thermogenic capacity of in rats measured by 18f-fdg pet |
publisher |
BMC |
series |
Diabetology & Metabolic Syndrome |
issn |
1758-5996 |
publishDate |
2020-09-01 |
description |
Abstract Objective Melatonin has been shown to increase brown adipose tissue (BAT) mass, which can lead to important metabolic effects, such as bodyweight reduction and glycemic improvement. However, BAT mass can only be measured invasively and. The gold standard for non-invasive measurement of BAT activity is positron emission tomography with 2-deoxy-2-[fluorine-18] fluoro-d-glucose (18F-FDG PET). There is no study, to our knowledge, that has evaluated if melatonin influences BAT activity, measured by this imaging technique in animals. Methods Three experimental groups of Wistar rats (control, pinealectomy, and pinealectomy replaced with melatonin) had an 18F-FDG PET performed at room temperature and after acute cold exposure. The ratio of increased BAT activity after cold exposure/room temperature was called “acute thermogenic capacity” (ATC) We also measured UCP-1 mRNA expression to correlate with the 18F-FDG PET results. Results Pinealectomy led to reduced acute thermogenic capacity, compared with the other groups, as well as reduced UCP1 mRNA expression. Conclusion Melatonin deficiency impairs BAT response when exposed to acute cold exposure. These results can lead to future studies of the influence of melatonin on BAT, in animals and humans, without needing an invasive evaluation of BAT. |
topic |
Brown adipose tissue Melatonin Obesity Circadian rhythms FDG-PET Thermogenesis |
url |
http://link.springer.com/article/10.1186/s13098-020-00589-1 |
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