Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats
This study aimed to evaluate the antitumor activity of platinum nanoparticles compared with cis -platin both in vitro and in vivo in the treatment of hepatocellular carcinoma induced in rats. The treatment efficacy of platinum nanoparticles was evaluated by measuring antioxidant activities against o...
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doaj-546ab20e50424e7489d2376a387babd72021-05-02T18:13:59ZengIOS PressTumor Biology1423-03802017-07-013910.1177/1010428317717259Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in ratsAmina Medhat0Somaya Mansour1Sawsan El-sonbaty2Eman Kandil3Mustafa Mahmoud4Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, EgyptNational Center for Radiation, Research and Technology, Atomic Energy Authority, Cairo, EgyptNational Center for Radiation, Research and Technology, Atomic Energy Authority, Cairo, EgyptDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo, EgyptDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo, EgyptThis study aimed to evaluate the antitumor activity of platinum nanoparticles compared with cis -platin both in vitro and in vivo in the treatment of hepatocellular carcinoma induced in rats. The treatment efficacy of platinum nanoparticles was evaluated by measuring antioxidant activities against oxidative stress caused by diethylnitrosamine in liver tissue. The measurements included reduced glutathione content and superoxide dismutase activity, as well as malondialdehyde level. Liver function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein, caspase-3, and cytochrome c in liver tissue. Total RNA extraction from liver tissue samples was also done for the relative quantification of B-cell lymphoma 2, matrix metallopeptidase 9, and tumor protein p53 genes. Histopathological examination was also performed for liver tissue. Results showed that platinum nanoparticles are more potent than cis -platin in treatment of hepatocellular carcinoma induced by diethylnitrosamine in rats as it ameliorated the investigated parameters toward normal control animals. These findings were well appreciated with histopathological studies of diethylnitrosamine group treated with platinum nanoparticles, suggesting that platinum nanoparticles can serve as a good therapeutic agent for the treatment of hepatocellular carcinoma which should attract further studies.https://doi.org/10.1177/1010428317717259 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amina Medhat Somaya Mansour Sawsan El-sonbaty Eman Kandil Mustafa Mahmoud |
spellingShingle |
Amina Medhat Somaya Mansour Sawsan El-sonbaty Eman Kandil Mustafa Mahmoud Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats Tumor Biology |
author_facet |
Amina Medhat Somaya Mansour Sawsan El-sonbaty Eman Kandil Mustafa Mahmoud |
author_sort |
Amina Medhat |
title |
Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
title_short |
Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
title_full |
Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
title_fullStr |
Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
title_full_unstemmed |
Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
title_sort |
evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats |
publisher |
IOS Press |
series |
Tumor Biology |
issn |
1423-0380 |
publishDate |
2017-07-01 |
description |
This study aimed to evaluate the antitumor activity of platinum nanoparticles compared with cis -platin both in vitro and in vivo in the treatment of hepatocellular carcinoma induced in rats. The treatment efficacy of platinum nanoparticles was evaluated by measuring antioxidant activities against oxidative stress caused by diethylnitrosamine in liver tissue. The measurements included reduced glutathione content and superoxide dismutase activity, as well as malondialdehyde level. Liver function tests were also determined, in addition to the evaluation of serum alpha-fetoprotein, caspase-3, and cytochrome c in liver tissue. Total RNA extraction from liver tissue samples was also done for the relative quantification of B-cell lymphoma 2, matrix metallopeptidase 9, and tumor protein p53 genes. Histopathological examination was also performed for liver tissue. Results showed that platinum nanoparticles are more potent than cis -platin in treatment of hepatocellular carcinoma induced by diethylnitrosamine in rats as it ameliorated the investigated parameters toward normal control animals. These findings were well appreciated with histopathological studies of diethylnitrosamine group treated with platinum nanoparticles, suggesting that platinum nanoparticles can serve as a good therapeutic agent for the treatment of hepatocellular carcinoma which should attract further studies. |
url |
https://doi.org/10.1177/1010428317717259 |
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