Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products
<p>Abstract</p> <p>Background</p> <p>Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely af...
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doaj-546f2d4f4e7a49e8b1ec9b91be14a4312021-08-02T06:51:20ZengBMCChemistry Central Journal1752-153X2012-08-01619310.1186/1752-153X-6-93Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic productsMusharraf SyedShoaib MuhammadKumar DileepNajam-ul-Haq Muhammad<p>Abstract</p> <p>Background</p> <p>Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely affect human health and environment. Hence a careful selection of environment friendly household product is required that must contain exactly the label claimed pyrethroids amount.</p> <p>Results</p> <p>A sensitive and robust TLC-densitometric method for simultaneous quantification of commonly used synthetic pyrethroids including esbiothrin, alpha-cypermethrin and <it>cis</it>/<it>trans</it> permethrin in agricultural and domestic products has been developed and validated. TLC aluminum sheets, precoated with 0.2 mm thick layer of silica gel 60 F-254, were used for chromatographic process. Densitometric analysis of chromatoplates was carried out in absorbance mode at corresponding λ<sub>max</sub> of each pyrethroid. Equally valid common mobile phase for all pyrethroids consisted of hexane-dichloromethane-ethylacetate-formic acid (8:1.5:0.4:0.1 v/v/v/v) which provided sharp and symmetrical peaks of esbiothrin, alpha-cypermethrin, <it>trans</it>-permethrin and <it>cis</it>-permethrin, at <it>R</it><sub>f</sub> 0.31, 0.53, 0.6 and 0.65, respectively. Linear regression data for respective calibration curves showed a good linearity for all pyrethroids with <it>r</it> = 0.991-0.996. Limits of detection (LOD) and limits of quantification (LOQ) for all pyrethroids were found in the range of 1.6-2.8 and 4.9-8.5 ng/spot, respectively.</p> <p>Conclusions</p> <p>The developed method is applicable for separating the mixture of pyrethroids and at the same time, it is also valid for separating their isomers. The method is reproducible, precise and accurate for the quantitative determination of pyrethroids in agricultural and domestic products.</p> http://journal.chemistrycentral.com/content/6/1/93Pyrethroid insecticidesAgricultural and domestic productsTLC-densitometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Musharraf Syed Shoaib Muhammad Kumar Dileep Najam-ul-Haq Muhammad |
spellingShingle |
Musharraf Syed Shoaib Muhammad Kumar Dileep Najam-ul-Haq Muhammad Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products Chemistry Central Journal Pyrethroid insecticides Agricultural and domestic products TLC-densitometry |
author_facet |
Musharraf Syed Shoaib Muhammad Kumar Dileep Najam-ul-Haq Muhammad |
author_sort |
Musharraf Syed |
title |
Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_short |
Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_full |
Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_fullStr |
Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_full_unstemmed |
Validated TLC-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
title_sort |
validated tlc-densitometry method for the simultaneous analysis of pyrethroid insecticides in agricultural and domestic products |
publisher |
BMC |
series |
Chemistry Central Journal |
issn |
1752-153X |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Pyrethroids are widely used for the control of pests and insects, as pyrethroids are believed to pose little risk to human health and environment. However, exposure to the pyrethroids exceeding the label directions might adversely affect human health and environment. Hence a careful selection of environment friendly household product is required that must contain exactly the label claimed pyrethroids amount.</p> <p>Results</p> <p>A sensitive and robust TLC-densitometric method for simultaneous quantification of commonly used synthetic pyrethroids including esbiothrin, alpha-cypermethrin and <it>cis</it>/<it>trans</it> permethrin in agricultural and domestic products has been developed and validated. TLC aluminum sheets, precoated with 0.2 mm thick layer of silica gel 60 F-254, were used for chromatographic process. Densitometric analysis of chromatoplates was carried out in absorbance mode at corresponding λ<sub>max</sub> of each pyrethroid. Equally valid common mobile phase for all pyrethroids consisted of hexane-dichloromethane-ethylacetate-formic acid (8:1.5:0.4:0.1 v/v/v/v) which provided sharp and symmetrical peaks of esbiothrin, alpha-cypermethrin, <it>trans</it>-permethrin and <it>cis</it>-permethrin, at <it>R</it><sub>f</sub> 0.31, 0.53, 0.6 and 0.65, respectively. Linear regression data for respective calibration curves showed a good linearity for all pyrethroids with <it>r</it> = 0.991-0.996. Limits of detection (LOD) and limits of quantification (LOQ) for all pyrethroids were found in the range of 1.6-2.8 and 4.9-8.5 ng/spot, respectively.</p> <p>Conclusions</p> <p>The developed method is applicable for separating the mixture of pyrethroids and at the same time, it is also valid for separating their isomers. The method is reproducible, precise and accurate for the quantitative determination of pyrethroids in agricultural and domestic products.</p> |
topic |
Pyrethroid insecticides Agricultural and domestic products TLC-densitometry |
url |
http://journal.chemistrycentral.com/content/6/1/93 |
work_keys_str_mv |
AT musharrafsyed validatedtlcdensitometrymethodforthesimultaneousanalysisofpyrethroidinsecticidesinagriculturalanddomesticproducts AT shoaibmuhammad validatedtlcdensitometrymethodforthesimultaneousanalysisofpyrethroidinsecticidesinagriculturalanddomesticproducts AT kumardileep validatedtlcdensitometrymethodforthesimultaneousanalysisofpyrethroidinsecticidesinagriculturalanddomesticproducts AT najamulhaqmuhammad validatedtlcdensitometrymethodforthesimultaneousanalysisofpyrethroidinsecticidesinagriculturalanddomesticproducts |
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