IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.
In this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific I...
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doaj-5476bf1fef48402eafedb4a555b17f512021-04-21T17:24:38ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-10-01910e100366210.1371/journal.ppat.1003662IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection.William G C HorsnellMatthew G DarbyJennifer C HovingNatalie NieuwenhuizenHenry J McSorleyHlumani NdlovuSaeeda BobatMatti KimbergFrank KirsteinAnthony J CutlerBenjamin DewalsAdam F CunninghamFrank BrombacherIn this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα⁻/⁻ mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4⁺ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88⁻/⁻ B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4⁺ T cell-mediated protective immunity against N. brasiliensis infection.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204255/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher |
spellingShingle |
William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. PLoS Pathogens |
author_facet |
William G C Horsnell Matthew G Darby Jennifer C Hoving Natalie Nieuwenhuizen Henry J McSorley Hlumani Ndlovu Saeeda Bobat Matti Kimberg Frank Kirstein Anthony J Cutler Benjamin Dewals Adam F Cunningham Frank Brombacher |
author_sort |
William G C Horsnell |
title |
IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. |
title_short |
IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. |
title_full |
IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. |
title_fullStr |
IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. |
title_full_unstemmed |
IL-4Rα-associated antigen processing by B cells promotes immunity in Nippostrongylus brasiliensis infection. |
title_sort |
il-4rα-associated antigen processing by b cells promotes immunity in nippostrongylus brasiliensis infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2013-10-01 |
description |
In this study, B cell function in protective T(H)2 immunity against N. brasiliensis infection was investigated. Protection against secondary infection depended on IL-4Rα and IL-13; but not IL-4. Protection did not associate with parasite specific antibody responses. Re-infection of B cell-specific IL-4Rα⁻/⁻ mice resulted in increased worm burdens compared to control mice, despite their equivalent capacity to control primary infection. Impaired protection correlated with reduced lymphocyte IL-13 production and B cell MHC class II and CD86 surface expression. Adoptive transfer of in vivo N. brasiliensis primed IL-4Rα expressing B cells into naïve BALB/c mice, but not IL-4Rα or IL-13 deficient B cells, conferred protection against primary N. brasiliensis infection. This protection required MHC class II compatibility on B cells suggesting cognate interactions by B cells with CD4⁺ T cells were important to co-ordinate immunity. Furthermore, the rapid nature of these protective effects by B cells suggested non-BCR mediated mechanisms, such as via Toll Like Receptors, was involved, and this was supported by transfer experiments using antigen pulsed Myd88⁻/⁻ B cells. These data suggest TLR dependent antigen processing by IL-4Rα-responsive B cells producing IL-13 contribute significantly to CD4⁺ T cell-mediated protective immunity against N. brasiliensis infection. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204255/pdf/?tool=EBI |
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