A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control.
How cells control the overall size and growth of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size proportional to cellular size, resulting in a constant ratio between nuclear and cellular volumes (N/C ratio). We have conducted...
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2017-05-01
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doaj-54803857d3d34537b796bcc0c8f3b0d12020-11-25T01:53:33ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-05-01135e100676710.1371/journal.pgen.1006767A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control.Kazunori KumeHelena CantwellFrank R NeumannAndrew W JonesAmbrosius P SnijdersPaul NurseHow cells control the overall size and growth of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size proportional to cellular size, resulting in a constant ratio between nuclear and cellular volumes (N/C ratio). We have conducted a genome-wide visual screen of a fission yeast gene deletion collection for viable mutants altered in their N/C ratio, and have found that defects in both nucleocytoplasmic mRNA transport and lipid synthesis alter the N/C ratio. Perturbing nuclear mRNA export results in accumulation of both mRNA and protein within the nucleus, and leads to an increase in the N/C ratio which is dependent on new membrane synthesis. Disruption of lipid synthesis dysregulates nuclear membrane growth and results in an enlarged N/C ratio. We propose that both properly regulated nucleocytoplasmic transport and nuclear membrane growth are central to the control of nuclear growth and size.http://europepmc.org/articles/PMC5436639?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kazunori Kume Helena Cantwell Frank R Neumann Andrew W Jones Ambrosius P Snijders Paul Nurse |
spellingShingle |
Kazunori Kume Helena Cantwell Frank R Neumann Andrew W Jones Ambrosius P Snijders Paul Nurse A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. PLoS Genetics |
author_facet |
Kazunori Kume Helena Cantwell Frank R Neumann Andrew W Jones Ambrosius P Snijders Paul Nurse |
author_sort |
Kazunori Kume |
title |
A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
title_short |
A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
title_full |
A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
title_fullStr |
A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
title_full_unstemmed |
A systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
title_sort |
systematic genomic screen implicates nucleocytoplasmic transport and membrane growth in nuclear size control. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2017-05-01 |
description |
How cells control the overall size and growth of membrane-bound organelles is an important unanswered question of cell biology. Fission yeast cells maintain a nuclear size proportional to cellular size, resulting in a constant ratio between nuclear and cellular volumes (N/C ratio). We have conducted a genome-wide visual screen of a fission yeast gene deletion collection for viable mutants altered in their N/C ratio, and have found that defects in both nucleocytoplasmic mRNA transport and lipid synthesis alter the N/C ratio. Perturbing nuclear mRNA export results in accumulation of both mRNA and protein within the nucleus, and leads to an increase in the N/C ratio which is dependent on new membrane synthesis. Disruption of lipid synthesis dysregulates nuclear membrane growth and results in an enlarged N/C ratio. We propose that both properly regulated nucleocytoplasmic transport and nuclear membrane growth are central to the control of nuclear growth and size. |
url |
http://europepmc.org/articles/PMC5436639?pdf=render |
work_keys_str_mv |
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