Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus
Abstract Objective Survivors of Ebola virus disease (EVD) are at risk of developing blinding intraocular inflammation—or uveitis—which is associated with retinal pigment epithelial (RPE) scarring and persistence of live Zaire ebolavirus (EBOV) within the eye. As part of a large research project aime...
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doaj-548230fcafd648e380b8557b5a29c0e42020-11-25T02:49:52ZengBMCBMC Research Notes1756-05002019-10-011211710.1186/s13104-019-4671-8Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirusGenevieve F. Oliver0Ayla V. Orang1Binoy Appukuttan2Shashikanth Marri3Michael Z. Michael4Glenn A. Marsh5Justine R. Smith6Flinders University College of Medicine and Public HealthFlinders University College of Medicine and Public HealthFlinders University College of Medicine and Public HealthFlinders University College of Medicine and Public HealthFlinders University College of Medicine and Public HealthHealth and Biosecurity, Commonwealth Scientific and Industrial Research OrganisationFlinders University College of Medicine and Public HealthAbstract Objective Survivors of Ebola virus disease (EVD) are at risk of developing blinding intraocular inflammation—or uveitis—which is associated with retinal pigment epithelial (RPE) scarring and persistence of live Zaire ebolavirus (EBOV) within the eye. As part of a large research project aimed at defining the human RPE cell response to being infected with EBOV, this work focused on the microRNAs (miRNAs) associated with the infection. Results Using RNA-sequencing, we detected 13 highly induced and 2 highly repressed human miRNAs in human ARPE-19 RPE cells infected with EBOV, including hsa-miR-1307-5p, hsa-miR-29b-3p and hsa-miR-33a-5p (up-regulated), and hsa-miR-3074-3p and hsa-miR-27b-5p (down-regulated). EBOV-miR-1-5p was also found in infected RPE cells. Through computational identification of putative miRNA targets, we predicted a broad range of regulatory activities, including effects on innate and adaptive immune responses, cellular metabolism, cell cycle progression, apoptosis and autophagy. The most highly-connected molecule in the miR-target network was leucine-rich repeat kinase 2, which is involved in neuroinflammation and lysosomal processing. Our findings should stimulate new studies on the impact of miRNA changes in EBOV-infected RPE cells to further understanding of intraocular viral persistence and the pathogenesis of uveitis in EVD survivors.http://link.springer.com/article/10.1186/s13104-019-4671-8EbolaFilovirusmicroRNARetinaRetinal pigment epitheliumUveitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Genevieve F. Oliver Ayla V. Orang Binoy Appukuttan Shashikanth Marri Michael Z. Michael Glenn A. Marsh Justine R. Smith |
spellingShingle |
Genevieve F. Oliver Ayla V. Orang Binoy Appukuttan Shashikanth Marri Michael Z. Michael Glenn A. Marsh Justine R. Smith Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus BMC Research Notes Ebola Filovirus microRNA Retina Retinal pigment epithelium Uveitis |
author_facet |
Genevieve F. Oliver Ayla V. Orang Binoy Appukuttan Shashikanth Marri Michael Z. Michael Glenn A. Marsh Justine R. Smith |
author_sort |
Genevieve F. Oliver |
title |
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus |
title_short |
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus |
title_full |
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus |
title_fullStr |
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus |
title_full_unstemmed |
Expression of microRNA in human retinal pigment epithelial cells following infection with Zaire ebolavirus |
title_sort |
expression of microrna in human retinal pigment epithelial cells following infection with zaire ebolavirus |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2019-10-01 |
description |
Abstract Objective Survivors of Ebola virus disease (EVD) are at risk of developing blinding intraocular inflammation—or uveitis—which is associated with retinal pigment epithelial (RPE) scarring and persistence of live Zaire ebolavirus (EBOV) within the eye. As part of a large research project aimed at defining the human RPE cell response to being infected with EBOV, this work focused on the microRNAs (miRNAs) associated with the infection. Results Using RNA-sequencing, we detected 13 highly induced and 2 highly repressed human miRNAs in human ARPE-19 RPE cells infected with EBOV, including hsa-miR-1307-5p, hsa-miR-29b-3p and hsa-miR-33a-5p (up-regulated), and hsa-miR-3074-3p and hsa-miR-27b-5p (down-regulated). EBOV-miR-1-5p was also found in infected RPE cells. Through computational identification of putative miRNA targets, we predicted a broad range of regulatory activities, including effects on innate and adaptive immune responses, cellular metabolism, cell cycle progression, apoptosis and autophagy. The most highly-connected molecule in the miR-target network was leucine-rich repeat kinase 2, which is involved in neuroinflammation and lysosomal processing. Our findings should stimulate new studies on the impact of miRNA changes in EBOV-infected RPE cells to further understanding of intraocular viral persistence and the pathogenesis of uveitis in EVD survivors. |
topic |
Ebola Filovirus microRNA Retina Retinal pigment epithelium Uveitis |
url |
http://link.springer.com/article/10.1186/s13104-019-4671-8 |
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