Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina
Thin anodic porous alumina (tAPA) was fabricated from a 500 nm thick aluminum (Al) layer coated on silicon wafers, through single-step anodization performed in a Teflon electrochemical cell in 0.4 M aqueous phosphoric acid at 110 V. Post-fabrication etching in the same acid allowed obtaining tAPA su...
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doaj-5495797bbdee4826b7539f97f4edf1f92020-11-25T01:26:13ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862017-01-0181748110.3762/bjnano.8.82190-4286-8-8Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous aluminaMarco Salerno0Amirreza Shayganpour1Barbara Salis2Silvia Dante3Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, ItalyDepartment of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, ItalyDepartment of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, ItalyDepartment of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, I-16163 Genova, ItalyThin anodic porous alumina (tAPA) was fabricated from a 500 nm thick aluminum (Al) layer coated on silicon wafers, through single-step anodization performed in a Teflon electrochemical cell in 0.4 M aqueous phosphoric acid at 110 V. Post-fabrication etching in the same acid allowed obtaining tAPA surfaces with ≈160 nm pore diameter and ≈80 nm corresponding wall thickness to be prepared. The tAPA surfaces were made SERS-active by coating with a thin (≈25 nm) gold (Au) layer. The as obtained tAPA–Au substrates were incubated first with different thiols, namely mercaptobenzoic acid (MbA) and aminothiol (AT), and then with phospholipid vesicles of different composition to form a supported lipid bilayer (SLB). At each step, the SERS substrate functionality was assessed, demonstrating acceptable enhancement (≥100×). The chemisorption of thiols during the first step and the formation of SLB from the vesicles during the second step, were independently monitored by using a quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The SLB membranes represent a simplified model system of the living cells membranes, which makes the successful observation of SERS on these films promising in view of the use of tAPA–Au substrates as a platform for the development of surface-enhanced Raman spectroscopy (SERS) biosensors on living cells. In the future, these tAPA–Au-SLB substrates will be investigated also for drug delivery of bioactive agents from the APA pores.https://doi.org/10.3762/bjnano.8.8anodic porous aluminaSERSnanoporessupported lipid bilayersthiols |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco Salerno Amirreza Shayganpour Barbara Salis Silvia Dante |
spellingShingle |
Marco Salerno Amirreza Shayganpour Barbara Salis Silvia Dante Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina Beilstein Journal of Nanotechnology anodic porous alumina SERS nanopores supported lipid bilayers thiols |
author_facet |
Marco Salerno Amirreza Shayganpour Barbara Salis Silvia Dante |
author_sort |
Marco Salerno |
title |
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
title_short |
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
title_full |
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
title_fullStr |
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
title_full_unstemmed |
Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
title_sort |
surface-enhanced raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2017-01-01 |
description |
Thin anodic porous alumina (tAPA) was fabricated from a 500 nm thick aluminum (Al) layer coated on silicon wafers, through single-step anodization performed in a Teflon electrochemical cell in 0.4 M aqueous phosphoric acid at 110 V. Post-fabrication etching in the same acid allowed obtaining tAPA surfaces with ≈160 nm pore diameter and ≈80 nm corresponding wall thickness to be prepared. The tAPA surfaces were made SERS-active by coating with a thin (≈25 nm) gold (Au) layer. The as obtained tAPA–Au substrates were incubated first with different thiols, namely mercaptobenzoic acid (MbA) and aminothiol (AT), and then with phospholipid vesicles of different composition to form a supported lipid bilayer (SLB). At each step, the SERS substrate functionality was assessed, demonstrating acceptable enhancement (≥100×). The chemisorption of thiols during the first step and the formation of SLB from the vesicles during the second step, were independently monitored by using a quartz crystal microbalance with dissipation monitoring (QCM-D) technique. The SLB membranes represent a simplified model system of the living cells membranes, which makes the successful observation of SERS on these films promising in view of the use of tAPA–Au substrates as a platform for the development of surface-enhanced Raman spectroscopy (SERS) biosensors on living cells. In the future, these tAPA–Au-SLB substrates will be investigated also for drug delivery of bioactive agents from the APA pores. |
topic |
anodic porous alumina SERS nanopores supported lipid bilayers thiols |
url |
https://doi.org/10.3762/bjnano.8.8 |
work_keys_str_mv |
AT marcosalerno surfaceenhancedramanscatteringofselfassembledthiolmonolayersandsupportedlipidmembranesonthinanodicporousalumina AT amirrezashayganpour surfaceenhancedramanscatteringofselfassembledthiolmonolayersandsupportedlipidmembranesonthinanodicporousalumina AT barbarasalis surfaceenhancedramanscatteringofselfassembledthiolmonolayersandsupportedlipidmembranesonthinanodicporousalumina AT silviadante surfaceenhancedramanscatteringofselfassembledthiolmonolayersandsupportedlipidmembranesonthinanodicporousalumina |
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1725110087988543488 |