AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication
Summary: Impairments in synapse development are thought to cause numerous psychiatric disorders. Autism susceptibility candidate 2 (AUTS2) gene has been associated with various psychiatric disorders, such as autism and intellectual disabilities. Although roles for AUTS2 in neuronal migration and neu...
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Elsevier
2020-06-01
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Series: | iScience |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220303680 |
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doaj-549c93a6eb7d439aace1a24299f58f6e |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kei Hori Kunihiko Yamashiro Taku Nagai Wei Shan Saki F. Egusa Kazumi Shimaoka Hiroshi Kuniishi Masayuki Sekiguchi Yasuhiro Go Shoji Tatsumoto Mitsuyo Yamada Reika Shiraishi Kouta Kanno Satoshi Miyashita Asami Sakamoto Manabu Abe Kenji Sakimura Masaki Sone Kazuhiro Sohya Hiroshi Kunugi Keiji Wada Mitsuhiko Yamada Kiyofumi Yamada Mikio Hoshino |
spellingShingle |
Kei Hori Kunihiko Yamashiro Taku Nagai Wei Shan Saki F. Egusa Kazumi Shimaoka Hiroshi Kuniishi Masayuki Sekiguchi Yasuhiro Go Shoji Tatsumoto Mitsuyo Yamada Reika Shiraishi Kouta Kanno Satoshi Miyashita Asami Sakamoto Manabu Abe Kenji Sakimura Masaki Sone Kazuhiro Sohya Hiroshi Kunugi Keiji Wada Mitsuhiko Yamada Kiyofumi Yamada Mikio Hoshino AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication iScience Neuroscience Behavioral Neuroscience Molecular Neuroscience Transcriptomics |
author_facet |
Kei Hori Kunihiko Yamashiro Taku Nagai Wei Shan Saki F. Egusa Kazumi Shimaoka Hiroshi Kuniishi Masayuki Sekiguchi Yasuhiro Go Shoji Tatsumoto Mitsuyo Yamada Reika Shiraishi Kouta Kanno Satoshi Miyashita Asami Sakamoto Manabu Abe Kenji Sakimura Masaki Sone Kazuhiro Sohya Hiroshi Kunugi Keiji Wada Mitsuhiko Yamada Kiyofumi Yamada Mikio Hoshino |
author_sort |
Kei Hori |
title |
AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication |
title_short |
AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication |
title_full |
AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication |
title_fullStr |
AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication |
title_full_unstemmed |
AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social Communication |
title_sort |
auts2 regulation of synapses for proper synaptic inputs and social communication |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2020-06-01 |
description |
Summary: Impairments in synapse development are thought to cause numerous psychiatric disorders. Autism susceptibility candidate 2 (AUTS2) gene has been associated with various psychiatric disorders, such as autism and intellectual disabilities. Although roles for AUTS2 in neuronal migration and neuritogenesis have been reported, its involvement in synapse regulation remains unclear. In this study, we found that excitatory synapses were specifically increased in the Auts2-deficient primary cultured neurons as well as Auts2 mutant forebrains. Electrophysiological recordings and immunostaining showed increases in excitatory synaptic inputs as well as c-fos expression in Auts2 mutant brains, suggesting that an altered balance of excitatory and inhibitory inputs enhances brain excitability. Auts2 mutant mice exhibited autistic-like behaviors including impairments in social interaction and altered vocal communication. Together, these findings suggest that AUTS2 regulates excitatory synapse number to coordinate E/I balance in the brain, whose impairment may underlie the pathology of psychiatric disorders in individuals with AUTS2 mutations. |
topic |
Neuroscience Behavioral Neuroscience Molecular Neuroscience Transcriptomics |
url |
http://www.sciencedirect.com/science/article/pii/S2589004220303680 |
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1724765622108160000 |
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doaj-549c93a6eb7d439aace1a24299f58f6e2020-11-25T02:44:51ZengElsevieriScience2589-00422020-06-01236101183AUTS2 Regulation of Synapses for Proper Synaptic Inputs and Social CommunicationKei Hori0Kunihiko Yamashiro1Taku Nagai2Wei Shan3Saki F. Egusa4Kazumi Shimaoka5Hiroshi Kuniishi6Masayuki Sekiguchi7Yasuhiro Go8Shoji Tatsumoto9Mitsuyo Yamada10Reika Shiraishi11Kouta Kanno12Satoshi Miyashita13Asami Sakamoto14Manabu Abe15Kenji Sakimura16Masaki Sone17Kazuhiro Sohya18Hiroshi Kunugi19Keiji Wada20Mitsuhiko Yamada21Kiyofumi Yamada22Mikio Hoshino23Department of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, Japan; Corresponding authorDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanDepartment of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Neuropsychopharmacology, National Institute of Mental Health, NCNP, Tokyo, 187-8502, Japan; Department of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Tokyo, 187-8502, JapanDepartment of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Tokyo, 187-8502, JapanCognitive Genomics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, Japan; School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8585, Japan; Department of Physiological Sciences, National Institute for Physiological Sciences, Okazaki, Aichi 444-8585, JapanCognitive Genomics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, Japan; Department of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Humanities, Faculty of Law, Economics and the Humanities, Kagoshima University, Kagoshima 890-0065, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Cellular Neurobiology, Brain Research Institute, Niigata University, Nigata 951-8585, JapanDepartment of Cellular Neurobiology, Brain Research Institute, Niigata University, Nigata 951-8585, JapanDepartment of Biomolecular Science, Faculty of Science, Toho University, Chiba 274-8510, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Mental Disorder Research, National Institute of Neuroscience, NCNP, Tokyo 187-8502, JapanDepartment of Degenerative Neurological Diseases, National Institute of Neuroscience, NCNP, Tokyo, 187-8502, JapanDepartment of Neuropsychopharmacology, National Institute of Mental Health, NCNP, Tokyo, 187-8502, JapanDepartment of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine, Nagoya 466-8560, JapanDepartment of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, Tokyo 187-8502, Japan; Corresponding authorSummary: Impairments in synapse development are thought to cause numerous psychiatric disorders. Autism susceptibility candidate 2 (AUTS2) gene has been associated with various psychiatric disorders, such as autism and intellectual disabilities. Although roles for AUTS2 in neuronal migration and neuritogenesis have been reported, its involvement in synapse regulation remains unclear. In this study, we found that excitatory synapses were specifically increased in the Auts2-deficient primary cultured neurons as well as Auts2 mutant forebrains. Electrophysiological recordings and immunostaining showed increases in excitatory synaptic inputs as well as c-fos expression in Auts2 mutant brains, suggesting that an altered balance of excitatory and inhibitory inputs enhances brain excitability. Auts2 mutant mice exhibited autistic-like behaviors including impairments in social interaction and altered vocal communication. Together, these findings suggest that AUTS2 regulates excitatory synapse number to coordinate E/I balance in the brain, whose impairment may underlie the pathology of psychiatric disorders in individuals with AUTS2 mutations.http://www.sciencedirect.com/science/article/pii/S2589004220303680NeuroscienceBehavioral NeuroscienceMolecular NeuroscienceTranscriptomics |