Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung
Mitochondrial biogenesis and adequate energy production in various organs of mammals are necessary for postnatal adaptation to extrauterine life in an environment with high oxygen content. Even though transgenic mice are frequently used as experimental models, to date, no combined detailed molecular...
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doaj-54a2ae78d033489ba339aeb365413fe62020-11-24T23:24:04ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942017-01-01201710.1155/2017/91691469169146Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse LungNatalia El-Merhie0Eveline Baumgart-Vogt1Adrian Pilatz2Susanne Pfreimer3Bianca Pfeiffer4Oleg Pak5Djuro Kosanovic6Michael Seimetz7Ralph Theo Schermuly8Norbert Weissmann9Srikanth Karnati10Institute for Anatomy and Cell Biology II, Division of Medical Cell Biology, Justus Liebig University, Giessen, GermanyInstitute for Anatomy and Cell Biology II, Division of Medical Cell Biology, Justus Liebig University, Giessen, GermanyDepartment of Urology, Pediatric Urology and Andrology, Justus Liebig University, Giessen, GermanyInstitute for Anatomy and Cell Biology II, Division of Medical Cell Biology, Justus Liebig University, Giessen, GermanyInstitute for Anatomy and Cell Biology II, Division of Medical Cell Biology, Justus Liebig University, Giessen, GermanyExcellence Cluster Cardio-Pulmonary System (ECCPS), German Lung Center (DZL), Universities of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University, Giessen, GermanyExcellence Cluster Cardio-Pulmonary System (ECCPS), German Lung Center (DZL), Universities of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University, Giessen, GermanyExcellence Cluster Cardio-Pulmonary System (ECCPS), German Lung Center (DZL), Universities of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University, Giessen, GermanyExcellence Cluster Cardio-Pulmonary System (ECCPS), German Lung Center (DZL), Universities of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University, Giessen, GermanyExcellence Cluster Cardio-Pulmonary System (ECCPS), German Lung Center (DZL), Universities of Giessen and Marburg Lung Center (UGMLC), Justus Liebig University, Giessen, GermanyInstitute for Anatomy and Cell Biology II, Division of Medical Cell Biology, Justus Liebig University, Giessen, GermanyMitochondrial biogenesis and adequate energy production in various organs of mammals are necessary for postnatal adaptation to extrauterine life in an environment with high oxygen content. Even though transgenic mice are frequently used as experimental models, to date, no combined detailed molecular and morphological analysis on the mitochondrial compartment in different lung cell types has been performed during postnatal mouse lung development. In our study, we revealed a significant upregulation of most mitochondrial respiratory complexes at protein and mRNA levels in the lungs of P15 and adult animals in comparison to newborns. The majority of adult animal samples showed the strongest increase, except for succinate dehydrogenase protein (SDHD). Likewise, an increase in mRNA expression for mtDNA transcription machinery genes (Polrmt, Tfam, Tfb1m, and Tfb2m), mitochondrially encoded RNA (mt-Rnr1 and mt-Rnr2), and the nuclear-encoded mitochondrial DNA polymerase (POLG) was observed. The biochemical and molecular results were corroborated by a parallel increase of mitochondrial number, size, cristae number, and complexity, exhibiting heterogeneous patterns in distinct bronchiolar and alveolar epithelial cells. Taken together, our results suggest a specific adaptation and differential maturation of the mitochondrial compartment according to the metabolic needs of individual cell types during postnatal development of the mouse lung.http://dx.doi.org/10.1155/2017/9169146 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia El-Merhie Eveline Baumgart-Vogt Adrian Pilatz Susanne Pfreimer Bianca Pfeiffer Oleg Pak Djuro Kosanovic Michael Seimetz Ralph Theo Schermuly Norbert Weissmann Srikanth Karnati |
spellingShingle |
Natalia El-Merhie Eveline Baumgart-Vogt Adrian Pilatz Susanne Pfreimer Bianca Pfeiffer Oleg Pak Djuro Kosanovic Michael Seimetz Ralph Theo Schermuly Norbert Weissmann Srikanth Karnati Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung Oxidative Medicine and Cellular Longevity |
author_facet |
Natalia El-Merhie Eveline Baumgart-Vogt Adrian Pilatz Susanne Pfreimer Bianca Pfeiffer Oleg Pak Djuro Kosanovic Michael Seimetz Ralph Theo Schermuly Norbert Weissmann Srikanth Karnati |
author_sort |
Natalia El-Merhie |
title |
Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung |
title_short |
Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung |
title_full |
Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung |
title_fullStr |
Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung |
title_full_unstemmed |
Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung |
title_sort |
differential alterations of the mitochondrial morphology and respiratory chain complexes during postnatal development of the mouse lung |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2017-01-01 |
description |
Mitochondrial biogenesis and adequate energy production in various organs of mammals are necessary for postnatal adaptation to extrauterine life in an environment with high oxygen content. Even though transgenic mice are frequently used as experimental models, to date, no combined detailed molecular and morphological analysis on the mitochondrial compartment in different lung cell types has been performed during postnatal mouse lung development. In our study, we revealed a significant upregulation of most mitochondrial respiratory complexes at protein and mRNA levels in the lungs of P15 and adult animals in comparison to newborns. The majority of adult animal samples showed the strongest increase, except for succinate dehydrogenase protein (SDHD). Likewise, an increase in mRNA expression for mtDNA transcription machinery genes (Polrmt, Tfam, Tfb1m, and Tfb2m), mitochondrially encoded RNA (mt-Rnr1 and mt-Rnr2), and the nuclear-encoded mitochondrial DNA polymerase (POLG) was observed. The biochemical and molecular results were corroborated by a parallel increase of mitochondrial number, size, cristae number, and complexity, exhibiting heterogeneous patterns in distinct bronchiolar and alveolar epithelial cells. Taken together, our results suggest a specific adaptation and differential maturation of the mitochondrial compartment according to the metabolic needs of individual cell types during postnatal development of the mouse lung. |
url |
http://dx.doi.org/10.1155/2017/9169146 |
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