Metabolomic response of Calotropis procera growing in the desert to changes in water availability.
Water availability is a major limitation for agricultural productivity. Plants growing in severe arid climates such as deserts provide tools for studying plant growth and performance under extreme drought conditions. The perennial species Calotropis procera used in this study is a shrub growing in m...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3919747?pdf=render |
id |
doaj-54a9ea77d7eb4dc1b3b68e7f05d87ce1 |
---|---|
record_format |
Article |
spelling |
doaj-54a9ea77d7eb4dc1b3b68e7f05d87ce12020-11-24T21:45:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8789510.1371/journal.pone.0087895Metabolomic response of Calotropis procera growing in the desert to changes in water availability.Ahmed RamadanJamal S M SabirSaleha Y M AlakilliAhmed M ShokryNour O GadallaSherif EdrisMagdy A Al-KordyHassan S Al-ZahraniFotouh M El-DomyatiAhmed BahieldinNeil R BakerLothar WillmitzerSusann IrgangWater availability is a major limitation for agricultural productivity. Plants growing in severe arid climates such as deserts provide tools for studying plant growth and performance under extreme drought conditions. The perennial species Calotropis procera used in this study is a shrub growing in many arid areas which has an exceptional ability to adapt and be productive in severe arid conditions. We describe the results of studying the metabolomic response of wild C procera plants growing in the desert to a one time water supply. Leaves of C. procera plants were taken at three time points before and 1 hour, 6 hours and 12 hours after watering and subjected to a metabolomics and lipidomics analysis. Analysis of the data reveals that within one hour after watering C. procera has already responded on the metabolic level to the sudden water availability as evidenced by major changes such as increased levels of most amino acids, a decrease in sucrose, raffinose and maltitol, a decrease in storage lipids (triacylglycerols) and an increase in membrane lipids including photosynthetic membranes. These changes still prevail at the 6 hour time point after watering however 12 hours after watering the metabolomics data are essentially indistinguishable from the prewatering state thus demonstrating not only a rapid response to water availability but also a rapid response to loss of water. Taken together these data suggest that the ability of C. procera to survive under the very harsh drought conditions prevailing in the desert might be associated with its rapid adjustments to water availability and losses.http://europepmc.org/articles/PMC3919747?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Ramadan Jamal S M Sabir Saleha Y M Alakilli Ahmed M Shokry Nour O Gadalla Sherif Edris Magdy A Al-Kordy Hassan S Al-Zahrani Fotouh M El-Domyati Ahmed Bahieldin Neil R Baker Lothar Willmitzer Susann Irgang |
spellingShingle |
Ahmed Ramadan Jamal S M Sabir Saleha Y M Alakilli Ahmed M Shokry Nour O Gadalla Sherif Edris Magdy A Al-Kordy Hassan S Al-Zahrani Fotouh M El-Domyati Ahmed Bahieldin Neil R Baker Lothar Willmitzer Susann Irgang Metabolomic response of Calotropis procera growing in the desert to changes in water availability. PLoS ONE |
author_facet |
Ahmed Ramadan Jamal S M Sabir Saleha Y M Alakilli Ahmed M Shokry Nour O Gadalla Sherif Edris Magdy A Al-Kordy Hassan S Al-Zahrani Fotouh M El-Domyati Ahmed Bahieldin Neil R Baker Lothar Willmitzer Susann Irgang |
author_sort |
Ahmed Ramadan |
title |
Metabolomic response of Calotropis procera growing in the desert to changes in water availability. |
title_short |
Metabolomic response of Calotropis procera growing in the desert to changes in water availability. |
title_full |
Metabolomic response of Calotropis procera growing in the desert to changes in water availability. |
title_fullStr |
Metabolomic response of Calotropis procera growing in the desert to changes in water availability. |
title_full_unstemmed |
Metabolomic response of Calotropis procera growing in the desert to changes in water availability. |
title_sort |
metabolomic response of calotropis procera growing in the desert to changes in water availability. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Water availability is a major limitation for agricultural productivity. Plants growing in severe arid climates such as deserts provide tools for studying plant growth and performance under extreme drought conditions. The perennial species Calotropis procera used in this study is a shrub growing in many arid areas which has an exceptional ability to adapt and be productive in severe arid conditions. We describe the results of studying the metabolomic response of wild C procera plants growing in the desert to a one time water supply. Leaves of C. procera plants were taken at three time points before and 1 hour, 6 hours and 12 hours after watering and subjected to a metabolomics and lipidomics analysis. Analysis of the data reveals that within one hour after watering C. procera has already responded on the metabolic level to the sudden water availability as evidenced by major changes such as increased levels of most amino acids, a decrease in sucrose, raffinose and maltitol, a decrease in storage lipids (triacylglycerols) and an increase in membrane lipids including photosynthetic membranes. These changes still prevail at the 6 hour time point after watering however 12 hours after watering the metabolomics data are essentially indistinguishable from the prewatering state thus demonstrating not only a rapid response to water availability but also a rapid response to loss of water. Taken together these data suggest that the ability of C. procera to survive under the very harsh drought conditions prevailing in the desert might be associated with its rapid adjustments to water availability and losses. |
url |
http://europepmc.org/articles/PMC3919747?pdf=render |
work_keys_str_mv |
AT ahmedramadan metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT jamalsmsabir metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT salehaymalakilli metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT ahmedmshokry metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT nourogadalla metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT sherifedris metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT magdyaalkordy metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT hassansalzahrani metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT fotouhmeldomyati metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT ahmedbahieldin metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT neilrbaker metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT lotharwillmitzer metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability AT susannirgang metabolomicresponseofcalotropisproceragrowinginthedeserttochangesinwateravailability |
_version_ |
1725906381320486912 |