Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc
Twenty weaned piglets with initial body weight of 6.83 ± 0.33 kg (21 day of age, LYD) were randomly assigned to four treatments for a two-week feeding trial to determine the effects of different dietary zinc on nutrient digestibility, intestinal health, and microbiome of weaned piglets. The dietary...
Main Authors: | , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-05-01
|
Series: | Animals |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2615/11/5/1356 |
id |
doaj-5504519a27b244bf9d049535ebb5f994 |
---|---|
record_format |
Article |
spelling |
doaj-5504519a27b244bf9d049535ebb5f9942021-05-31T23:39:02ZengMDPI AGAnimals2076-26152021-05-01111356135610.3390/ani11051356Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of ZincHan-Jin Oh0Yei-Ju Park1Jae Hyoung Cho2Min-Ho Song3Bon-Hee Gu4Won Yun5Ji-Hwan Lee6Ji-Seon An7Yong-Ju Kim8Jun-Soeng Lee9Sheena Kim10Hyeri Kim11Eun Sol Kim12Byoung-Kon Lee13Byeong-Woo Kim14Hyeun Bum Kim15Jin-Ho Cho16Myung-Hoo Kim17Department of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Pusan National University, Miryang 50463, KoreaDepartment of Animal Resource, and Science, Dankook University, Cheonan 311-16, KoreaDivision of Animal and Dairy Science, Chungnam National University, Daejeon 341-34, KoreaLife and Industry Convergence Research Institute, Pusan National University, Mirayng 50463, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Resource, and Science, Dankook University, Cheonan 311-16, KoreaDepartment of Animal Resource, and Science, Dankook University, Cheonan 311-16, KoreaDepartment of Animal Resource, and Science, Dankook University, Cheonan 311-16, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Pusan National University, Miryang 50463, KoreaDepartment of Animal Resource, and Science, Dankook University, Cheonan 311-16, KoreaDepartment of Animal Sciences, Chungbuk National University, Cheongju 286-44, KoreaDepartment of Animal Sciences, Pusan National University, Miryang 50463, KoreaTwenty weaned piglets with initial body weight of 6.83 ± 0.33 kg (21 day of age, LYD) were randomly assigned to four treatments for a two-week feeding trial to determine the effects of different dietary zinc on nutrient digestibility, intestinal health, and microbiome of weaned piglets. The dietary treatments included a negative control (CON), standard ZnO (ZnO, 2500 ppm), zinc chelate with glycine (Chelate-ZnO, 200 ppm), and nanoparticle-sized ZnO (Nano-ZnO, 200 ppm). At 0 to 1 week, the diarrhea score was decreased in the CON group compared with the ZnO, Chelate-ZnO, and Nano-ZnO group. In overall period, the ZnO and Nano-ZnO groups exhibited improved diarrhea scores compared to the CON group. The apparent total tract digestibility of dry matter and gross energy was the lowest in the CON group after one week. Compared to the ZnO group, the chelate-ZnO group exhibited higher proportion of T-bet+ and FoxP3+ T cells and the nano-ZnO group had higher numbers of RORgt+ and GATA3+ T cells in the mesenteric lymph nodes. ZnO group increased IL-6 and IL-8 levels in the colon tissues and these positive effects were observed in both chelate ZnO and nano-ZnO groups with lower level. The 16S rRNA gene analysis showed that the relative abundance of <i>Prevotella</i> was higher in the ZnO-treated groups than in the CON group and that of <i>Succinivibrio</i> was the highest in the nano-ZnO group. The relative abundance of <i>Lactobacillus</i> increased in the ZnO group. In conclusion, low nano-ZnO levels have similar effects on nutrient digestibility, fecal microflora, and intestinal immune profiles in weaning pigs; thus, nano-ZnO could be used as a ZnO alternative for promoting ZnO utilization and intestinal immunity.https://www.mdpi.com/2076-2615/11/5/1356ZnO alternativepigletsdigestibilityimmunemicrobiome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han-Jin Oh Yei-Ju Park Jae Hyoung Cho Min-Ho Song Bon-Hee Gu Won Yun Ji-Hwan Lee Ji-Seon An Yong-Ju Kim Jun-Soeng Lee Sheena Kim Hyeri Kim Eun Sol Kim Byoung-Kon Lee Byeong-Woo Kim Hyeun Bum Kim Jin-Ho Cho Myung-Hoo Kim |
spellingShingle |
Han-Jin Oh Yei-Ju Park Jae Hyoung Cho Min-Ho Song Bon-Hee Gu Won Yun Ji-Hwan Lee Ji-Seon An Yong-Ju Kim Jun-Soeng Lee Sheena Kim Hyeri Kim Eun Sol Kim Byoung-Kon Lee Byeong-Woo Kim Hyeun Bum Kim Jin-Ho Cho Myung-Hoo Kim Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc Animals ZnO alternative piglets digestibility immune microbiome |
author_facet |
Han-Jin Oh Yei-Ju Park Jae Hyoung Cho Min-Ho Song Bon-Hee Gu Won Yun Ji-Hwan Lee Ji-Seon An Yong-Ju Kim Jun-Soeng Lee Sheena Kim Hyeri Kim Eun Sol Kim Byoung-Kon Lee Byeong-Woo Kim Hyeun Bum Kim Jin-Ho Cho Myung-Hoo Kim |
author_sort |
Han-Jin Oh |
title |
Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc |
title_short |
Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc |
title_full |
Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc |
title_fullStr |
Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc |
title_full_unstemmed |
Changes in Diarrhea Score, Nutrient Digestibility, Zinc Utilization, Intestinal Immune Profiles, and Fecal Microbiome in Weaned Piglets by Different Forms of Zinc |
title_sort |
changes in diarrhea score, nutrient digestibility, zinc utilization, intestinal immune profiles, and fecal microbiome in weaned piglets by different forms of zinc |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2021-05-01 |
description |
Twenty weaned piglets with initial body weight of 6.83 ± 0.33 kg (21 day of age, LYD) were randomly assigned to four treatments for a two-week feeding trial to determine the effects of different dietary zinc on nutrient digestibility, intestinal health, and microbiome of weaned piglets. The dietary treatments included a negative control (CON), standard ZnO (ZnO, 2500 ppm), zinc chelate with glycine (Chelate-ZnO, 200 ppm), and nanoparticle-sized ZnO (Nano-ZnO, 200 ppm). At 0 to 1 week, the diarrhea score was decreased in the CON group compared with the ZnO, Chelate-ZnO, and Nano-ZnO group. In overall period, the ZnO and Nano-ZnO groups exhibited improved diarrhea scores compared to the CON group. The apparent total tract digestibility of dry matter and gross energy was the lowest in the CON group after one week. Compared to the ZnO group, the chelate-ZnO group exhibited higher proportion of T-bet+ and FoxP3+ T cells and the nano-ZnO group had higher numbers of RORgt+ and GATA3+ T cells in the mesenteric lymph nodes. ZnO group increased IL-6 and IL-8 levels in the colon tissues and these positive effects were observed in both chelate ZnO and nano-ZnO groups with lower level. The 16S rRNA gene analysis showed that the relative abundance of <i>Prevotella</i> was higher in the ZnO-treated groups than in the CON group and that of <i>Succinivibrio</i> was the highest in the nano-ZnO group. The relative abundance of <i>Lactobacillus</i> increased in the ZnO group. In conclusion, low nano-ZnO levels have similar effects on nutrient digestibility, fecal microflora, and intestinal immune profiles in weaning pigs; thus, nano-ZnO could be used as a ZnO alternative for promoting ZnO utilization and intestinal immunity. |
topic |
ZnO alternative piglets digestibility immune microbiome |
url |
https://www.mdpi.com/2076-2615/11/5/1356 |
work_keys_str_mv |
AT hanjinoh changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT yeijupark changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT jaehyoungcho changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT minhosong changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT bonheegu changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT wonyun changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT jihwanlee changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT jiseonan changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT yongjukim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT junsoenglee changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT sheenakim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT hyerikim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT eunsolkim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT byoungkonlee changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT byeongwookim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT hyeunbumkim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT jinhocho changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc AT myunghookim changesindiarrheascorenutrientdigestibilityzincutilizationintestinalimmuneprofilesandfecalmicrobiomeinweanedpigletsbydifferentformsofzinc |
_version_ |
1721416928843530240 |