Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches
Effect of lignocellulosic medium supplemented with selected easily-metabolised carbon sources on microbial xylanase production was assessed. A newly isolated oil-palm-waste-domesticated bio-agent-producing fungus, identified based on rRNA analysis as T. asperellum USM SD4 was used as a representativ...
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North Carolina State University
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doaj-5507eb2c398e4150aad93bfe7f0197b22020-11-24T21:37:16ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-09-011149611962710.15376/biores.11.4.9611-9627Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit BunchesKamoldeen Abiodun Ajijolakewu0Cheu Peng Leh1Wan Nadiah Wan AbduLLah2Chee Keong Lee3Universiti Sains Malaysia; MalaysiaSchool of Industrial Technology, Universiti Sains Malaysia, 1800USM, Penang Malaysia; MalaysiaSchool of Industrial Technology, Universiti Sains Malaysia, 1800USM, Penang Malaysia; MalaysiaSchool of Industrial Technology, Universiti Sains Malaysia, 1800USM, Penang Malaysia; MalaysiaEffect of lignocellulosic medium supplemented with selected easily-metabolised carbon sources on microbial xylanase production was assessed. A newly isolated oil-palm-waste-domesticated bio-agent-producing fungus, identified based on rRNA analysis as T. asperellum USM SD4 was used as a representative organism. The potential of T. asperellum for enhanced xylanase production was evaluated by the statistical optimization of important cultural parameters via response surface methodology (RSM). T. asperellum showed optimum xylanase activity at pH 7; temperature 27 °C; moisture content 4 mL growth medium (gm): 1 gram dried substrate (gds) and inoculum size 2 x 106 spores/mL. Xylanase activity (2,337 IU/gds) attained in this study was far higher than ever reported for T. asperellum. Using the set of optimum conditions, the mixture of supplementary sugars to the lignocellulosic medium initiated xylanase repression in a concentration-independent manner. However, the degree of repression depended on the nature and type of respectively added sugar. The repressive effect exerted by monosaccharides (xylose, glucose, and fructose) was greater than exerted by either of dimeric (cellobiose and sucrose) or polymeric (xylan) sugars. Of all added substrates, xylan exerted the least repressive effect. Using xylose as a representative sugar, mechanism of xylanase repression was decisively explained and supported with experimental data.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_9611_Ajijolakewu_Easily_Metabolised_Carbon_XylanaseBiomass utilizationXylanaseOPEFBXylanase repressionEffect of reducing sugarsTrichoderma asperellum USM SD4Response surface methodology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kamoldeen Abiodun Ajijolakewu Cheu Peng Leh Wan Nadiah Wan AbduLLah Chee Keong Lee |
spellingShingle |
Kamoldeen Abiodun Ajijolakewu Cheu Peng Leh Wan Nadiah Wan AbduLLah Chee Keong Lee Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches BioResources Biomass utilization Xylanase OPEFB Xylanase repression Effect of reducing sugars Trichoderma asperellum USM SD4 Response surface methodology |
author_facet |
Kamoldeen Abiodun Ajijolakewu Cheu Peng Leh Wan Nadiah Wan AbduLLah Chee Keong Lee |
author_sort |
Kamoldeen Abiodun Ajijolakewu |
title |
Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches |
title_short |
Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches |
title_full |
Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches |
title_fullStr |
Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches |
title_full_unstemmed |
Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches |
title_sort |
assessment of the effect of easily-metabolised carbon supplements on xylanase production by newly isolated trichoderma asperellum usm sd4 cultivated on oil palm empty fruit bunches |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 1930-2126 |
publishDate |
2016-09-01 |
description |
Effect of lignocellulosic medium supplemented with selected easily-metabolised carbon sources on microbial xylanase production was assessed. A newly isolated oil-palm-waste-domesticated bio-agent-producing fungus, identified based on rRNA analysis as T. asperellum USM SD4 was used as a representative organism. The potential of T. asperellum for enhanced xylanase production was evaluated by the statistical optimization of important cultural parameters via response surface methodology (RSM). T. asperellum showed optimum xylanase activity at pH 7; temperature 27 °C; moisture content 4 mL growth medium (gm): 1 gram dried substrate (gds) and inoculum size 2 x 106 spores/mL. Xylanase activity (2,337 IU/gds) attained in this study was far higher than ever reported for T. asperellum. Using the set of optimum conditions, the mixture of supplementary sugars to the lignocellulosic medium initiated xylanase repression in a concentration-independent manner. However, the degree of repression depended on the nature and type of respectively added sugar. The repressive effect exerted by monosaccharides (xylose, glucose, and fructose) was greater than exerted by either of dimeric (cellobiose and sucrose) or polymeric (xylan) sugars. Of all added substrates, xylan exerted the least repressive effect. Using xylose as a representative sugar, mechanism of xylanase repression was decisively explained and supported with experimental data. |
topic |
Biomass utilization Xylanase OPEFB Xylanase repression Effect of reducing sugars Trichoderma asperellum USM SD4 Response surface methodology |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_9611_Ajijolakewu_Easily_Metabolised_Carbon_Xylanase |
work_keys_str_mv |
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