Compositional zero-inflated network estimation for microbiome data
Abstract Background The estimation of microbial networks can provide important insight into the ecological relationships among the organisms that comprise the microbiome. However, there are a number of critical statistical challenges in the inference of such networks from high-throughput data. Since...
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doaj-5507f1a05b324445a40d9d62484be09d2021-01-03T12:21:21ZengBMCBMC Bioinformatics1471-21052020-12-0121S2112010.1186/s12859-020-03911-wCompositional zero-inflated network estimation for microbiome dataMin Jin Ha0Junghi Kim1Jessica Galloway-Peña2Kim-Anh Do3Christine B. Peterson4Department of Biostatistics, University of Texas MD Anderson Cancer CenterCenter for Devices and Radiological Health, U.S. Food and Drug AdministrationDepartment of Veterinary Pathobiology, Texas A&M UniversityDepartment of Biostatistics, University of Texas MD Anderson Cancer CenterDepartment of Biostatistics, University of Texas MD Anderson Cancer CenterAbstract Background The estimation of microbial networks can provide important insight into the ecological relationships among the organisms that comprise the microbiome. However, there are a number of critical statistical challenges in the inference of such networks from high-throughput data. Since the abundances in each sample are constrained to have a fixed sum and there is incomplete overlap in microbial populations across subjects, the data are both compositional and zero-inflated. Results We propose the COmpositional Zero-Inflated Network Estimation (COZINE) method for inference of microbial networks which addresses these critical aspects of the data while maintaining computational scalability. COZINE relies on the multivariate Hurdle model to infer a sparse set of conditional dependencies which reflect not only relationships among the continuous values, but also among binary indicators of presence or absence and between the binary and continuous representations of the data. Our simulation results show that the proposed method is better able to capture various types of microbial relationships than existing approaches. We demonstrate the utility of the method with an application to understanding the oral microbiome network in a cohort of leukemic patients. Conclusions Our proposed method addresses important challenges in microbiome network estimation, and can be effectively applied to discover various types of dependence relationships in microbial communities. The procedure we have developed, which we refer to as COZINE, is available online at https://github.com/MinJinHa/COZINE .https://doi.org/10.1186/s12859-020-03911-wMicrobiomeNetworkGraphical modelZero-inflationCompositional data |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Jin Ha Junghi Kim Jessica Galloway-Peña Kim-Anh Do Christine B. Peterson |
spellingShingle |
Min Jin Ha Junghi Kim Jessica Galloway-Peña Kim-Anh Do Christine B. Peterson Compositional zero-inflated network estimation for microbiome data BMC Bioinformatics Microbiome Network Graphical model Zero-inflation Compositional data |
author_facet |
Min Jin Ha Junghi Kim Jessica Galloway-Peña Kim-Anh Do Christine B. Peterson |
author_sort |
Min Jin Ha |
title |
Compositional zero-inflated network estimation for microbiome data |
title_short |
Compositional zero-inflated network estimation for microbiome data |
title_full |
Compositional zero-inflated network estimation for microbiome data |
title_fullStr |
Compositional zero-inflated network estimation for microbiome data |
title_full_unstemmed |
Compositional zero-inflated network estimation for microbiome data |
title_sort |
compositional zero-inflated network estimation for microbiome data |
publisher |
BMC |
series |
BMC Bioinformatics |
issn |
1471-2105 |
publishDate |
2020-12-01 |
description |
Abstract Background The estimation of microbial networks can provide important insight into the ecological relationships among the organisms that comprise the microbiome. However, there are a number of critical statistical challenges in the inference of such networks from high-throughput data. Since the abundances in each sample are constrained to have a fixed sum and there is incomplete overlap in microbial populations across subjects, the data are both compositional and zero-inflated. Results We propose the COmpositional Zero-Inflated Network Estimation (COZINE) method for inference of microbial networks which addresses these critical aspects of the data while maintaining computational scalability. COZINE relies on the multivariate Hurdle model to infer a sparse set of conditional dependencies which reflect not only relationships among the continuous values, but also among binary indicators of presence or absence and between the binary and continuous representations of the data. Our simulation results show that the proposed method is better able to capture various types of microbial relationships than existing approaches. We demonstrate the utility of the method with an application to understanding the oral microbiome network in a cohort of leukemic patients. Conclusions Our proposed method addresses important challenges in microbiome network estimation, and can be effectively applied to discover various types of dependence relationships in microbial communities. The procedure we have developed, which we refer to as COZINE, is available online at https://github.com/MinJinHa/COZINE . |
topic |
Microbiome Network Graphical model Zero-inflation Compositional data |
url |
https://doi.org/10.1186/s12859-020-03911-w |
work_keys_str_mv |
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