Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein
Background: Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospe...
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doaj-556e6655a23b440896852ae5cd5d136d2020-11-25T00:10:23ZengElsevierElectronic Journal of Biotechnology0717-34582019-05-01395260Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle proteinMariana Delgado-García0Adriana C. Flores-Gallegos1Manuel Kirchmayr2Jorge A. Rodríguez3Juan C. Mateos-Díaz4Cristobal N. Aguilar5Marcelo Muller6Rosa M. Camacho-Ruíz7Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, MexicoDepartamento de Investigación en Alimentos, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza, 25280, Saltillo, Coahuila, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, MexicoCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, MexicoDepartamento de Investigación en Alimentos, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza, 25280, Saltillo, Coahuila, MexicoDepartamento de Bioquímica e Biología Molecular, Setor de Ciencias Biológicas, Universidade Federal do Paraná, Curitiba, BrazilCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800, 44270, Guadalajara, Jalisco, Mexico; Corresponding author.Background: Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospection of halophilic microorganisms for the production of proteases to prove their application for peptide production. Results: Halophilic microorganisms were isolated from saline soils of Mexico and Bolivia. An enzymatic screening was carried out for the detection of lipases, esterases, pHB depolymerases, chitinases, and proteases. Most of the strains were able to produce lipases, esterases, and proteases, and larger hydrolysis halos were detected for protease activity. Halobacillus andaensis was selected to be studied for proteolytic activity production; the microorganism was able to grow on gelatin, yeast extract, skim milk, casein, peptone, fish muscle (Cyprinus carpio), and soy flour as protein sources, and among these sources, fish muscle protein was the best inducer of proteolytic activity, achieving a protease production of 571 U/mL. The extracellular protease was active at 50°C, pH 8, and 1.4 M NaCl and was inhibited by phenylmethylsulfonyl fluoride. The proteolytic activity of H. andaensis was used to hydrolyze fish muscle protein for peptide production. The peptides obtained showed a MW of 5.3 kDa and a radical scavenging ability of 10 to 30% on 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and a ferric reducing ability of plasma. Conclusion: The use of noncommercial extracellular protease produced by H. andaensis for biologically active peptide production using fish muscle as the protein source presents a great opportunity for high-value peptide production.How to cite: Delgado-García M, Flores-Gallegos AC, Kirchmayr M, et al. Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein. Electron J Biotechnol 2019;39. https://doi.org/10.1016/j.ejbt.2019.03.001. Keywords: Antioxidant capacity, Bioactive peptide, Bolivia, Enzymes, Esterases, Fish protein, Halobacillus andaensis, Halophilic bacteria, Halophilic microorganisms, Lipases, Mexico, Peptides, Proteasehttp://www.sciencedirect.com/science/article/pii/S0717345819300077 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariana Delgado-García Adriana C. Flores-Gallegos Manuel Kirchmayr Jorge A. Rodríguez Juan C. Mateos-Díaz Cristobal N. Aguilar Marcelo Muller Rosa M. Camacho-Ruíz |
spellingShingle |
Mariana Delgado-García Adriana C. Flores-Gallegos Manuel Kirchmayr Jorge A. Rodríguez Juan C. Mateos-Díaz Cristobal N. Aguilar Marcelo Muller Rosa M. Camacho-Ruíz Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein Electronic Journal of Biotechnology |
author_facet |
Mariana Delgado-García Adriana C. Flores-Gallegos Manuel Kirchmayr Jorge A. Rodríguez Juan C. Mateos-Díaz Cristobal N. Aguilar Marcelo Muller Rosa M. Camacho-Ruíz |
author_sort |
Mariana Delgado-García |
title |
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein |
title_short |
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein |
title_full |
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein |
title_fullStr |
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein |
title_full_unstemmed |
Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein |
title_sort |
bioprospection of proteases from halobacillus andaensis for bioactive peptide production from fish muscle protein |
publisher |
Elsevier |
series |
Electronic Journal of Biotechnology |
issn |
0717-3458 |
publishDate |
2019-05-01 |
description |
Background: Biologically active peptides produced from fish wastes are gaining attention because their health benefits. Proteases produced by halophilic microorganisms are considered as a source of active enzymes in high salt systems like fish residues. Hence, the aim of this study was the bioprospection of halophilic microorganisms for the production of proteases to prove their application for peptide production. Results: Halophilic microorganisms were isolated from saline soils of Mexico and Bolivia. An enzymatic screening was carried out for the detection of lipases, esterases, pHB depolymerases, chitinases, and proteases. Most of the strains were able to produce lipases, esterases, and proteases, and larger hydrolysis halos were detected for protease activity. Halobacillus andaensis was selected to be studied for proteolytic activity production; the microorganism was able to grow on gelatin, yeast extract, skim milk, casein, peptone, fish muscle (Cyprinus carpio), and soy flour as protein sources, and among these sources, fish muscle protein was the best inducer of proteolytic activity, achieving a protease production of 571 U/mL. The extracellular protease was active at 50°C, pH 8, and 1.4 M NaCl and was inhibited by phenylmethylsulfonyl fluoride. The proteolytic activity of H. andaensis was used to hydrolyze fish muscle protein for peptide production. The peptides obtained showed a MW of 5.3 kDa and a radical scavenging ability of 10 to 30% on 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and a ferric reducing ability of plasma. Conclusion: The use of noncommercial extracellular protease produced by H. andaensis for biologically active peptide production using fish muscle as the protein source presents a great opportunity for high-value peptide production.How to cite: Delgado-García M, Flores-Gallegos AC, Kirchmayr M, et al. Bioprospection of proteases from Halobacillus andaensis for bioactive peptide production from fish muscle protein. Electron J Biotechnol 2019;39. https://doi.org/10.1016/j.ejbt.2019.03.001. Keywords: Antioxidant capacity, Bioactive peptide, Bolivia, Enzymes, Esterases, Fish protein, Halobacillus andaensis, Halophilic bacteria, Halophilic microorganisms, Lipases, Mexico, Peptides, Protease |
url |
http://www.sciencedirect.com/science/article/pii/S0717345819300077 |
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