Mycobacterium tuberculosis promotes genomic instability in macrophages
BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instabil...
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Instituto Oswaldo Cruz, Ministério da Saúde
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doaj-557bab4a43884bd8a3c5a789729128cf2020-11-25T02:45:38ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-8060113316116610.1590/0074-02760170281S0074-02762018000300161Mycobacterium tuberculosis promotes genomic instability in macrophagesJorge Castro-GarzaMiriam Lorena Luévano-MartínezLicet Villarreal-TreviñoJaime GosálvezJosé Luis FernándezMartha Imelda Dávila-RodríguezCatalina García-VielmaSilvia González-HernándezElva Irene Cortés-GutiérrezBACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300161&lng=en&tlng=enMycobacterium tuberculosisalkaline-labile sitesDNA breakage detection fluorescence in situ hybridisation (DBD-FISH) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jorge Castro-Garza Miriam Lorena Luévano-Martínez Licet Villarreal-Treviño Jaime Gosálvez José Luis Fernández Martha Imelda Dávila-Rodríguez Catalina García-Vielma Silvia González-Hernández Elva Irene Cortés-Gutiérrez |
spellingShingle |
Jorge Castro-Garza Miriam Lorena Luévano-Martínez Licet Villarreal-Treviño Jaime Gosálvez José Luis Fernández Martha Imelda Dávila-Rodríguez Catalina García-Vielma Silvia González-Hernández Elva Irene Cortés-Gutiérrez Mycobacterium tuberculosis promotes genomic instability in macrophages Memórias do Instituto Oswaldo Cruz. Mycobacterium tuberculosis alkaline-labile sites DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) |
author_facet |
Jorge Castro-Garza Miriam Lorena Luévano-Martínez Licet Villarreal-Treviño Jaime Gosálvez José Luis Fernández Martha Imelda Dávila-Rodríguez Catalina García-Vielma Silvia González-Hernández Elva Irene Cortés-Gutiérrez |
author_sort |
Jorge Castro-Garza |
title |
Mycobacterium tuberculosis promotes genomic instability in macrophages |
title_short |
Mycobacterium tuberculosis promotes genomic instability in macrophages |
title_full |
Mycobacterium tuberculosis promotes genomic instability in macrophages |
title_fullStr |
Mycobacterium tuberculosis promotes genomic instability in macrophages |
title_full_unstemmed |
Mycobacterium tuberculosis promotes genomic instability in macrophages |
title_sort |
mycobacterium tuberculosis promotes genomic instability in macrophages |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
description |
BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages. METHODS We analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe. FINDINGS Quantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells. MAIN CONCLUSIONS Genomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection. |
topic |
Mycobacterium tuberculosis alkaline-labile sites DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000300161&lng=en&tlng=en |
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