In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target
An effective vaccine to reduce malaria transmission is central to control and ultimately achieve disease eradication. Recently, we demonstrated that antibodies targeting the Plasmodium falciparum surface protein P47 (Pfs47) reduce parasite transmission to Anopheles gambiae mosquitoes. Here, Plasmodi...
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doaj-55817fd01c9b4ca0a5e781c4c3f5aff42020-11-25T02:32:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-07-011110.3389/fmicb.2020.01496549514In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine TargetLampouguin Yenkoidiok-Douti0Lampouguin Yenkoidiok-Douti1Gaspar E. Canepa2Ana Beatriz F. Barletta3Carolina Barillas-Mury4Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Rockville, MD, United StatesFischell Department of Bioengineering, University of Maryland, College Park, MD, United StatesLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Rockville, MD, United StatesLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Rockville, MD, United StatesLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Rockville, MD, United StatesAn effective vaccine to reduce malaria transmission is central to control and ultimately achieve disease eradication. Recently, we demonstrated that antibodies targeting the Plasmodium falciparum surface protein P47 (Pfs47) reduce parasite transmission to Anopheles gambiae mosquitoes. Here, Plasmodium berghei (Pb) was used as a model to assess the in vivo efficacy of a P47-targeted transmission blocking vaccine (Pbs47). Mice were immunized following a prime/boost regimen and infected with P. berghei. The effect of immunization on infectivity to mosquitoes was evaluated by direct feeding on P. berghei-infected mice. The key region in Pbs47 where antibody binding confers protection was mapped, and the immunogenicity of this protective antigen was enhanced by conjugation to a virus-like particle. Passive immunization with 100 and 50 μg/mL of anti-Pbs47 IgG reduced oocyst density by 77 and 67%, respectively. Furthermore, affinity purified Pbs47-specific IgG significantly reduced oocyst density by 88 and 77%, respectively at doses as low as 10 and 1 μg/mL. These studies suggest that P47 is a promising transmission blocking target and show that antibodies to the same specific region in Pfs47 and Pbs47 confer protection.https://www.frontiersin.org/article/10.3389/fmicb.2020.01496/fullmalariaP47transmission-blocking vaccinesVLPspassive immunizationAnopheles gambiae |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lampouguin Yenkoidiok-Douti Lampouguin Yenkoidiok-Douti Gaspar E. Canepa Ana Beatriz F. Barletta Carolina Barillas-Mury |
spellingShingle |
Lampouguin Yenkoidiok-Douti Lampouguin Yenkoidiok-Douti Gaspar E. Canepa Ana Beatriz F. Barletta Carolina Barillas-Mury In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target Frontiers in Microbiology malaria P47 transmission-blocking vaccines VLPs passive immunization Anopheles gambiae |
author_facet |
Lampouguin Yenkoidiok-Douti Lampouguin Yenkoidiok-Douti Gaspar E. Canepa Ana Beatriz F. Barletta Carolina Barillas-Mury |
author_sort |
Lampouguin Yenkoidiok-Douti |
title |
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target |
title_short |
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target |
title_full |
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target |
title_fullStr |
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target |
title_full_unstemmed |
In vivo Characterization of Plasmodium berghei P47 (Pbs47) as a Malaria Transmission-Blocking Vaccine Target |
title_sort |
in vivo characterization of plasmodium berghei p47 (pbs47) as a malaria transmission-blocking vaccine target |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-07-01 |
description |
An effective vaccine to reduce malaria transmission is central to control and ultimately achieve disease eradication. Recently, we demonstrated that antibodies targeting the Plasmodium falciparum surface protein P47 (Pfs47) reduce parasite transmission to Anopheles gambiae mosquitoes. Here, Plasmodium berghei (Pb) was used as a model to assess the in vivo efficacy of a P47-targeted transmission blocking vaccine (Pbs47). Mice were immunized following a prime/boost regimen and infected with P. berghei. The effect of immunization on infectivity to mosquitoes was evaluated by direct feeding on P. berghei-infected mice. The key region in Pbs47 where antibody binding confers protection was mapped, and the immunogenicity of this protective antigen was enhanced by conjugation to a virus-like particle. Passive immunization with 100 and 50 μg/mL of anti-Pbs47 IgG reduced oocyst density by 77 and 67%, respectively. Furthermore, affinity purified Pbs47-specific IgG significantly reduced oocyst density by 88 and 77%, respectively at doses as low as 10 and 1 μg/mL. These studies suggest that P47 is a promising transmission blocking target and show that antibodies to the same specific region in Pfs47 and Pbs47 confer protection. |
topic |
malaria P47 transmission-blocking vaccines VLPs passive immunization Anopheles gambiae |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01496/full |
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