Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering
The spleen is considered a non-essential organ. However, its importance is increasingly clear, given the serious disorders caused by its absence or dysfunction, e.g., greater susceptibility to infections, thromboembolism and cancer. Surgical techniques to preserve the spleen and maintain splenic fun...
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2020-10-01
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doaj-558d9051ac8e475a98f8b32c31de53a72020-11-25T03:55:36ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.573461573461Decellularized Splenic Matrix as a Scaffold for Spleen BioengineeringTadeu Ériton Caliman Zanardo0Tadeu Ériton Caliman Zanardo1Fernanda Gobbi Amorim2Fernanda Gobbi Amorim3Gabriel Henrique Taufner4Gabriel Henrique Taufner5Rayssa Helena Arruda Pereira6Rayssa Helena Arruda Pereira7Ian Manhoni Baiense8Afrânio Côgo Destefani9Afrânio Côgo Destefani10Leo Kei Iwai11Raul Cavalcante Maranhão12Breno Valentim Nogueira13Breno Valentim Nogueira14Biotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilBiotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilPharmaceutical Sciences Graduate Program, University of Vila Velha, Vila Velha, BrazilBiotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilBiotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilBiotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilLaboratory of Proteomics and Mass Spectrometry-Special Laboratory of Applied Toxinology LETA/CETICS, Instituto Butantan, São Paulo, BrazilHeart Institute (InCor), Medical School Hospital, University of São Paulo, São Paulo, BrazilBiotechnology Graduate Program, Rede Nordeste de Biotecnologia (RENORBIO), Vitória, BrazilTissue Engineering Core, Department of Morphology, Federal University of Espírito Santo, Vitória, BrazilThe spleen is considered a non-essential organ. However, its importance is increasingly clear, given the serious disorders caused by its absence or dysfunction, e.g., greater susceptibility to infections, thromboembolism and cancer. Surgical techniques to preserve the spleen and maintain splenic function have become increasingly common. However, the morbidity and mortality associated with its absence and dysfunction are still high. We used the decellularization technique to obtain a viable splenic scaffold for recellularization in vitro and propose the idea of bioengineered spleen transplantation to the host. We observed the maintenance of important structural components such as white pulp, marginal zone and red pulp, in addition to the network of vascular ducts. The decellularized scaffold presents minimal residual DNA and SDS, which are essential to prevent immunogenic responses and transplantation failure. Also, the main components of the splenic matrix were preserved after decellularization, with retention of approximately 72% in the matrisomal protein content. The scaffold we developed was partially recellularized with stromal cells from the spleen of neonatal rats, demonstrating adhesion, proliferation and viability of cells. Therefore, the splenic scaffold is very promising for use in studies on spleen reconstruction and transplantation, with the aim of complete recovery of splenic function.https://www.frontiersin.org/article/10.3389/fbioe.2020.573461/fullsplenic scaffolddecellularizationextracellular matrixproteomic analysisrecellularization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tadeu Ériton Caliman Zanardo Tadeu Ériton Caliman Zanardo Fernanda Gobbi Amorim Fernanda Gobbi Amorim Gabriel Henrique Taufner Gabriel Henrique Taufner Rayssa Helena Arruda Pereira Rayssa Helena Arruda Pereira Ian Manhoni Baiense Afrânio Côgo Destefani Afrânio Côgo Destefani Leo Kei Iwai Raul Cavalcante Maranhão Breno Valentim Nogueira Breno Valentim Nogueira |
spellingShingle |
Tadeu Ériton Caliman Zanardo Tadeu Ériton Caliman Zanardo Fernanda Gobbi Amorim Fernanda Gobbi Amorim Gabriel Henrique Taufner Gabriel Henrique Taufner Rayssa Helena Arruda Pereira Rayssa Helena Arruda Pereira Ian Manhoni Baiense Afrânio Côgo Destefani Afrânio Côgo Destefani Leo Kei Iwai Raul Cavalcante Maranhão Breno Valentim Nogueira Breno Valentim Nogueira Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering Frontiers in Bioengineering and Biotechnology splenic scaffold decellularization extracellular matrix proteomic analysis recellularization |
author_facet |
Tadeu Ériton Caliman Zanardo Tadeu Ériton Caliman Zanardo Fernanda Gobbi Amorim Fernanda Gobbi Amorim Gabriel Henrique Taufner Gabriel Henrique Taufner Rayssa Helena Arruda Pereira Rayssa Helena Arruda Pereira Ian Manhoni Baiense Afrânio Côgo Destefani Afrânio Côgo Destefani Leo Kei Iwai Raul Cavalcante Maranhão Breno Valentim Nogueira Breno Valentim Nogueira |
author_sort |
Tadeu Ériton Caliman Zanardo |
title |
Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering |
title_short |
Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering |
title_full |
Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering |
title_fullStr |
Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering |
title_full_unstemmed |
Decellularized Splenic Matrix as a Scaffold for Spleen Bioengineering |
title_sort |
decellularized splenic matrix as a scaffold for spleen bioengineering |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2020-10-01 |
description |
The spleen is considered a non-essential organ. However, its importance is increasingly clear, given the serious disorders caused by its absence or dysfunction, e.g., greater susceptibility to infections, thromboembolism and cancer. Surgical techniques to preserve the spleen and maintain splenic function have become increasingly common. However, the morbidity and mortality associated with its absence and dysfunction are still high. We used the decellularization technique to obtain a viable splenic scaffold for recellularization in vitro and propose the idea of bioengineered spleen transplantation to the host. We observed the maintenance of important structural components such as white pulp, marginal zone and red pulp, in addition to the network of vascular ducts. The decellularized scaffold presents minimal residual DNA and SDS, which are essential to prevent immunogenic responses and transplantation failure. Also, the main components of the splenic matrix were preserved after decellularization, with retention of approximately 72% in the matrisomal protein content. The scaffold we developed was partially recellularized with stromal cells from the spleen of neonatal rats, demonstrating adhesion, proliferation and viability of cells. Therefore, the splenic scaffold is very promising for use in studies on spleen reconstruction and transplantation, with the aim of complete recovery of splenic function. |
topic |
splenic scaffold decellularization extracellular matrix proteomic analysis recellularization |
url |
https://www.frontiersin.org/article/10.3389/fbioe.2020.573461/full |
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