Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice
Abstract Background Rice is a drought susceptible crop. A symbiotic association between rice and mycorrhizal fungi could effectively protect the plant against sudden or frequent episodes of drought. Due to its extensive network of hyphae, the endophyte is able to deeply explore the soil and transfer...
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doaj-55a31639354b4c0580efa330a48bf97a2020-11-24T22:17:20ZengSpringerOpenRice1939-84251939-84332018-05-0111111210.1186/s12284-018-0226-1Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in riceMuhammad Abu Bakar Saddique0Zulfiqar Ali1Abdus Salam Khan2Iqrar Ahmad Rana3Imran Haider Shamsi4Department of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureDepartment of Plant Breeding and Genetics, University of AgricultureCentre of Agricultural Biochemistry and Biotechnology, University of AgricultureDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang UniversityAbstract Background Rice is a drought susceptible crop. A symbiotic association between rice and mycorrhizal fungi could effectively protect the plant against sudden or frequent episodes of drought. Due to its extensive network of hyphae, the endophyte is able to deeply explore the soil and transfer water and minerals to the plant, some of them playing an important role in mitigating the effects of drought stress. Moreover, the endophyte could modify the expression of drought responsive genes and regulate antioxidants. Results Three rice genotypes, WC-297 (drought tolerant), Caawa (moderately drought tolerant) and IR-64 (drought susceptible) were inoculated with Piriformospora indica (P. indica), a dynamic endophyte. After 20 days of co-cultivation with the fungus, rice seedlings were subjected to 15% polyethylene glycol-6000 induced osmotic stress. P. indica improved the growth of rice seedlings. It alleviated the destructive effects of the applied osmotic stress. This symbiotic association increased seedling biomass, the uptake of phosphorus and zinc, which are functional elements for rice growth under drought stress. It boosted the chlorophyll fluorescence, increased the production of proline and improved the total antioxidant capacity in leaves. The association with the endophyte also up regulated the activity of the Pyrroline-5-carboxylate synthase (P5CS), which is critical for the synthesis of proline. Conclusion A mycorrhizal association between P. indica and rice seedlings provided a multifaceted protection to rice plants under osmotic stress (− 0.295 MPa).http://link.springer.com/article/10.1186/s12284-018-0226-1Oryza sativaPiriformospora indicaSymbiosisOsmotic stressFv/FmPhosphorus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Abu Bakar Saddique Zulfiqar Ali Abdus Salam Khan Iqrar Ahmad Rana Imran Haider Shamsi |
spellingShingle |
Muhammad Abu Bakar Saddique Zulfiqar Ali Abdus Salam Khan Iqrar Ahmad Rana Imran Haider Shamsi Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice Rice Oryza sativa Piriformospora indica Symbiosis Osmotic stress Fv/Fm Phosphorus |
author_facet |
Muhammad Abu Bakar Saddique Zulfiqar Ali Abdus Salam Khan Iqrar Ahmad Rana Imran Haider Shamsi |
author_sort |
Muhammad Abu Bakar Saddique |
title |
Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
title_short |
Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
title_full |
Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
title_fullStr |
Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
title_full_unstemmed |
Inoculation with the endophyte Piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
title_sort |
inoculation with the endophyte piriformospora indica significantly affects mechanisms involved in osmotic stress in rice |
publisher |
SpringerOpen |
series |
Rice |
issn |
1939-8425 1939-8433 |
publishDate |
2018-05-01 |
description |
Abstract Background Rice is a drought susceptible crop. A symbiotic association between rice and mycorrhizal fungi could effectively protect the plant against sudden or frequent episodes of drought. Due to its extensive network of hyphae, the endophyte is able to deeply explore the soil and transfer water and minerals to the plant, some of them playing an important role in mitigating the effects of drought stress. Moreover, the endophyte could modify the expression of drought responsive genes and regulate antioxidants. Results Three rice genotypes, WC-297 (drought tolerant), Caawa (moderately drought tolerant) and IR-64 (drought susceptible) were inoculated with Piriformospora indica (P. indica), a dynamic endophyte. After 20 days of co-cultivation with the fungus, rice seedlings were subjected to 15% polyethylene glycol-6000 induced osmotic stress. P. indica improved the growth of rice seedlings. It alleviated the destructive effects of the applied osmotic stress. This symbiotic association increased seedling biomass, the uptake of phosphorus and zinc, which are functional elements for rice growth under drought stress. It boosted the chlorophyll fluorescence, increased the production of proline and improved the total antioxidant capacity in leaves. The association with the endophyte also up regulated the activity of the Pyrroline-5-carboxylate synthase (P5CS), which is critical for the synthesis of proline. Conclusion A mycorrhizal association between P. indica and rice seedlings provided a multifaceted protection to rice plants under osmotic stress (− 0.295 MPa). |
topic |
Oryza sativa Piriformospora indica Symbiosis Osmotic stress Fv/Fm Phosphorus |
url |
http://link.springer.com/article/10.1186/s12284-018-0226-1 |
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