Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications
Background. While cancer/testis antigens (CTAs) are restricted in postnatal tissues to testes and germ line-derived cells, their role in cancer development and the clinical significance of their expression still remain to be better defined. Objective. The aim of this study was to investigate the lev...
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doaj-55b54937e8cb41478ddab03db13211be2020-11-24T22:25:19ZengHindawi LimitedDisease Markers0278-02401875-86302016-01-01201610.1155/2016/19875051987505Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic ImplicationsMaciej Tarnowski0Michał Czerewaty1Anna Deskur2Krzysztof Safranow3Wojciech Marlicz4Elżbieta Urasińska5Mariusz Z. Ratajczak6Teresa Starzyńska7Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Physiology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Gastroenterology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Gastroenterology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Pathomorphology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Physiology, Pomeranian Medical University, 70-111 Szczecin, PolandDepartment of Gastroenterology, Pomeranian Medical University, 70-111 Szczecin, PolandBackground. While cancer/testis antigens (CTAs) are restricted in postnatal tissues to testes and germ line-derived cells, their role in cancer development and the clinical significance of their expression still remain to be better defined. Objective. The aim of this study was to investigate the level of CTA expression in colon samples from patients with colorectal cancer (CRC) in relation to patient clinical status. Methods. Forty-five patients with newly diagnosed colorectal cancer were included in the study. We selected a panel of 18 CTAs that were previously detected in CRC as well as some new gene candidates, and their expression was detected at the mRNA level by employing RQ-PCR. Additionally, we evaluated CTA expression in three colon cancer cell lines (CL-188, HTB-39, and HTB-37) after exposure to the DNA methylation-modifying drug 5-azacytidine. Results. We report that 6 out of 18 (33%) CTAs tested (MAGEA3, OIP5, TTK, PLU1, DKKL1, and FBXO39) were significantly (p<0.05) overexpressed in tumor tissue compared with healthy colon samples isolated from the same patients. Conclusions. Moreover, we found that MAGEA3, PLU-1, and DKKL expression positively correlated with disease progression, evaluated according to the Dukes staging system. Finally, 5-azacytidine exposure significantly upregulated expression of CTAs on CRC cells, which indicates that this demethylation agent could be employed therapeutically to enhance the immune response against tumor cells.http://dx.doi.org/10.1155/2016/1987505 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maciej Tarnowski Michał Czerewaty Anna Deskur Krzysztof Safranow Wojciech Marlicz Elżbieta Urasińska Mariusz Z. Ratajczak Teresa Starzyńska |
spellingShingle |
Maciej Tarnowski Michał Czerewaty Anna Deskur Krzysztof Safranow Wojciech Marlicz Elżbieta Urasińska Mariusz Z. Ratajczak Teresa Starzyńska Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications Disease Markers |
author_facet |
Maciej Tarnowski Michał Czerewaty Anna Deskur Krzysztof Safranow Wojciech Marlicz Elżbieta Urasińska Mariusz Z. Ratajczak Teresa Starzyńska |
author_sort |
Maciej Tarnowski |
title |
Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications |
title_short |
Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications |
title_full |
Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications |
title_fullStr |
Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications |
title_full_unstemmed |
Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications |
title_sort |
expression of cancer testis antigens in colorectal cancer: new prognostic and therapeutic implications |
publisher |
Hindawi Limited |
series |
Disease Markers |
issn |
0278-0240 1875-8630 |
publishDate |
2016-01-01 |
description |
Background. While cancer/testis antigens (CTAs) are restricted in postnatal tissues to testes and germ line-derived cells, their role in cancer development and the clinical significance of their expression still remain to be better defined. Objective. The aim of this study was to investigate the level of CTA expression in colon samples from patients with colorectal cancer (CRC) in relation to patient clinical status. Methods. Forty-five patients with newly diagnosed colorectal cancer were included in the study. We selected a panel of 18 CTAs that were previously detected in CRC as well as some new gene candidates, and their expression was detected at the mRNA level by employing RQ-PCR. Additionally, we evaluated CTA expression in three colon cancer cell lines (CL-188, HTB-39, and HTB-37) after exposure to the DNA methylation-modifying drug 5-azacytidine. Results. We report that 6 out of 18 (33%) CTAs tested (MAGEA3, OIP5, TTK, PLU1, DKKL1, and FBXO39) were significantly (p<0.05) overexpressed in tumor tissue compared with healthy colon samples isolated from the same patients. Conclusions. Moreover, we found that MAGEA3, PLU-1, and DKKL expression positively correlated with disease progression, evaluated according to the Dukes staging system. Finally, 5-azacytidine exposure significantly upregulated expression of CTAs on CRC cells, which indicates that this demethylation agent could be employed therapeutically to enhance the immune response against tumor cells. |
url |
http://dx.doi.org/10.1155/2016/1987505 |
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