Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG
Summary The preservation of the viability of microorganisms in probiotic formulations is the most important parameter ensuring the adequate concentration of live microorganisms at the time of administration. The formulation and processing techniques used to produce these probiotic formulations can i...
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doaj-55b5821c5d6f4033b12e048169caf8332020-11-25T02:57:49ZengWileyMicrobial Biotechnology1751-79152019-09-0112584985510.1111/1751-7915.13426Impact of spray‐drying on the pili of Lactobacillus rhamnosus GGShari Kiekens0Dieter Vandenheuvel1Géraldine Broeckx2Ingmar Claes3Camille Allonsius4Ilke De Boeck5Sofie Thys6Jean‐Pierre Timmermans7Filip Kiekens8Sarah Lebeer9Department of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumDepartment of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumLaboratory of Pharmaceutical Technology and Biopharmacy Department of Pharmaceutical, Biomedical and Veterinary Sciences University of Antwerp Universiteitsplein 1 B‐2610 Wilrijk BelgiumDepartment of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumDepartment of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumDepartment of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumLaboratory of Cell Biology and Histology Department of Pharmaceutical, Biomedical and Veterinary Sciences Antwerp Centre for Advanced Microscopy (ACAM) University of Antwerp Universiteitsplein 1 B‐2610 Wilrijk BelgiumLaboratory of Cell Biology and Histology Department of Pharmaceutical, Biomedical and Veterinary Sciences Antwerp Centre for Advanced Microscopy (ACAM) University of Antwerp Universiteitsplein 1 B‐2610 Wilrijk BelgiumLaboratory of Pharmaceutical Technology and Biopharmacy Department of Pharmaceutical, Biomedical and Veterinary Sciences University of Antwerp Universiteitsplein 1 B‐2610 Wilrijk BelgiumDepartment of Bioscience Engineering Research Group Environmental Ecology and Applied Microbiology University of Antwerp Groenenborgerlaan 171 B‐2020 Antwerp BelgiumSummary The preservation of the viability of microorganisms in probiotic formulations is the most important parameter ensuring the adequate concentration of live microorganisms at the time of administration. The formulation and processing techniques used to produce these probiotic formulations can influence the preservation of the microbial viability. However, it is also required that the bacteria maintain their key probiotic capacities during processing, formulation and shelf life. In this study, we investigated the impact of spray‐drying on different cell wall properties of the model probiotic strain Lactobacillus rhamnosus GG, including its adherence to intestinal epithelial cells. The dltD gene knock‐out mutant, L. rhamnosus GG CMPG5540, displaying modified cell wall lipoteichoic acids, showed significantly increased colony‐forming units after spray‐drying and subsequent storage under standard conditions compared to wild‐type L. rhamnosus GG. In contrast, disruption of the biosynthesis of exopolysaccharides or pili expression did not impact survival. However, spray‐drying did significantly affect the adherence capacity of L. rhamnosus GG. Scanning electron microscopy confirmed that the pili, key surface factors for adherence to intestinal cells and mucus, were sheared off during the spray‐drying process. These data thus highlight that both the functionality and viability of probiotics should be assessed during the spray‐drying process and subsequent storage.https://doi.org/10.1111/1751-7915.13426 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shari Kiekens Dieter Vandenheuvel Géraldine Broeckx Ingmar Claes Camille Allonsius Ilke De Boeck Sofie Thys Jean‐Pierre Timmermans Filip Kiekens Sarah Lebeer |
spellingShingle |
Shari Kiekens Dieter Vandenheuvel Géraldine Broeckx Ingmar Claes Camille Allonsius Ilke De Boeck Sofie Thys Jean‐Pierre Timmermans Filip Kiekens Sarah Lebeer Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG Microbial Biotechnology |
author_facet |
Shari Kiekens Dieter Vandenheuvel Géraldine Broeckx Ingmar Claes Camille Allonsius Ilke De Boeck Sofie Thys Jean‐Pierre Timmermans Filip Kiekens Sarah Lebeer |
author_sort |
Shari Kiekens |
title |
Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG |
title_short |
Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG |
title_full |
Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG |
title_fullStr |
Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG |
title_full_unstemmed |
Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG |
title_sort |
impact of spray‐drying on the pili of lactobacillus rhamnosus gg |
publisher |
Wiley |
series |
Microbial Biotechnology |
issn |
1751-7915 |
publishDate |
2019-09-01 |
description |
Summary The preservation of the viability of microorganisms in probiotic formulations is the most important parameter ensuring the adequate concentration of live microorganisms at the time of administration. The formulation and processing techniques used to produce these probiotic formulations can influence the preservation of the microbial viability. However, it is also required that the bacteria maintain their key probiotic capacities during processing, formulation and shelf life. In this study, we investigated the impact of spray‐drying on different cell wall properties of the model probiotic strain Lactobacillus rhamnosus GG, including its adherence to intestinal epithelial cells. The dltD gene knock‐out mutant, L. rhamnosus GG CMPG5540, displaying modified cell wall lipoteichoic acids, showed significantly increased colony‐forming units after spray‐drying and subsequent storage under standard conditions compared to wild‐type L. rhamnosus GG. In contrast, disruption of the biosynthesis of exopolysaccharides or pili expression did not impact survival. However, spray‐drying did significantly affect the adherence capacity of L. rhamnosus GG. Scanning electron microscopy confirmed that the pili, key surface factors for adherence to intestinal cells and mucus, were sheared off during the spray‐drying process. These data thus highlight that both the functionality and viability of probiotics should be assessed during the spray‐drying process and subsequent storage. |
url |
https://doi.org/10.1111/1751-7915.13426 |
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