Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel
An attractive strategy for the regeneration of tissues has been the use of extracellular matrix analogous biomaterials. Peptide-based fibrillar hydrogels have been shown to mimic the structure of extracellular matrix offering cells a niche to undertake their physiological functions. In this study, t...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2016-07-01
|
Series: | Journal of Tissue Engineering |
Online Access: | https://doi.org/10.1177/2041731416649789 |
id |
doaj-55de044adc4b43639bd380d3726e3496 |
---|---|
record_format |
Article |
spelling |
doaj-55de044adc4b43639bd380d3726e34962020-11-25T03:40:12ZengSAGE PublishingJournal of Tissue Engineering2041-73142016-07-01710.1177/204173141664978910.1177_2041731416649789Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogelLuis A Castillo Diaz0Mohamed Elsawy1Alberto Saiani2Julie E Gough3Aline F Miller4Manchester Institute of Biotechnology, The University of Manchester, Manchester, UKManchester Institute of Biotechnology, The University of Manchester, Manchester, UKThe School of Materials, The University of Manchester, Manchester, UKThe School of Materials, The University of Manchester, Manchester, UKManchester Institute of Biotechnology, The University of Manchester, Manchester, UKAn attractive strategy for the regeneration of tissues has been the use of extracellular matrix analogous biomaterials. Peptide-based fibrillar hydrogels have been shown to mimic the structure of extracellular matrix offering cells a niche to undertake their physiological functions. In this study, the capability of an ionic-complementary peptide FEFEFKFK (F, E, and K are phenylalanine, glutamic acid, and lysine, respectively) hydrogel to host human mesenchymal stem cells in three dimensions and induce their osteogenic differentiation is demonstrated. Assays showed sustained cell viability and proliferation throughout the hydrogel over 12 days of culture and these human mesenchymal stem cells differentiated into osteoblasts simply upon addition of osteogenic stimulation. Differentiated osteoblasts synthesized key bone proteins, including collagen-1 (Col-1), osteocalcin, and alkaline phosphatase. Moreover, mineralization occurred within the hydrogel. The peptide hydrogel is a naturally biodegradable material as shown by oscillatory rheology and reversed-phase high-performance liquid chromatography, where both viscoelastic properties and the degradation of the hydrogel were monitored over time, respectively. These findings demonstrate that a biodegradable octapeptide hydrogel can host and induce the differentiation of stem cells and has the potential for the regeneration of hard tissues such as alveolar bone.https://doi.org/10.1177/2041731416649789 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luis A Castillo Diaz Mohamed Elsawy Alberto Saiani Julie E Gough Aline F Miller |
spellingShingle |
Luis A Castillo Diaz Mohamed Elsawy Alberto Saiani Julie E Gough Aline F Miller Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel Journal of Tissue Engineering |
author_facet |
Luis A Castillo Diaz Mohamed Elsawy Alberto Saiani Julie E Gough Aline F Miller |
author_sort |
Luis A Castillo Diaz |
title |
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
title_short |
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
title_full |
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
title_fullStr |
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
title_full_unstemmed |
Osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
title_sort |
osteogenic differentiation of human mesenchymal stem cells promotes mineralization within a biodegradable peptide hydrogel |
publisher |
SAGE Publishing |
series |
Journal of Tissue Engineering |
issn |
2041-7314 |
publishDate |
2016-07-01 |
description |
An attractive strategy for the regeneration of tissues has been the use of extracellular matrix analogous biomaterials. Peptide-based fibrillar hydrogels have been shown to mimic the structure of extracellular matrix offering cells a niche to undertake their physiological functions. In this study, the capability of an ionic-complementary peptide FEFEFKFK (F, E, and K are phenylalanine, glutamic acid, and lysine, respectively) hydrogel to host human mesenchymal stem cells in three dimensions and induce their osteogenic differentiation is demonstrated. Assays showed sustained cell viability and proliferation throughout the hydrogel over 12 days of culture and these human mesenchymal stem cells differentiated into osteoblasts simply upon addition of osteogenic stimulation. Differentiated osteoblasts synthesized key bone proteins, including collagen-1 (Col-1), osteocalcin, and alkaline phosphatase. Moreover, mineralization occurred within the hydrogel. The peptide hydrogel is a naturally biodegradable material as shown by oscillatory rheology and reversed-phase high-performance liquid chromatography, where both viscoelastic properties and the degradation of the hydrogel were monitored over time, respectively. These findings demonstrate that a biodegradable octapeptide hydrogel can host and induce the differentiation of stem cells and has the potential for the regeneration of hard tissues such as alveolar bone. |
url |
https://doi.org/10.1177/2041731416649789 |
work_keys_str_mv |
AT luisacastillodiaz osteogenicdifferentiationofhumanmesenchymalstemcellspromotesmineralizationwithinabiodegradablepeptidehydrogel AT mohamedelsawy osteogenicdifferentiationofhumanmesenchymalstemcellspromotesmineralizationwithinabiodegradablepeptidehydrogel AT albertosaiani osteogenicdifferentiationofhumanmesenchymalstemcellspromotesmineralizationwithinabiodegradablepeptidehydrogel AT julieegough osteogenicdifferentiationofhumanmesenchymalstemcellspromotesmineralizationwithinabiodegradablepeptidehydrogel AT alinefmiller osteogenicdifferentiationofhumanmesenchymalstemcellspromotesmineralizationwithinabiodegradablepeptidehydrogel |
_version_ |
1724535596620185600 |