Downregulation of circRNA_0000285 Suppresses Cervical Cancer Development by Regulating miR197-3p–ELK1 Axis

• Main Authors: Zhang W, Zhang S
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-09-01
• Series: Cancer Management and Research
• Subjects: cervical cancer; circ_0000285; mir-197-3p; elk1
• Online Access: https://www.dovepress.com/downregulation-of-circrna0000285-suppresses-cervical-cancer-developmen-peer-reviewed-article-CMAR

Wenmin Zhang,1 Suping Zhang2 1Department of Obstetrics and Gynecology, Heze Municipal Hospital, Heze, Shandong 274000, People’s Republic of China; 2Department of Reproductive, Zoucheng People’s Hospital, Zoucheng, Shandong 273500, People’s Republic of ChinaCorrespondence: Suping Zhang Tel [phone]Email [e-mail]: Circular RNAs (circRNAs) are involved in the development of human cancers, including cervical cancer (CC). However, the role and mechanism of the circRNA hsa_circ_0000285 (circ_0000285) in CC development remain largely unknown.Methods: Thirty paired CC and adjacent normal tissue samples were harvested. CC cell lines SiHa and HeLa were cultured in this study. The expression of circ_0000285, miR197-3p and ELK1 was detected via qRT-PCR or Western blot. CC development was assessed via cell viability, colony formation, apoptosis, cell cycle, and autophagy using MTT, colony-formation assays, flow cytometry and Western blot. The target association was analyzed via dual luciferase–reporter assay, RNA immunoprecipitation, and RNA pull-down. The role of circ_0000285 in CC in vivo was analyzed using a xenograft model.Results: circ_0000285 abundance was enhanced in CC tissue and cells and mainly located in cytoplasm. Silence of circ_0000285 suppressed cell viability and colony formation, arrested the cell cycle at the G0/G1 phase, and induced apoptosis and autophagy in CC cells. miR197-3p was targeted by circ_0000285, and miR197-3p knockdown reversed the effect of circ_0000285 silence on CC development. miR197-3p directly targeted ELK1 to inhibit CC development. circ_0000285 regulated ELK1 by modulating miR197-3p. Knockdown of circ_0000285 reduced xenograft tumor growth in vivo.Conclusion: Knockdown of circ_0000285 repressed CC development by increasing miR197-3p and decreasing ELK1.Keywords: cervical cancer, circ_0000285, miR197-3p, ELK1