Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells
Nanoclays have potential applications in biomedicine raising the need to evaluate their toxicity in in vitro models as a first approach to its biocompatibility. In this study, in vitro toxicity of clinoptilolite and sepiolite nanoclays (NC) was analyzed in highly phagocytic cultures of amoebas and h...
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doaj-55ebfc78da964d8f956daa2d9c2aac8e2020-11-24T23:04:31ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/164980164980Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic CellsYanis Toledano-Magaña0Leticia Flores-Santos1Georgina Montes de Oca2Alfonso González-Montiel3Juan-Pedro Laclette4Julio-César Carrero5Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, MexicoCentro de Investigación y Desarrollo Tecnológico, S.A. de C.V., Avenida de los Sauces No. 87, Mz 6, Parque Industrial Lerma, 52000 Toluca, MexicoCentro de Investigación y Desarrollo Tecnológico, S.A. de C.V., Avenida de los Sauces No. 87, Mz 6, Parque Industrial Lerma, 52000 Toluca, MexicoCentro de Investigación y Desarrollo Tecnológico, S.A. de C.V., Avenida de los Sauces No. 87, Mz 6, Parque Industrial Lerma, 52000 Toluca, MexicoDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, MexicoDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 México, DF, MexicoNanoclays have potential applications in biomedicine raising the need to evaluate their toxicity in in vitro models as a first approach to its biocompatibility. In this study, in vitro toxicity of clinoptilolite and sepiolite nanoclays (NC) was analyzed in highly phagocytic cultures of amoebas and human and mice macrophages. While amebic viability was significantly affected only by sepiolite NC at concentrations higher than 0.1 mg/mL, the effect on macrophage cultures was dependent on the origin of the cells. Macrophages derived from human peripheral blood monocytes were less affected in viability (25% decrease at 48 h), followed by the RAW 264.7 cell line (40%), and finally, macrophages derived from mice bone marrow monocytes (98%). Moreover, the cell line and mice macrophages die mainly by necrosis, whereas human macrophages exhibit increased apoptosis. Cytokine expression analysis in media of sepiolite NC treated cultures showed a proinflammatory profile (INFγ, IL-1α, IL-8, and IL-6), in contrast with clinoptilolite NC that induced lees cytokines with concomitant production of IL-10. The results show that sepiolite NC is more toxic to amoebas and macrophages than clinoptilolite NC, mostly in a time and dose-dependent manner. However, the effect of sepiolite NC was comparable with talc powder suggesting that both NC have low cytotoxicity in vitro.http://dx.doi.org/10.1155/2015/164980 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanis Toledano-Magaña Leticia Flores-Santos Georgina Montes de Oca Alfonso González-Montiel Juan-Pedro Laclette Julio-César Carrero |
spellingShingle |
Yanis Toledano-Magaña Leticia Flores-Santos Georgina Montes de Oca Alfonso González-Montiel Juan-Pedro Laclette Julio-César Carrero Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells BioMed Research International |
author_facet |
Yanis Toledano-Magaña Leticia Flores-Santos Georgina Montes de Oca Alfonso González-Montiel Juan-Pedro Laclette Julio-César Carrero |
author_sort |
Yanis Toledano-Magaña |
title |
Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells |
title_short |
Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells |
title_full |
Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells |
title_fullStr |
Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells |
title_full_unstemmed |
Effect of Clinoptilolite and Sepiolite Nanoclays on Human and Parasitic Highly Phagocytic Cells |
title_sort |
effect of clinoptilolite and sepiolite nanoclays on human and parasitic highly phagocytic cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Nanoclays have potential applications in biomedicine raising the need to evaluate their toxicity in in vitro models as a first approach to its biocompatibility. In this study, in vitro toxicity of clinoptilolite and sepiolite nanoclays (NC) was analyzed in highly phagocytic cultures of amoebas and human and mice macrophages. While amebic viability was significantly affected only by sepiolite NC at concentrations higher than 0.1 mg/mL, the effect on macrophage cultures was dependent on the origin of the cells. Macrophages derived from human peripheral blood monocytes were less affected in viability (25% decrease at 48 h), followed by the RAW 264.7 cell line (40%), and finally, macrophages derived from mice bone marrow monocytes (98%). Moreover, the cell line and mice macrophages die mainly by necrosis, whereas human macrophages exhibit increased apoptosis. Cytokine expression analysis in media of sepiolite NC treated cultures showed a proinflammatory profile (INFγ, IL-1α, IL-8, and IL-6), in contrast with clinoptilolite NC that induced lees cytokines with concomitant production of IL-10. The results show that sepiolite NC is more toxic to amoebas and macrophages than clinoptilolite NC, mostly in a time and dose-dependent manner. However, the effect of sepiolite NC was comparable with talc powder suggesting that both NC have low cytotoxicity in vitro. |
url |
http://dx.doi.org/10.1155/2015/164980 |
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