Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors
Conventional dendritic cells (cDCs) are thought to descend from a DC precursor downstream of the common myeloid progenitor (CMP). However, a mouse lymphoid-primed multipotent progenitor has been shown to generate cDCs following a DC-specific developmental pathway independent of monocyte and granuloc...
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doaj-5606587f98b7460ca7563a94b0c49ff82020-11-25T02:32:45ZengElsevierCell Reports2211-12472017-07-0120352953710.1016/j.celrep.2017.06.075Dendritic Cell Lineage Potential in Human Early Hematopoietic ProgenitorsJulie Helft0Fernando Anjos-Afonso1Annemarthe G. van der Veen2Probir Chakravarty3Dominique Bonnet4Caetano Reis e Sousa5Immunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKImmunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKBioinformatics, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKImmunobiology Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UKConventional dendritic cells (cDCs) are thought to descend from a DC precursor downstream of the common myeloid progenitor (CMP). However, a mouse lymphoid-primed multipotent progenitor has been shown to generate cDCs following a DC-specific developmental pathway independent of monocyte and granulocyte poiesis. Similarly, here we show that, in humans, a large fraction of multipotent lymphoid early progenitors (MLPs) gives rise to cDCs, in particular the subset known as cDC1, identified by co-expression of DNGR-1 (CLEC9A) and CD141 (BDCA-3). Single-cell analysis indicates that over one-third of MLPs have the potential to efficiently generate cDCs. cDC1s generated from CMPs or MLPs do not exhibit differences in transcriptome or phenotype. These results demonstrate an early imprinting of the cDC lineage in human hematopoiesis and highlight the plasticity of developmental pathways giving rise to human DCs.http://www.sciencedirect.com/science/article/pii/S2211124717309063dendritic cellshematopoiesislymphoid cellsmyeloid cellshuman |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julie Helft Fernando Anjos-Afonso Annemarthe G. van der Veen Probir Chakravarty Dominique Bonnet Caetano Reis e Sousa |
spellingShingle |
Julie Helft Fernando Anjos-Afonso Annemarthe G. van der Veen Probir Chakravarty Dominique Bonnet Caetano Reis e Sousa Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors Cell Reports dendritic cells hematopoiesis lymphoid cells myeloid cells human |
author_facet |
Julie Helft Fernando Anjos-Afonso Annemarthe G. van der Veen Probir Chakravarty Dominique Bonnet Caetano Reis e Sousa |
author_sort |
Julie Helft |
title |
Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors |
title_short |
Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors |
title_full |
Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors |
title_fullStr |
Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors |
title_full_unstemmed |
Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors |
title_sort |
dendritic cell lineage potential in human early hematopoietic progenitors |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2017-07-01 |
description |
Conventional dendritic cells (cDCs) are thought to descend from a DC precursor downstream of the common myeloid progenitor (CMP). However, a mouse lymphoid-primed multipotent progenitor has been shown to generate cDCs following a DC-specific developmental pathway independent of monocyte and granulocyte poiesis. Similarly, here we show that, in humans, a large fraction of multipotent lymphoid early progenitors (MLPs) gives rise to cDCs, in particular the subset known as cDC1, identified by co-expression of DNGR-1 (CLEC9A) and CD141 (BDCA-3). Single-cell analysis indicates that over one-third of MLPs have the potential to efficiently generate cDCs. cDC1s generated from CMPs or MLPs do not exhibit differences in transcriptome or phenotype. These results demonstrate an early imprinting of the cDC lineage in human hematopoiesis and highlight the plasticity of developmental pathways giving rise to human DCs. |
topic |
dendritic cells hematopoiesis lymphoid cells myeloid cells human |
url |
http://www.sciencedirect.com/science/article/pii/S2211124717309063 |
work_keys_str_mv |
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