Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar
Mineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation vi...
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doaj-5619bb55dcdf4174b2b71316947208322021-08-26T14:01:20ZengMDPI AGMaterials1996-19442021-08-01144640464010.3390/ma14164640Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat MolarMaiko Yamada0Motohiko Nagayama1Yuka Miyamoto2Satoshi Kawano3Yoshiaki Takitani4Masashi Tanaka5Michiko Ehara6Juna Nakao7Takanaga Ochiai8Yoshihiro Shibukawa9Takakazu Yoshida10Department of Endodontics, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Endodontics, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Endodontics, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Endodontics, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Oral Pathology, Division of Oral Pathogenesis and Disease Control, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanDepartment of Removable Partial Prosthodontics, Tokyo Dental College, Chiyoda-Ku, Tokyo 101-0061, JapanDepartment of Endodontics, Division of Oral Functional Science and Rehabilitation, School of Dentistry, Asahi University, Mizuho, Gifu 501-0296, JapanMineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation via IPMSCs in the early phase of regeneration and compare with calcium hydroxide (CH). Direct pulp capping using calcium hydroxide (CH), MTA, and MTA with platelet-rich plasma (MTA + PRP) was performed on maxillary first molars of 8-week-old male Wistar rats (<i>n</i> = 36). After 3, 7, or 14 days, the teeth were analyzed for mineral density (MD) and volume of MD (VMD) via micro-focusing computed tomography (µCT), nestin, dentin matrix acidic phosphoprotein 1 (<i>DMP1</i>) immunohistochemistry, and real-time PCR for <i>DMP1</i> mRNA expression. MTA stimulated the early phase differentiation of the IPMSCs into odontoblasts, with positive results for nestin and <i>DMP1</i> compared with CH. Moreover, MTA + PRP stimulated calcified granule and dentin bridge formation through calcium mineral deposition, following the induction of <i>DMP1</i> mRNA expression in IPMSCs. Our results suggested that the combination of MTA and PRP is an effective and clinically applicable method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration.https://www.mdpi.com/1996-1944/14/16/4640mineral trioxide aggregatedirect pulp cappingmineral densitynestindentin matrix acidic phosphoprotein 1 (<i>DMP1</i>)dentin bridge |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maiko Yamada Motohiko Nagayama Yuka Miyamoto Satoshi Kawano Yoshiaki Takitani Masashi Tanaka Michiko Ehara Juna Nakao Takanaga Ochiai Yoshihiro Shibukawa Takakazu Yoshida |
spellingShingle |
Maiko Yamada Motohiko Nagayama Yuka Miyamoto Satoshi Kawano Yoshiaki Takitani Masashi Tanaka Michiko Ehara Juna Nakao Takanaga Ochiai Yoshihiro Shibukawa Takakazu Yoshida Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar Materials mineral trioxide aggregate direct pulp capping mineral density nestin dentin matrix acidic phosphoprotein 1 (<i>DMP1</i>) dentin bridge |
author_facet |
Maiko Yamada Motohiko Nagayama Yuka Miyamoto Satoshi Kawano Yoshiaki Takitani Masashi Tanaka Michiko Ehara Juna Nakao Takanaga Ochiai Yoshihiro Shibukawa Takakazu Yoshida |
author_sort |
Maiko Yamada |
title |
Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar |
title_short |
Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar |
title_full |
Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar |
title_fullStr |
Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar |
title_full_unstemmed |
Mineral Trioxide Aggregate (MTA) Upregulates the Expression of <i>DMP1</i> in Direct Pulp Capping in the Rat Molar |
title_sort |
mineral trioxide aggregate (mta) upregulates the expression of <i>dmp1</i> in direct pulp capping in the rat molar |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2021-08-01 |
description |
Mineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation via IPMSCs in the early phase of regeneration and compare with calcium hydroxide (CH). Direct pulp capping using calcium hydroxide (CH), MTA, and MTA with platelet-rich plasma (MTA + PRP) was performed on maxillary first molars of 8-week-old male Wistar rats (<i>n</i> = 36). After 3, 7, or 14 days, the teeth were analyzed for mineral density (MD) and volume of MD (VMD) via micro-focusing computed tomography (µCT), nestin, dentin matrix acidic phosphoprotein 1 (<i>DMP1</i>) immunohistochemistry, and real-time PCR for <i>DMP1</i> mRNA expression. MTA stimulated the early phase differentiation of the IPMSCs into odontoblasts, with positive results for nestin and <i>DMP1</i> compared with CH. Moreover, MTA + PRP stimulated calcified granule and dentin bridge formation through calcium mineral deposition, following the induction of <i>DMP1</i> mRNA expression in IPMSCs. Our results suggested that the combination of MTA and PRP is an effective and clinically applicable method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration. |
topic |
mineral trioxide aggregate direct pulp capping mineral density nestin dentin matrix acidic phosphoprotein 1 (<i>DMP1</i>) dentin bridge |
url |
https://www.mdpi.com/1996-1944/14/16/4640 |
work_keys_str_mv |
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1721191814779633664 |