Rapid Propagation of Sweet and Sour Cherry Rootstocks
<p><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">The paper presents a protocol for micropropagation of <i>Prunus</i> sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversit...
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2014-12-01
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Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
Online Access: | http://www.notulaebotanicae.ro/index.php/nbha/article/view/9671 |
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doaj-562367d167144215a79aaf2e157c8eb92021-05-02T20:03:31ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092014-12-0142248849410.15835/nbha.42.2.96718002Rapid Propagation of Sweet and Sour Cherry RootstocksDušica DORIĆ0Vladislav OGNJANOV1Mirjana LJUBOJEVIĆ2Goran BARAĆ3Jovana DULIĆ4Ankica PRANJIĆ5Krunoslav DUGALIĆ6University of Novi Sad, Faculty of Agriculture, Department of Fruit Science, Viticulture, Horticulture and Landscape Architecture, Dositej Obradovic Sq. 8, 21000 Novi SadUniversity of Novi Sad, Faculty of Agriculture, Department of Fruit Science, Viticulture, Horticulture and Landscape Architecture, Dositej Obradovic Sq. 8, 21000 Novi SadUniversity of Novi Sad, Faculty of Agriculture, Department of Fruit Science, Viticulture, Horticulture and Landscape Architecture, Dositej Obradovic Sq. 8, 21000 Novi SadUniversity of Novi Sad, Faculty of Agriculture, Department of Fruit Science, Viticulture, Horticulture and Landscape Architecture, Dositej Obradovic Sq. 8, 21000 Novi SadUniversity of Novi Sad, Faculty of Agriculture, Department of Fruit Science, Viticulture, Horticulture and Landscape Architecture, Dositej Obradovic Sq. 8, 21000 Novi SadAgricultural Institute Osijek, Department of Fruit Science, Juzno predgradje 17, 31000 OsijekAgricultural Institute Osijek, Department of Fruit Science, Juzno predgradje 17, 31000 Osijek<p><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">The paper presents a protocol for micropropagation of <i>Prunus</i> sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In <i>P. cerasus</i> L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, ‘Gisela 6’. For <i>P. fruticosa</i> Pall., selection ‘SV1’ and ‘SV2’, and <i>P. cerasus</i> ‘D6’ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut) medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric) in the 200 mg l<sup>-1</sup> concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for ‘Gisela 6’ and ‘D6’ genotype at 1 mg<sup> </sup>l<sup>-1</sup> IBA (indole-3-butyric acid), while 0.8 mg<sup> </sup>l<sup>-1</sup> was the optimum concentration for <i>P. mahaleb</i> L. ‘M1’ selection. <i>P. fruticosa</i> genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l<sup>-1</sup>).</span></p>http://www.notulaebotanicae.ro/index.php/nbha/article/view/9671 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dušica DORIĆ Vladislav OGNJANOV Mirjana LJUBOJEVIĆ Goran BARAĆ Jovana DULIĆ Ankica PRANJIĆ Krunoslav DUGALIĆ |
spellingShingle |
Dušica DORIĆ Vladislav OGNJANOV Mirjana LJUBOJEVIĆ Goran BARAĆ Jovana DULIĆ Ankica PRANJIĆ Krunoslav DUGALIĆ Rapid Propagation of Sweet and Sour Cherry Rootstocks Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
author_facet |
Dušica DORIĆ Vladislav OGNJANOV Mirjana LJUBOJEVIĆ Goran BARAĆ Jovana DULIĆ Ankica PRANJIĆ Krunoslav DUGALIĆ |
author_sort |
Dušica DORIĆ |
title |
Rapid Propagation of Sweet and Sour Cherry Rootstocks |
title_short |
Rapid Propagation of Sweet and Sour Cherry Rootstocks |
title_full |
Rapid Propagation of Sweet and Sour Cherry Rootstocks |
title_fullStr |
Rapid Propagation of Sweet and Sour Cherry Rootstocks |
title_full_unstemmed |
Rapid Propagation of Sweet and Sour Cherry Rootstocks |
title_sort |
rapid propagation of sweet and sour cherry rootstocks |
publisher |
AcademicPres |
series |
Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
issn |
0255-965X 1842-4309 |
publishDate |
2014-12-01 |
description |
<p><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">The paper presents a protocol for micropropagation of <i>Prunus</i> sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In <i>P. cerasus</i> L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, ‘Gisela 6’. For <i>P. fruticosa</i> Pall., selection ‘SV1’ and ‘SV2’, and <i>P. cerasus</i> ‘D6’ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut) medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric) in the 200 mg l<sup>-1</sup> concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for ‘Gisela 6’ and ‘D6’ genotype at 1 mg<sup> </sup>l<sup>-1</sup> IBA (indole-3-butyric acid), while 0.8 mg<sup> </sup>l<sup>-1</sup> was the optimum concentration for <i>P. mahaleb</i> L. ‘M1’ selection. <i>P. fruticosa</i> genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l<sup>-1</sup>).</span></p> |
url |
http://www.notulaebotanicae.ro/index.php/nbha/article/view/9671 |
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