Sequencing and annotation of the complete mitochondrial genome of a threatened labeonine fish,

• Main Authors: Mohammad Nazrul Islam, Shirin Sultana, Md. Jobaidul Alam
• Format: Article
• Language: English
• Published: Korea Genome Organization 2020-09-01
• Series: Genomics & Informatics
• Subjects: complete mitogenome; illumina sequencing; mitochondrial dna; reba carp; threatened species
• Online Access: http://genominfo.org/upload/pdf/gi-2020-18-3-e32.pdf

The mitochondrial genome of a species is an essential resource for its effective conservation and phylogenetic studies. In this article, we present sequencing and characterization of the complete mitochondrial genome of a threatened labeonine fish, Cirrhinus reba collected from Khulna region of Bangladesh. The complete mitochondrial genome was 16,597 bp in size, which formed a circular double-stranded DNA molecule containing a total of 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes) with two non-coding regions, an origin of light strand replication (OL) and a displacement loop (D-loop), similar structure with other fishes of Teleostei. The phylogenetic tree demonstrated its close relationship with labeonine fishes. The complete mitogenome of Cirrhinus reba (GenBank no. MN862482) showed 99.96% identity to another haplotype of Cirrhinus reba (AP013325), followed by 90.18% identity with Labeo bata (AP011198).