Control of neural stem cell survival by electroactive polymer substrates.
Stem cell function is regulated by intrinsic as well as microenvironmental factors, including chemical and mechanical signals. Conducting polymer-based cell culture substrates provide a powerful tool to control both chemical and physical stimuli sensed by stem cells. Here we show that polypyrrole (P...
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doaj-562c1b614d574948ba87510373997c5f2020-11-25T02:39:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0164e1862410.1371/journal.pone.0018624Control of neural stem cell survival by electroactive polymer substrates.Vanessa LundinAnna HerlandMagnus BerggrenEdwin W H JagerAna I TeixeiraStem cell function is regulated by intrinsic as well as microenvironmental factors, including chemical and mechanical signals. Conducting polymer-based cell culture substrates provide a powerful tool to control both chemical and physical stimuli sensed by stem cells. Here we show that polypyrrole (PPy), a commonly used conducting polymer, can be tailored to modulate survival and maintenance of rat fetal neural stem cells (NSCs). NSCs cultured on PPy substrates containing different counter ions, dodecylbenzenesulfonate (DBS), tosylate (TsO), perchlorate (ClO(4)) and chloride (Cl), showed a distinct correlation between PPy counter ion and cell viability. Specifically, NSC viability was high on PPy(DBS) but low on PPy containing TsO, ClO(4) and Cl. On PPy(DBS), NSC proliferation and differentiation was comparable to standard NSC culture on tissue culture polystyrene. Electrical reduction of PPy(DBS) created a switch for neural stem cell viability, with widespread cell death upon polymer reduction. Coating the PPy(DBS) films with a gel layer composed of a basement membrane matrix efficiently prevented loss of cell viability upon polymer reduction. Here we have defined conditions for the biocompatibility of PPy substrates with NSC culture, critical for the development of devices based on conducting polymers interfacing with NSCs.http://europepmc.org/articles/PMC3073951?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vanessa Lundin Anna Herland Magnus Berggren Edwin W H Jager Ana I Teixeira |
spellingShingle |
Vanessa Lundin Anna Herland Magnus Berggren Edwin W H Jager Ana I Teixeira Control of neural stem cell survival by electroactive polymer substrates. PLoS ONE |
author_facet |
Vanessa Lundin Anna Herland Magnus Berggren Edwin W H Jager Ana I Teixeira |
author_sort |
Vanessa Lundin |
title |
Control of neural stem cell survival by electroactive polymer substrates. |
title_short |
Control of neural stem cell survival by electroactive polymer substrates. |
title_full |
Control of neural stem cell survival by electroactive polymer substrates. |
title_fullStr |
Control of neural stem cell survival by electroactive polymer substrates. |
title_full_unstemmed |
Control of neural stem cell survival by electroactive polymer substrates. |
title_sort |
control of neural stem cell survival by electroactive polymer substrates. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Stem cell function is regulated by intrinsic as well as microenvironmental factors, including chemical and mechanical signals. Conducting polymer-based cell culture substrates provide a powerful tool to control both chemical and physical stimuli sensed by stem cells. Here we show that polypyrrole (PPy), a commonly used conducting polymer, can be tailored to modulate survival and maintenance of rat fetal neural stem cells (NSCs). NSCs cultured on PPy substrates containing different counter ions, dodecylbenzenesulfonate (DBS), tosylate (TsO), perchlorate (ClO(4)) and chloride (Cl), showed a distinct correlation between PPy counter ion and cell viability. Specifically, NSC viability was high on PPy(DBS) but low on PPy containing TsO, ClO(4) and Cl. On PPy(DBS), NSC proliferation and differentiation was comparable to standard NSC culture on tissue culture polystyrene. Electrical reduction of PPy(DBS) created a switch for neural stem cell viability, with widespread cell death upon polymer reduction. Coating the PPy(DBS) films with a gel layer composed of a basement membrane matrix efficiently prevented loss of cell viability upon polymer reduction. Here we have defined conditions for the biocompatibility of PPy substrates with NSC culture, critical for the development of devices based on conducting polymers interfacing with NSCs. |
url |
http://europepmc.org/articles/PMC3073951?pdf=render |
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