dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.
Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which...
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2016-07-01
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doaj-5635e6f747d2444d9b2fe8d2e38f8b402020-11-25T02:06:06ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-07-01127e100620410.1371/journal.pgen.1006204dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis.Catarina Brás-PereiraDelphine PotierJelle JacobsStein AertsFernando CasaresFlorence JanodyProper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis.http://europepmc.org/articles/PMC4956209?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Catarina Brás-Pereira Delphine Potier Jelle Jacobs Stein Aerts Fernando Casares Florence Janody |
spellingShingle |
Catarina Brás-Pereira Delphine Potier Jelle Jacobs Stein Aerts Fernando Casares Florence Janody dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. PLoS Genetics |
author_facet |
Catarina Brás-Pereira Delphine Potier Jelle Jacobs Stein Aerts Fernando Casares Florence Janody |
author_sort |
Catarina Brás-Pereira |
title |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. |
title_short |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. |
title_full |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. |
title_fullStr |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. |
title_full_unstemmed |
dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis. |
title_sort |
dachshund potentiates hedgehog signaling during drosophila retinogenesis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2016-07-01 |
description |
Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. |
url |
http://europepmc.org/articles/PMC4956209?pdf=render |
work_keys_str_mv |
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1724935078203621376 |