Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita

Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110...

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Main Authors:	Parthiban Subramanian, Byung-Ju Oh, Vimalraj Mani, Jae Kook Lee, Chang-Muk Lee, Joon-Soo Sim, Ja Choon Koo, Bum-Soo Hahn
Format:	Article
Language:	English
Published:	MDPI AG 2017-06-01
Series:	International Journal of Molecular Sciences
Subjects:	root-knot nematode Meloidogyne incognita developmental stages metabolic profiles metabolic pathways
Online Access:	http://www.mdpi.com/1422-0067/18/7/1351

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spelling	doaj-563ee6caaa724ae4a1a118e8f31f46cd2020-11-24T21:48:17ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-06-01187135110.3390/ijms18071351ijms18071351Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognitaParthiban Subramanian0Byung-Ju Oh1Vimalraj Mani2Jae Kook Lee3Chang-Muk Lee4Joon-Soo Sim5Ja Choon Koo6Bum-Soo Hahn7Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaDivision of Science Education and Institute of Fusion Science, Chonbuk National University, Jeonju 761-756, KoreaDepartment of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, KoreaMeloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle.http://www.mdpi.com/1422-0067/18/7/1351root-knot nematodeMeloidogyne incognitadevelopmental stagesmetabolic profilesmetabolic pathways
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Parthiban Subramanian Byung-Ju Oh Vimalraj Mani Jae Kook Lee Chang-Muk Lee Joon-Soo Sim Ja Choon Koo Bum-Soo Hahn
spellingShingle	Parthiban Subramanian Byung-Ju Oh Vimalraj Mani Jae Kook Lee Chang-Muk Lee Joon-Soo Sim Ja Choon Koo Bum-Soo Hahn Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita International Journal of Molecular Sciences root-knot nematode Meloidogyne incognita developmental stages metabolic profiles metabolic pathways
author_facet	Parthiban Subramanian Byung-Ju Oh Vimalraj Mani Jae Kook Lee Chang-Muk Lee Joon-Soo Sim Ja Choon Koo Bum-Soo Hahn
author_sort	Parthiban Subramanian
title	Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_short	Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_full	Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_fullStr	Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_full_unstemmed	Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita
title_sort	differential metabolic profiles during the developmental stages of plant-parasitic nematode meloidogyne incognita
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1422-0067
publishDate	2017-06-01
description	Meloidogyne incognita is a common root-knot nematode with a wide range of plant hosts. We aimed to study the metabolites produced at each stage of the nematode life cycle to understand its development. Metabolites of Meloidogyne incognita were extracted at egg, J2, J3, J4, and female stages and 110 metabolites with available standards were quantified using CE-TOF/MS. Analyses indicated abundance of stage-specific metabolites with the exception of J3 and J4 stages which shared similar metabolic profiles. The egg stage showed increased abundance in glycolysis and energy metabolism related metabolites while the J2 metabolites are associated with tissue formation, motility, and neurotransmission. The J3 and J4 stages indicated amino acid metabolism and urea cycle- related metabolites. The female stage was characterized with polyamine synthesis, antioxidant activity, and synthesis of reproduction related metabolites. Such metabolic profiling helps us understand the dynamic physiological changes related to each developmental stage of the root-knot nematode life cycle.
topic	root-knot nematode Meloidogyne incognita developmental stages metabolic profiles metabolic pathways
url	http://www.mdpi.com/1422-0067/18/7/1351
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Differential Metabolic Profiles during the Developmental Stages of Plant-Parasitic Nematode Meloidogyne incognita

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