Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions
Arbuscular mycorrhizal fungi (AMF) play an important role in nutrient cycling processes and plant stress resistance. To evaluate the effect of Rhizophagus irregularis CD1 on plant growth promotion (PGP) and Verticillium wilt disease, the symbiotic efficiency of AMF (SEA) was first investigated over...
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doaj-5647b42e50d04920b1c1774378d58f362020-11-24T23:58:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-01-0119124110.3390/ijms19010241ijms19010241Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field ConditionsQiang Zhang0Xinpeng Gao1Yanyun Ren2Xinhua Ding3Jiajia Qiu4Ning Li5Fanchang Zeng6Zhaohui Chu7State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaJining Academy of Agricultural Sciences, Jining 272031, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaArbuscular mycorrhizal fungi (AMF) play an important role in nutrient cycling processes and plant stress resistance. To evaluate the effect of Rhizophagus irregularis CD1 on plant growth promotion (PGP) and Verticillium wilt disease, the symbiotic efficiency of AMF (SEA) was first investigated over a range of 3% to 94% in 17 cotton varieties. The high-SEA subgroup had significant PGP effects in a greenhouse. From these results, the highest-SEA variety of Lumian 1 was selected for a two-year field assay. Consistent with the performance from the greenhouse, the AMF-mediated PGP of Lumian 1 also produced significant results, including an increased plant height, stem diameter, number of petioles, and phosphorus content. Compared with the mock treatment, AMF colonization obviously inhibited the symptom development of Verticillium dahliae and more strongly elevated the expression of pathogenesis-related genes and lignin synthesis-related genes. These results suggest that AMF colonization could lead to the mycorrhiza-induced resistance (MIR) of Lumian 1 to V. dahliae. Interestingly, our results indicated that the AMF endosymbiont could directly inhibit the growth of phytopathogenic fungi including V. dahliae by releasing undefined volatiles. In summary, our results suggest that stronger effects of AMF application result from the high-SEA.http://www.mdpi.com/1422-0067/19/1/241mycorrhizal colonizationGossypium hirsutumVerticillium wiltsymbiotic efficiencyplant growth promotionresistanceantifungal activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiang Zhang Xinpeng Gao Yanyun Ren Xinhua Ding Jiajia Qiu Ning Li Fanchang Zeng Zhaohui Chu |
spellingShingle |
Qiang Zhang Xinpeng Gao Yanyun Ren Xinhua Ding Jiajia Qiu Ning Li Fanchang Zeng Zhaohui Chu Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions International Journal of Molecular Sciences mycorrhizal colonization Gossypium hirsutum Verticillium wilt symbiotic efficiency plant growth promotion resistance antifungal activity |
author_facet |
Qiang Zhang Xinpeng Gao Yanyun Ren Xinhua Ding Jiajia Qiu Ning Li Fanchang Zeng Zhaohui Chu |
author_sort |
Qiang Zhang |
title |
Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions |
title_short |
Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions |
title_full |
Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions |
title_fullStr |
Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions |
title_full_unstemmed |
Improvement of Verticillium Wilt Resistance by Applying Arbuscular Mycorrhizal Fungi to a Cotton Variety with High Symbiotic Efficiency under Field Conditions |
title_sort |
improvement of verticillium wilt resistance by applying arbuscular mycorrhizal fungi to a cotton variety with high symbiotic efficiency under field conditions |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-01-01 |
description |
Arbuscular mycorrhizal fungi (AMF) play an important role in nutrient cycling processes and plant stress resistance. To evaluate the effect of Rhizophagus irregularis CD1 on plant growth promotion (PGP) and Verticillium wilt disease, the symbiotic efficiency of AMF (SEA) was first investigated over a range of 3% to 94% in 17 cotton varieties. The high-SEA subgroup had significant PGP effects in a greenhouse. From these results, the highest-SEA variety of Lumian 1 was selected for a two-year field assay. Consistent with the performance from the greenhouse, the AMF-mediated PGP of Lumian 1 also produced significant results, including an increased plant height, stem diameter, number of petioles, and phosphorus content. Compared with the mock treatment, AMF colonization obviously inhibited the symptom development of Verticillium dahliae and more strongly elevated the expression of pathogenesis-related genes and lignin synthesis-related genes. These results suggest that AMF colonization could lead to the mycorrhiza-induced resistance (MIR) of Lumian 1 to V. dahliae. Interestingly, our results indicated that the AMF endosymbiont could directly inhibit the growth of phytopathogenic fungi including V. dahliae by releasing undefined volatiles. In summary, our results suggest that stronger effects of AMF application result from the high-SEA. |
topic |
mycorrhizal colonization Gossypium hirsutum Verticillium wilt symbiotic efficiency plant growth promotion resistance antifungal activity |
url |
http://www.mdpi.com/1422-0067/19/1/241 |
work_keys_str_mv |
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