Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
Estimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13...
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doaj-5674d497452745ceb1df0133c5d2ac592020-11-25T02:40:35ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/84752468475246Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance StudyRamakanta Rana0Manoranjan Ranjit1Madhusmita Bal2Hemant Kumar Khuntia3Sanghamitra Pati4Sri Krishna5Aparup Das6Division of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, IndiaDivision of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, IndiaDivision of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, IndiaDivision of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, IndiaDivision of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, IndiaDivision of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, IndiaDivision of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, IndiaEstimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13-propeller domain in P. falciparum field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018-January 2019. A total of 178 P. falciparum mono infections were assessed. An 849-base pair fragment encoding the Pfk13 propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance.http://dx.doi.org/10.1155/2020/8475246 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramakanta Rana Manoranjan Ranjit Madhusmita Bal Hemant Kumar Khuntia Sanghamitra Pati Sri Krishna Aparup Das |
spellingShingle |
Ramakanta Rana Manoranjan Ranjit Madhusmita Bal Hemant Kumar Khuntia Sanghamitra Pati Sri Krishna Aparup Das Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study BioMed Research International |
author_facet |
Ramakanta Rana Manoranjan Ranjit Madhusmita Bal Hemant Kumar Khuntia Sanghamitra Pati Sri Krishna Aparup Das |
author_sort |
Ramakanta Rana |
title |
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study |
title_short |
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study |
title_full |
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study |
title_fullStr |
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study |
title_full_unstemmed |
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study |
title_sort |
sequence analysis of the k13-propeller gene in artemisinin challenging plasmodium falciparum isolates from malaria endemic areas of odisha, india: a molecular surveillance study |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Estimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13-propeller domain in P. falciparum field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018-January 2019. A total of 178 P. falciparum mono infections were assessed. An 849-base pair fragment encoding the Pfk13 propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance. |
url |
http://dx.doi.org/10.1155/2020/8475246 |
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