Identification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method

• Main Authors: Razieh Jafari, Kumarss Amini, Behroz Shojaie Sadi
• Format: Article
• Language: fas
• Published: Ilam University of Medical Sciences 2019-06-01
• Series: Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
• Subjects: Escherichia coli; Multiplex- PCR; AggR; AggA; Biofilm
• Online Access: http://sjimu.medilam.ac.ir/article-1-4781-en.html

Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli.   Materials & Methods: In the present study, 60 stool samples from children with an average age of 4 to 7 years were collected and after biochemical examinations such as catalase, oxidase, and TSI environment were identified. Ethics code: IR.IAUS.REC.1397.016   Finding: The aggA gene was in 7 samples 11.6%, agg4A 9 samples 15%, aggR 5 positive 8%. It was also found that 28% of isolated isolates had at least one of the factors of aggressiveness, aggR, aggA, and agg4A.   Discussion & Conclusions: The typical EAEC strains have a high prevalence of aggR genes in isolates isolated in this region and are heterozygous according to the pattern of virulence factors. By limiting the infections of this bacterium, it is a vaccine against a strain of the pathogen.