Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes
Quartz tuning forks are extremely good resonators and their use is growing in scanning probe microscopy. Nevertheless, only a few studies on soft biological samples have been reported using these probes. In this work, we present the methodology to develop and use these nanosensors to properly work w...
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2012-04-01
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doaj-5686ba67c8bf47b184445a30a57677e62020-11-25T00:19:20ZengMDPI AGSensors1424-82202012-04-011244803481910.3390/s120404803Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork ProbesManel Puig-VidalLaura GonzalezJorge OteroQuartz tuning forks are extremely good resonators and their use is growing in scanning probe microscopy. Nevertheless, only a few studies on soft biological samples have been reported using these probes. In this work, we present the methodology to develop and use these nanosensors to properly work with biological samples. The working principles, fabrication and experimental setup are presented. The results in the nanocharacterization of different samples in different ambients are presented by using different working modes: amplitude modulation with and without the use of a Phase-Locked Loop (PLL) and frequency modulation. Pseudomonas aeruginosa bacteria are imaged in nitrogen using amplitude modulation. Microcontact printed antibodies are imaged in buffer using amplitude modulation with a PLL. Finally, metastatic cells are imaged in air using frequency modulation.http://www.mdpi.com/1424-8220/12/4/4803/tuning forkatomic force microscopynanocharacterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manel Puig-Vidal Laura Gonzalez Jorge Otero |
spellingShingle |
Manel Puig-Vidal Laura Gonzalez Jorge Otero Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes Sensors tuning fork atomic force microscopy nanocharacterization |
author_facet |
Manel Puig-Vidal Laura Gonzalez Jorge Otero |
author_sort |
Manel Puig-Vidal |
title |
Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes |
title_short |
Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes |
title_full |
Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes |
title_fullStr |
Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes |
title_full_unstemmed |
Nanocharacterization of Soft Biological Samples in Shear Mode with Quartz Tuning Fork Probes |
title_sort |
nanocharacterization of soft biological samples in shear mode with quartz tuning fork probes |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2012-04-01 |
description |
Quartz tuning forks are extremely good resonators and their use is growing in scanning probe microscopy. Nevertheless, only a few studies on soft biological samples have been reported using these probes. In this work, we present the methodology to develop and use these nanosensors to properly work with biological samples. The working principles, fabrication and experimental setup are presented. The results in the nanocharacterization of different samples in different ambients are presented by using different working modes: amplitude modulation with and without the use of a Phase-Locked Loop (PLL) and frequency modulation. Pseudomonas aeruginosa bacteria are imaged in nitrogen using amplitude modulation. Microcontact printed antibodies are imaged in buffer using amplitude modulation with a PLL. Finally, metastatic cells are imaged in air using frequency modulation. |
topic |
tuning fork atomic force microscopy nanocharacterization |
url |
http://www.mdpi.com/1424-8220/12/4/4803/ |
work_keys_str_mv |
AT manelpuigvidal nanocharacterizationofsoftbiologicalsamplesinshearmodewithquartztuningforkprobes AT lauragonzalez nanocharacterizationofsoftbiologicalsamplesinshearmodewithquartztuningforkprobes AT jorgeotero nanocharacterizationofsoftbiologicalsamplesinshearmodewithquartztuningforkprobes |
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1725372007862763520 |