Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants
Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for...
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doaj-56ae476176ac4f64a9f5177d37f745ae2020-11-25T02:21:02ZengElsevierGenomics Data2213-59602017-06-0112C13013110.1016/j.gdata.2017.05.003Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plantsMuhammad Mu'izzuddin ZulkapliMuhammad Aqil Fitri RosliFaris Imadi Mohd SallehNormah Mohd NoorWan Mohd AizatHoe-Han GohTropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana).http://www.sciencedirect.com/science/article/pii/S2213596017300697Carnivorous plantIso-SeqNepenthesPitcherPacBio SMRTTranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Mu'izzuddin Zulkapli Muhammad Aqil Fitri Rosli Faris Imadi Mohd Salleh Normah Mohd Noor Wan Mohd Aizat Hoe-Han Goh |
spellingShingle |
Muhammad Mu'izzuddin Zulkapli Muhammad Aqil Fitri Rosli Faris Imadi Mohd Salleh Normah Mohd Noor Wan Mohd Aizat Hoe-Han Goh Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants Genomics Data Carnivorous plant Iso-Seq Nepenthes Pitcher PacBio SMRT Transcriptome |
author_facet |
Muhammad Mu'izzuddin Zulkapli Muhammad Aqil Fitri Rosli Faris Imadi Mohd Salleh Normah Mohd Noor Wan Mohd Aizat Hoe-Han Goh |
author_sort |
Muhammad Mu'izzuddin Zulkapli |
title |
Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants |
title_short |
Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants |
title_full |
Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants |
title_fullStr |
Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants |
title_full_unstemmed |
Iso-Seq analysis of Nepenthes ampullaria, Nepenthes rafflesiana and Nepenthes × hookeriana for hybridisation study in pitcher plants |
title_sort |
iso-seq analysis of nepenthes ampullaria, nepenthes rafflesiana and nepenthes × hookeriana for hybridisation study in pitcher plants |
publisher |
Elsevier |
series |
Genomics Data |
issn |
2213-5960 |
publishDate |
2017-06-01 |
description |
Tropical pitcher plants in the species-rich Nepenthaceae family of carnivorous plants possess unique pitcher organs. Hybridisation, natural or artificial, in this family is extensive resulting in pitchers with diverse features. The pitcher functions as a passive insect trap with digestive fluid for nutrient acquisition in nitrogen-poor habitats. This organ shows specialisation according to the dietary habit of different Nepenthes species. In this study, we performed the first single-molecule real-time isoform sequencing (Iso-Seq) analysis of full-length cDNA from Nepenthes ampullaria which can feed on leaf litter, compared to carnivorous Nepenthes rafflesiana, and their carnivorous hybrid Nepenthes × hookeriana. This allows the comparison of pitcher transcriptomes from the parents and the hybrid to understand how hybridisation could shape the evolution of dietary habit in Nepenthes. Raw reads have been deposited to SRA database with the accession numbers SRX2692198 (N. ampullaria), SRX2692197 (N. rafflesiana), and SRX2692196 (N. × hookeriana). |
topic |
Carnivorous plant Iso-Seq Nepenthes Pitcher PacBio SMRT Transcriptome |
url |
http://www.sciencedirect.com/science/article/pii/S2213596017300697 |
work_keys_str_mv |
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