In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses
Abstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on...
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Colégio Brasileiro de Parasitologia Veterinaria
2017-11-01
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doaj-56b774b32efb4ae2813cb463441d8f7b2020-11-25T01:15:19ZengColégio Brasileiro de Parasitologia Veterinaria Revista Brasileira de Parasitologia Veterinária1984-29612017-11-01271919410.1590/s1984-29612017055S1984-29612018000100091In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horsesMarcelo Beltrão MolentoRicardo José CaneverAbstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100091&lng=en&tlng=enCavalosanti-helmínticosteste in vitroparasitos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marcelo Beltrão Molento Ricardo José Canever |
spellingShingle |
Marcelo Beltrão Molento Ricardo José Canever In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses Revista Brasileira de Parasitologia Veterinária Cavalos anti-helmínticos teste in vitro parasitos |
author_facet |
Marcelo Beltrão Molento Ricardo José Canever |
author_sort |
Marcelo Beltrão Molento |
title |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
title_short |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
title_full |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
title_fullStr |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
title_full_unstemmed |
In vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
title_sort |
in vitro evaluation of ivermectin, moxidectin, albendazole and pyrantel against cyathostomins of horses |
publisher |
Colégio Brasileiro de Parasitologia Veterinaria |
series |
Revista Brasileira de Parasitologia Veterinária |
issn |
1984-2961 |
publishDate |
2017-11-01 |
description |
Abstract Cyathostomins are the most prevalent nematodes of horses, and multidrug resistance has been reported worldwide. There is a need to implement alternative drug monitoring analytical tests. The objective of this study was to determine the consistency (5 repetitions) of the larval migration on agar test (LMAT) using ivermectin, moxidectin, pyrantel or albendazole against cyathostomin infective-stage larvae in eight different concentrations. LMAT showed a strong coefficient of determination (R2 > 0.91), between the test repetitions (n=5). The average 50% effective concentration (EC50) for ivermectin, moxidectin, pyrantel and albendazole were 0.0404, 0.0558, 0.0864 and 0.0988 nMol, respectively. The results of the EC50 for albendazole showed the greatest range of concentration. Ivermectin and moxidectin had the lowest in between-test variation. In the future, internationally certified susceptible isolates could be used for screening new drug candidates, or to follow up the pattern of drug efficacy from field populations. |
topic |
Cavalos anti-helmínticos teste in vitro parasitos |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000100091&lng=en&tlng=en |
work_keys_str_mv |
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