Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population.
OBJECTIVE: The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci. METHODS: Polymorphisms at -31C/T an...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4164436?pdf=render |
id |
doaj-56c0b551f1cd489b9a45dc02b4040117 |
---|---|
record_format |
Article |
spelling |
doaj-56c0b551f1cd489b9a45dc02b40401172020-11-24T21:38:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10691910.1371/journal.pone.0106919Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population.Xuefeng WangFengli JiangYu LiangLina XuHongbo LiYali LiuShiguo LiuYuanhua YeOBJECTIVE: The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci. METHODS: Polymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP) in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(X2) tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2. RESULTS: Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: X2 = 11.478, P = 0.003; For IL-1β-511T/C: X2 = 9.687, P = 0.008). LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D' = 0.92, r2 = 0.79). Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016). CONCLUSION: Our results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE.http://europepmc.org/articles/PMC4164436?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xuefeng Wang Fengli Jiang Yu Liang Lina Xu Hongbo Li Yali Liu Shiguo Liu Yuanhua Ye |
spellingShingle |
Xuefeng Wang Fengli Jiang Yu Liang Lina Xu Hongbo Li Yali Liu Shiguo Liu Yuanhua Ye Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. PLoS ONE |
author_facet |
Xuefeng Wang Fengli Jiang Yu Liang Lina Xu Hongbo Li Yali Liu Shiguo Liu Yuanhua Ye |
author_sort |
Xuefeng Wang |
title |
Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. |
title_short |
Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. |
title_full |
Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. |
title_fullStr |
Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. |
title_full_unstemmed |
Interleukin-1β-31C/T and -511T/C polymorphisms were associated with preeclampsia in Chinese Han population. |
title_sort |
interleukin-1β-31c/t and -511t/c polymorphisms were associated with preeclampsia in chinese han population. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
OBJECTIVE: The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci. METHODS: Polymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR- RFLP) in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(X2) tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2. RESULTS: Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: X2 = 11.478, P = 0.003; For IL-1β-511T/C: X2 = 9.687, P = 0.008). LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D' = 0.92, r2 = 0.79). Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016). CONCLUSION: Our results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE. |
url |
http://europepmc.org/articles/PMC4164436?pdf=render |
work_keys_str_mv |
AT xuefengwang interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT fenglijiang interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT yuliang interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT linaxu interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT hongboli interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT yaliliu interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT shiguoliu interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation AT yuanhuaye interleukin1b31ctand511tcpolymorphismswereassociatedwithpreeclampsiainchinesehanpopulation |
_version_ |
1725934752127516672 |